Kiwifruit SVP2 gene prevents premature budbreak during dormancy

Wu, Rui; Wang, Tianchi; Warren, Ben; Allan, Andrew C.; Macknight, Richard C.; Varkonyi‐Gasic, Erika

doi:10.1093/jxb/erx014

Cited by 73 publications

(68 citation statements)

References 74 publications

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“…The low regeneration efficiency was consistent with previous reports (Sasaki et al, 2011; Wu et al, 2017), suggesting that overexpression of SVP -like genes strongly inhibits outgrowth of shoots in tissue culture, which may explain the limited number of reported transgenic approaches to functionally characterize SVP/DAM genes in woody perennial species. To our knowledge, this is the first report of homologous overexpression of SVP -like genes in a horticultural tree.…”

Section: Discussionsupporting

confidence: 91%

“…Overexpression of Japanese apricot DAM6 in transgenic poplar resulted in premature growth cessation and terminal bud set in autumn and delayed shoot outgrowth in spring (Sasaki et al, 2011). However, in kiwifruit, SVP2 performed as a growth repressor once dormancy has been established, but appeared insufficient to suppress growth in permissive conditions (Wu et al, 2017). The current study suggests that MdDAMb and MdSVPa genes play similar growth inhibition roles in apple, without the capacity to promote growth cessation, consistent with their seasonal expression patterns.…”

Section: Discussionmentioning

confidence: 99%

“…The growth inhibitory function was also confirmed in kiwifruit. Overexpression of Actinidia chinensis SVP3 retarded reproductive development in transgenic A. eriantha (Wu et al, 2014) and overexpression of SVP2 delayed budbreak in transgenic A. deliciosa (Wu et al, 2017). …”

Section: Introductionmentioning

confidence: 99%

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SVP-like MADS Box Genes Control Dormancy and Budbreak in Apple

et al. 2017

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The annual growth cycle of trees is the result of seasonal cues. The onset of winter triggers an endodormant state preventing bud growth and, once a chilling requirement is satisfied, these buds enter an ecodormant state and resume growing. MADS-box genes with similarity to Arabidopsis SHORT VEGETATIVE PHASE (SVP) [the SVP-like and DORMANCY ASSOCIATED MADS-BOX (DAM) genes] have been implicated in regulating flowering and growth-dormancy cycles in perennials. Here, we identified and characterized three DAM-like (MdDAMs) and two SHORT VEGETATIVE PHASE-like (MdSVPs) genes from apple (Malus × domestica ‘Royal Gala’). The expression of MdDAMa and MdDAMc indicated they may play a role in triggering autumn growth cessation. In contrast, the expression of MdDAMb, MdSVPa and MdSVPb suggested a role in maintaining bud dormancy. Consistent with this, ectopic expression of MdDAMb and MdSVPa in ‘Royal Gala’ apple plants resulted in delayed budbreak and architecture change due to constrained lateral shoot outgrowth, but normal flower and fruit development. The association of MdSVPa and MdSVPb expression with floral bud development in the low fruiting ‘Off’ trees of a biennial bearing cultivar ‘Sciros’ suggested the SVP genes might also play a role in floral meristem identity.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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SVP-like MADS Box Genes Control Dormancy and Budbreak in Apple

et al. 2017

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“…Homologues of Arabidopsis SHORT VEGETATIVE PHASE ( SVP )/ AGAMOUS ‐ like 24 ( AGL24 ) encode the key regulators of dormancy and bud break in perennials (Bielenberg et al, ; Niu et al, ; Sasaki et al, ; Wu et al, ). In rosaceous species, these genes are also known as dormancy ‐ associated MADS ‐ box ( DAM ) genes.…”

Section: Introductionmentioning

confidence: 99%

“…The DAM / SVP ‐ like genes are considered to be the growth repressors of the peach evergrowing mutant lacking six tandem DAM genes. The ectopic expression of Prunus DAM6 in poplar and apple, and the overexpression of AcSVP2 in kiwifruit inhibit bud growth and enhance bud dormancy (Sasaki et al, ; Wu et al, ; Yamane et al, ). Recent studies involving poplar proved that the SVP ‐ like gene SVL mediates the photoperiod‐ and low‐temperature‐related regulation of apical bud dormancy (Singh et al, ; Singh, Miskolczi, Maurya, & Bhalerao, ).…”

Section: Introductionmentioning

confidence: 99%

ABA‐responsive ABRE‐BINDING FACTOR3 activatesDAM3expression to promote bud dormancy in Asian pear

Yang

et al. 2020

Plant Cell & Environment

101

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Bud dormancy is indispensable for the survival of perennial plants in cold winters.Abscisic acid (ABA) has essential functions influencing the endo-dormancy status. Dormancy-associated MADS-box/SHORT VEGETATIVE PHASE-like genes function downstream of the ABA signalling pathway to regulate bud dormancy. However, the regulation of DAM/SVP expression remains largely uncharacterized. In this study, we confirmed that endo-dormancy maintenance and PpyDAM3 expression are controlled by the ABA content in pear (Pyrus pyrifolia) buds. The expression of pear ABRE-BINDING FACTOR3 (PpyABF3) was positively correlated with PpyDAM3 expression. Furthermore, PpyABF3 directly bound to the second ABRE in the PpyDAM3 promoter to activate its expression. Interestingly, both PpyABF3 and PpyDAM3 repressed the cell division and growth of transgenic pear calli. Another ABA-induced ABF protein, PpyABF2, physically interacted with PpyABF3 and disrupted the activation of the PpyDAM3 promoter by PpyABF3, indicating DAM expression was precisely controlled. Additionally, our results suggested that the differences in the PpyDAM3 promoter in two pear cultivars might be responsible for the diversity in the chilling requirements. In summary, our data clarify the finely tuned regulatory mechanism underlying the effect of ABA on DAM gene expression and provide new insights into ABA-related bud dormancy regulation. K E Y W O R D S ABF3, Abscisic acid, bud dormancy, DAM, pear

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The MADS‐Box Gene Family Involved in the Regulatory Mechanism of Dormancy and Flowering in Rosaceae Fruit Trees

Hsiang

Yamane

2021

Annual Plant Reviews Online

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Temperate perennial fruit tree species enter dormancy in autumn. A prolonged exposure to chilling followed by warm conditions induce dormancy release and bud break. In Rosaceae species, flowering proceeds during dormancy when bud growth is repressed. TheSHORT VEGETATIVE PHASE(SVP)‐clade MADS‐box genes such asDORMANCY‐ASSOCIATED MADS‐boxes(DAM/SVPs) encode tree bud dormancy regulators. The major effects of DAM/SVPs include growth inhibition and bud break repression through the regulation of plant hormone metabolism in vegetative and floral buds. In addition, theFLOWERING LOCUS C(FLC)‐clade MADS‐box genes were recently also proposed to encode flowering and dormancy regulators. Apple (Malus domestica) FLC may have multifaceted roles in the juvenile–adult phase transition and in the annual development‐dormancy cycle in floral buds. Specifically, in apple, we hypothesize FLC3‐like may facilitate flowering in autumn, whereas an FLC‐like (FLC2) may inhibit bud break, thereby helping floral buds to remain in a quiescent state in winter and preventing an unexpected bud break before the spring. We developed a working model in which abscisic acid,C‐repeat Binding Factor(CBF),TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTOR(TCP), micro ribonucleic acid (microRNAs), and epigenetic regulation mediate processes that convert environmental signals into the transcriptional regulation ofDAMs andFLCs, thereby ensuring dormancy phase transitions and progression. Finally, possible relationships between MADS‐box genes and dormancy‐associated cellular metabolism are discussed.

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Kiwifruit SVP2 gene prevents premature budbreak during dormancy

Abstract: Overexpression of SVP2 in kiwifruit delays budbreak before sufficient winter chilling. SVP2-mediated vegetative growth restriction involves stress response pathways, and commonalities exist between Arabidopsis and kiwifruit SVP targets.

Cited by 73 publications

References 74 publications

SVP-like MADS Box Genes Control Dormancy and Budbreak in Apple

SVP-like MADS Box Genes Control Dormancy and Budbreak in Apple

ABA‐responsive ABRE‐BINDING FACTOR3 activatesDAM3expression to promote bud dormancy in Asian pear

The MADS‐Box Gene Family Involved in the Regulatory Mechanism of Dormancy and Flowering in Rosaceae Fruit Trees

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