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Iranpour, Farhad Golshan; Nasr-Esfahani, Mohammad Hossein; Valojerdi, Mojtaba Rezazadeh; Al-Taraihi, Taki Mohammad Taki

doi:10.1023/a:1009406231811

Cited by 91 publications

(34 citation statements)

References 15 publications

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“…Sperm from infertile patients often exhibit anomalies linked to the type and proportion of protamines present (de Yebra et al, 1993;Carrell and Liu, 2001;Nasr-Esfahani et al, 2004;Aoki et al, 2006;Oliva, 2006;Torregrosa et al, 2006;Zhang et al, 2006;Singleton et al, 2007;Gázquez et al, 2008;Zini et al, 2008b). The present prospective cohort study could identify a correlation between abnormal chromatin packaging and sperm concentration (confirming the work of others; Lolis et al, 1996;Iranpour et al, 2000). Moreover, poor chromatin packaging was linked to a higher risk for DNA fragmentation (%DFI) and poorer DNA maturity (%HDS), confirming the work of others (Tavalaee et al, 2008).…”

Section: Discussionsupporting

confidence: 86%

“…Clear relationships have been demonstrated between CMA 3 staining and sperm morphology (Bianchi et al, 1996;Franken et al, 1999;Esterhuizen et al, 2000a). Poor chromatin packaging may contribute to failure of sperm decondensation in IVF or ICSI and may subsequently result in fertilization failure (Bianchi et al, 1996;Lolis et al, 1996;Sakkas et al, 1996;Esterhuizen et al, 2000b;Iranpour et al, 2000;Nasr-Esfahani et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Chromomycin A3 staining, sperm chromatin structure assay and hyaluronic acid binding assay as predictors for assisted reproductive outcome

Nijs

Creemers

Cox

et al. 2009

Reproductive BioMedicine Online

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Functional sperm tests such as the sperm chromatin structure assay (SCSA), chromomycin A3 staining (CMA(3)) and hyaluronic acid binding assay (HBA) have been suggested as predictive tests of fertility in vitro. This study aimed to define the clinical role of these functional parameters in assisted reproduction in a prospective cohort study. Conventional sperm diagnosis (motility, morphology and concentration) as well as SCSA, CMA(3) and HBA tests were performed on 205 semen samples [74 IVF, 94 ICSI and 37 combined IVF/intracytoplasmic sperm injection (ICSI)]. Main outcome parameters were fertilization rate, clinical pregnancy rate and take-home baby rate. The study showed that each of the three functional sperm tests was related to one or more conventional and one or more functional sperm tests, indicating that spermatozoa from patients with abnormal conventional semen parameters have a higher likelihood for multiple functional abnormalities. Only SCSA and CMA(3) staining were shown to have a limited predictive value when IVF or combined IVF/ICSI was applied. The proposed threshold value of

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Chromomycin A3 staining, sperm chromatin structure assay and hyaluronic acid binding assay as predictors for assisted reproductive outcome

Nijs

Creemers

Cox

et al. 2009

Reproductive BioMedicine Online

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show abstract

“…This link between defective spermiogenesis and DNA damage is further supported by the fact that several independent studies have also recorded correlations between DNA damage in human spermatozoa and elements of the conventional semen profile (specifically sperm count and morphology), which, in turn, reflect the efficiency of the spermatogenic process (Lolis et al 1996, Iranpour et al 2000, Irvine et al 2000. Spermiogenesis is itself highly susceptible to oxidative stress because isolated spermatids have a limited capacity for both DNA repair and glutathione replenishment (Den Boer et al 1990).…”

Section: Apoptosis In the Germ Linementioning

confidence: 92%

Apoptosis in the germ line

Aitken¹,

Findlay²,

Hutt³

et al. 2011

Reproduction

162

102

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Apoptosis is a critical process for regulating both the size and the quality of the male and female germ lines. In this review, we examine the importance of this process during embryonic development in establishing the pool of spermatogonial stem cells and primordial follicles that will ultimately define male and female fertility. We also consider the importance of apoptosis in controlling the number and quality of germ cells that eventually determine reproductive success. The biochemical details of the apoptotic process as it affects germ cells in the mature gonad still await resolution, as do the stimuli that persuade these cells to commit to a pathway that leads to cell death. Our ability to understand and ultimately control the reproductive potential of male and female mammals depends upon a deeper understanding of these fundamental processes.

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“…[22] Since fluorescence increase relative to CMA3 is an indicator of protamine deficiency in sperm nucleus chromatin, this study aimed to investigate the ultrastructure difference of sperm nucleus in samples with high and low CMA3 samples.…”

Section: Introductionmentioning

confidence: 99%

The effects of protamine deficiency on ultrastructure of human sperm nucleus

Iranpour

2014

Adv Biomed Res

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Background:Chromomycin A3 (CMA3) staining is one of the staining methods for detecting protamine deficiency in sperm nucleus. CMA3 is a fluorochrome that competes with protamines for binding to DNA double helix. It has been shown in our previous studies that percentage of CMA3 positive spermatozoa in semen has a close significant relationship with the fertilization rate in in vitro fertilization (IVF). The aim of this study was to examine the ultrastructural differences between sperms in patients who had high fluorescent percentages of yellow or red in CMA3 staining (protamine deficient) with patients with low fluorescent percentages.Materials and Methods:Semen samples are taken from five patients with high fluorescent percentages and five patients with low fluorescent percentages. Then the samples are passed for the different steps of preparing for electron microscopy. After the sectioning and mounting on grids, they are investigated under the transmission electron microscope.Results:Sperms in patients with low percentages of positive spermatozoa often have a normal appearance. Sperms in high fluorescent samples frequently have unpacked chromatin. Furthermore acrosomes of these sperms are thinner or disturbed. Also sometimes there are irregularities in sperm head membrane.Conclusion:Protamine deficiency in sperm nucleus can cause ultrastructural anomalies in sperm chromatin such as unpacking of it. It also is concomitant with acrosome and sperm membrane disturbances.

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Abstract: There is a close relationship among fertilization rate, sperm parameters, and CMA3 positivity and CMA3 could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.

Cited by 91 publications

References 15 publications

Chromomycin A3 staining, sperm chromatin structure assay and hyaluronic acid binding assay as predictors for assisted reproductive outcome

Chromomycin A3 staining, sperm chromatin structure assay and hyaluronic acid binding assay as predictors for assisted reproductive outcome

Apoptosis in the germ line

The effects of protamine deficiency on ultrastructure of human sperm nucleus

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