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Sushamakumari, S.; Asokan, M; Anthony, Paul; Lowe, K. C.; Power, J. B.; Davey, M. R.

doi:10.1023/a:1006494404224

Cited by 18 publications

(4 citation statements)

References 11 publications

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“…'PB 260' (Blanc et al, 2002;Modeste et al, 2012). The other sources of explants, i.e., protoplast (cell suspension) (Sushamakumari et al, 2000) and uninucleate anther (immature male flower bud) (Srichuay et al, 2014;Zhao et al, 2015;Wang et al, 2017) have been successfully inoculated for somatic embryogenesis. Somaclonal variations depend on i) presence of strong auxins [2,4-D and 3,4dichlorophenoxyacetic acid (3,4-D)] in inoculated medium for embryogenic callus and somatic embryogenesis induction (Modeste et al, 2013), ii) genotypes/clones (Srichuay et al, 2014), and iii) secondary somatic embryogenesis (subculture cycles) that ensures genetic fidelity in each plant derived from somatic embryogenesis (Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

True-to-type micropropagated plants of para rubber (Hevea brasiliensis Müll. Arg.) via somatic embryogenesis

Tisarum

Samphumphuang

Prommee

et al. 2020

Not Bot Horti Agrobo

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Plant micropropagation via somatic embryogenesis is a powerful technique for rapid mass propagation, especially in para rubber (Hevea brasiliensis Müll. Arg.). However, somaclonal variations are the major limitation of this process. To date, DNA fingerprinting, i.e., RAPD (Randomly Amplified Polymorphic DNA), Star Codon Targeted (SCoT), and SSRs (Simple Sequence Repeats), is one of the most successful technologies to detect the genetic fidelity in the somatic embryos. The aim of present study was to induce somatic embryos from inner integument explants of para rubber cv. ‘RRIM 600’ at different developmental stages and subsequent acclimatization and transplantation (under greenhouse and field conditions) of the propagated seedlings. The genetic stability of the plants derived from somatic embryos was also analysed in comparison to the mother plant using RAPD, SCoT and SSRs markers. Somatic embryos derived from inner integuments of 5-week-old immature seeds after pollination were more efficient than older and younger seeds. In addition, para rubber mother plants cv. ‘RRIM600’ and plants derived from somatic embryogenesis demonstrated the same pattern of DNA fragments, as confirmed by three PCR-based techniques, RAPD, SCoT and SSRs, whereas these in the pattern were different from ‘RRIT 226’, ‘PB 235’, ‘PB 251’, ‘PB 255’ and ‘BMP 24’. Interestingly, T2 plant was found to possess somaclonal variations when compared with mother plant. Based on the results, we confirm that the plants derived from somatic embryogenesis of para rubber cv. ‘RRIM 600’ were true-to-type to that of ‘RRIM 600’ master stock.

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Section: Discussionmentioning

confidence: 99%

True-to-type micropropagated plants of para rubber (Hevea brasiliensis Müll. Arg.) via somatic embryogenesis

Tisarum

Samphumphuang

Prommee

et al. 2020

Not Bot Horti Agrobo

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“…To date, the capacity to regenerate an entire plant through SE has been reported in various plants, including Arabidopsis, Gossypium, and Liquidambar hybrids, which has not only provided an effective method for virus-free plant propagation but has also greatly assisted gene transformation in many plant species [19,20]. Plant regeneration systems via SE in H. brasiliensis have been established using different kinds of explants, including anther-derived callus [12], inner integument-derived callus [13], secondary somatic embryos [14], leaf [21], embryogenic cell suspension lines [22], and protoplasts [23].…”

Section: Somatic Embryogenesis In H Brasiliensismentioning

confidence: 99%

Current Achievements and Future Challenges of Genotype-Dependent Somatic Embryogenesis Techniques in Hevea brasiliensis

Wang,

Gu,

et al. 2023

Forests

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The rubber tree (Hevea brasiliensis) is the most important commercial plant for producing natural rubber. Immature seed inner integument and anther-derived somatic embryogenesis techniques play a crucial role in the in vitro large-scale propagation and genetic transformation of the rubber tree. However, somatic embryogenesis is highly genotype-dependent, that is, only a limited number of H. brasiliensis genotypes, such as CATAS73397, CATAS917, and PB260, can be efficiently induced by somatic embryogenesis and used for large-scale propagation or transformation. The genotype dependence of the somatic embryogenesis technique is a conundrum for the application of Hevea biotechnology in most commercially important cultivars, such as Reken628 and CATAS879. Previous studies have shown that several somatic embryogenesis regulators can overcome genotype dependence and enhance the transformation and regeneration efficiency of recalcitrant plants and cultivars. In this review, we first describe the relevant successful applications of somatic embryogenesis technology in seedling production and genetic modification of H. brasiliensis. Second, we discuss the genotype dependence of somatic embryogenesis as the major challenge currently. Third, we summarize the recent significant advances in the understanding of the molecular mechanisms underlying somatic embryogenesis in other plants. Finally, we suggest a roadmap for using somatic embryogenesis regulatory genes to facilitate genotype-dependent somatic embryogenesis technology in H. brasiliensis.

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“…Sushamakumari et al (2000) showed for the first time how to regenerate plants from protoplast of H. brasiliensis . Embryogenic calli were induced on immature inflorescences and inner integuments of immature seeds.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Somatic Embryogenesis as Key Technology for Shaping the Rubber Tree of the Future

Mignon¹,

Werbrouck

2018

Front. Plant Sci.

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Worldwide, Hevea producers face the need to replant large surfaces in the coming years. The rubber yield per ha, produced by trees grafted on heterogeneous illegitimate seedling rootstocks, has reached its maximum. For long-standing Hevea clones, as for a lot of other tree species, one of the consequences of physiological aging is reduced in vitro growth and the lack of a proper geotropic (tap) root system. Somatic embryogenesis on young inner seed integument or stamen filaments provides a mean to regain ontogenetic juvenility. The process is limited by irregular germination of the somatic embryos. Nevertheless, with the obtained in vitro plants, juvenile lines have been established of the most important profitable rubber tree clones. Currently they are micropropagated on a commercial scale. Moreover, the produced plants can serve as mother plants for propagation by means of macro-cutting. Somatic embryogenesis enables the production of transgenic Hevea brasiliensis as well. Genes conferring plant disease resistance, abiotic stress tolerance and production of foreign proteins in the lactiferous vessels will further shape the rubber tree of the future.

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Cited by 18 publications

References 11 publications

True-to-type micropropagated plants of para rubber (Hevea brasiliensis Müll. Arg.) via somatic embryogenesis

True-to-type micropropagated plants of para rubber (Hevea brasiliensis Müll. Arg.) via somatic embryogenesis

Current Achievements and Future Challenges of Genotype-Dependent Somatic Embryogenesis Techniques in Hevea brasiliensis

Somatic Embryogenesis as Key Technology for Shaping the Rubber Tree of the Future

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