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Fehér, Attila; Pasternak, Taras; Dudits, Dénes

doi:10.1023/a:1024033216561

Cited by 570 publications

(183 citation statements)

References 226 publications

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“…Auxins are considered a major factor evoking embryogenic competence in somatic tissues to induce somatic embryogenesis (Feher et al 2003;Gaj 2004). However, explants of different types respond differently to various auxins.…”

Section: Effect Of Auxins On Different Explants For Callus Inductionmentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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Dioscorea rotundata, commonly known as white yam, is an important staple food crop widely cultivated in West Africa and provides food security to millions of people. Genetic improvements of this crop using the advanced biotechnology tools have been hampered hitherto by the recalcitrant nature of regeneration through somatic embryogenesis. Therefore, we have developed an efficient and reproducible system for plant regeneration via somatic embryogenesis. Explants of different types (immature leaf, node, stem internode, root segment, petiole, and axillary bud) of D. rotundata accession TDr 2436 were tested for their embryogenic potentials on Murashige and Skoog (MS) medium supplemented with various auxins (2,4-D, NAA, and picloram). Among all explants tested, axillary bud explants cultured on MS medium supplemented with picloram (0.5-12 mg/l) favored the production of calli. Maximum proliferation of calli (526 mg fresh weight/explant) was achieved on MS medium supplemented with picloram (0.5 mg/l), casein hydrolysate (600 mg/l), and proline (1 g/l). Histology analysis confirmed that the embryogenic calli produced on this medium were mixed with non-embryogenic calli. Transfer of calli on MS basal medium supplemented with activated charcoal (1 %) changed the color of calli to purple and promoted the production of somatic embryos (87 embryos/callus) as well as adventitious shoot buds. Furthermore, upon transfer to MS medium supplemented with BAP (0.4 mg/l), the embryos continued their differentiation and maturation and germinated into complete plantlets. The adventitious shoot buds produced multiple shoots on MS medium supplemented with BAP (0.4 mg/l). Well-developed germinated plantlets were acclimatized in the screen house with 90 % survivability. Histology studies confirmed that the regeneration of D. rotundata reported here followed dual regeneration pathways. The embryogenic calli regenerated through development of somatic embryos and germinated into complete plantlets, however non-embryogenic calli regenerated through organogenesis and developed multiple shoots. The developed protocol has potential for somatic hybridization, mass clonal propagation, and genetic transformation applications.

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Section: Effect Of Auxins On Different Explants For Callus Inductionmentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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“…Various systems have been exploited to understand the mechanisms of gene regulation during SE and carrot has served as the model system [26,42,73,74,150,152,228]. Analysis of the proteome and transcriptome has led to the identification and characterization of certain genes involved in SE [7,21,22,26,57,77,107,154,158,167,187,207,220,226].…”

Section: Genes and Proteinsmentioning

confidence: 99%

“…Members of the SnRK group of kinases are thought to play a role in stress responses of plants, and some of them are induced by Abscisic Acid (ABA) [71]. The connection between stress and SE has received increasing attention over recent years [42].…”

Section: Mtsk1genementioning

confidence: 99%

“…Zygotic embryo development begins with the formation of the zygote following fertilization, while somatic cells acquire embryogenic competence as a result of different chemical and physical stimuli. Thus, plant SE is a developmental process involving the reprogramming of gene expression patterns involving cascades of genetic triggers turning on and off the expression of specific genes [42,127].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, these advances will benefit the lives of humans, animals, plants, agriculture, and the environment. Some reviews have been published dealing specifically with the molecular basis of vegetative-to-embryogenic transition [26,42,127,150]. Although recent analysis of the proteome and transcriptome have led to the identification and characterization of new genes induced SE, there is no review on recent information of prime events SE induction.…”

Section: Introductionmentioning

confidence: 99%

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Molecular aspects of somatic-to-embryogenic transition in plants

2009

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Somatic embryogenesis (SE) is a model system for understanding the physiological, biochemical, and molecular biological events occurring during plant embryo development. Plant somatic cells have the ability to undergo sustained divisions and give rise to an entire organism. This remarkable feature is called plant cell totipotency. SE is a notable illustration of plant totipotency and involves reprogramming of development in somatic cells toward the embryogenic pathway. Plant growth regularities, especially auxins, are key components as their exogenous application recapitulates the embryogenic potential of the mitotically quiescent somatic cells. It has been observed that there are genetic and also physiological factors that trigger in vitro embryogenesis in various types of plant somatic cells. Analysis of the proteome and transcriptome has led to the identification and characterization of certain genes involved in SE. Most of these genes, however, are upregulated only in the late developmental stages, suggesting that they do not play a direct role in the vegetative-to-embryogenic transition. However, the molecular bases of those triggering factors and the genetic and biochemical mechanisms leading to in vitro embryogenesis are still unknown. Here, we describe the plant factors that participate in the vegetative-to-embryogenic transition and discuss their possible roles in this process.

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