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Abstract

The antioxidative effect of intact cells and intracellular cell-free extracts of intestinal lactic acid bacteria B. longum (ATCC 15708) and L. acidophilus (ATCC 4356) was investigated. Both intact cells and intracellular cell-free extracts of 10(9)cells of B. longum and L. acidophilus demonstrated antioxidative activity, inhibiting linoleic acid peroxidation by 28-48%. This indicated that these two strains demonstrated excellent antioxidative activity. B. longum and L. acidophilus also showed the ability to scavenge alpha,alpha-diphenyl-beta-picrylhydrazyl (DPPH) free radical, scavenging 21-52%. The intact cells of these two intestinal bacteria demonstrated a high inhibitory effect on the cytotoxicity of 4-nitroquinoline-N-oxide (4NQO). Cytotoxicity of 4NQO was reduced by L. acidophilus by approximately half and by almost 90% by B. longum. Nevertheless, no inhibition of cytoxicity observed for intracellular cell-free extracts of 10(9) cells of B. longum and L. acidophilus. The effect of B. longum and L. acidophilus on inhibiting plasma lipid peroxidation was also evaluated. The results showed that both intestinal strains were able to protect plasma lipid from oxidation at different degrees. The inhibition rates on plasma lipid peroxidation ranged from 11 to 29% for 10(9) cells of B. longum and L. acidophilus. Generally speaking, B. longum demonstrated better antioxidative ability than L. acidophilus in this study.

Probiotic and Antioxidative Properties ofL. LactisLL27 Isolated from Milk

“…Free radical scavenging is one of the mechanisms by which antioxidants inhibit lipid peroxidation (32,45). An in vitro ability of Lactobacillus and Bifidobacterium to quench free radicals has been reported in a few studies as well (29,57). These data, together with our findings, suggest that the DPPh radical scavenging activity is genus-, strain-and dosedependent.…”

“…lactis LL27 was analyzed by a modifi cation method utilized by Blois (5) and lin and chang (29). the scavenged DPPh was then monitored by measuring the decrease in absorbance at 517 nm.…”

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

“…Lactic acid bacteria (LAB) are often used as supplements in probiotic products. The therapeutic effects of LAB include improvement of lactose intolerance, prevention of intestinal infection, decrease in serum cholesterol, increase in immune response, anticarcinogenic activity, and antioxidative effects (Lin and Chang, 2000).…”

Kefir, Kımız, Süt ve Yoğurt Uygulamasının Fare Dalağında Plazma Hücresi ve Mast Hücrelerinin Dağılımı Üzerine Etkileri

“…Beyond the inherent high nutritional value as a source of protein and calcium, kefir is believed to be beneficial to health in the countries where kefir is an essential part of the diet culture for a long time [26,27] . Effects of kefir on antioxidant and growth factors have been shown in several studies [22,[28][29][30][31][32][33] found that the protective effect of kefir is more than vitamin E in oxidative damage induced by CCl 4 in mice, by reducing lipid peroxidation and increasing reduced glutathione and glutathione peroxidase levels. In recent years in the mentality of a healthy diet, nutrition support with antioxidant-rich foods to prevent damages causing oxidative stress in the body has risen to the forefront.…”