Klebsiella oxytoca: an efficient pyrene-degrading bacterial strain isolated from petroleum-contaminated soil

Alfaify, Abdulkhaleg M.; Mir, Mushtaq Ahmad; Alrumman, Sulaiman A.

doi:10.1007/s00203-022-02850-9

Cited by 11 publications

(1 citation statement)

References 29 publications

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“…The phenacetin concentration in the enrichment culture was determined by UV/Vis spectrophotometry. The enrichment process was repeated five times to ensure the enrichment of cultures with phenacetin-degrading strains [ 26 , 27 ], after which the enriched culture was serially diluted and spread onto MSM agar plates containing 100 mg/L phenacetin and incubated at 30 °C for 3–7 days until colonies appeared. Different colonies were selected from each MSM plate and isolated by streak plating, with this process repeated three consecutive times to ensure that the selected colonies were pure and not contaminated [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of the Wastewater Micropollutant Phenacetin-Degrading Bacterium Rhodococcus sp. Strain PNT-23

Yuan,

Wang,

Liu

et al. 2023

Microorganisms

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Phenacetin, an antipyretic and analgesic drug, poses a serious health risk to both humans and aquatic organisms, which is of concern since this micropollutant is frequently detected in various aquatic environments. However, rare pure bacterial cultures have been reported to degrade phenacetin. Therefore, in this study, the novel phenacetin-degrading strain PNT-23 was isolated from municipal wastewater and identified as a Rhodococcus sp. based on its morphology and 16S rRNA gene sequencing. The isolated strain could completely degrade 100 mg/L phenacetin at an inoculum concentration of OD600 1.5 within 80 h, utilizing the micropollutant as its sole carbon source for growth. Strain PNT-23 exhibited optimal growth in LB medium at 37 °C and a pH of 7.0 with 1% NaCl, while the optimal degradation conditions in minimal medium were 30 °C and a pH of 7.0 with 1% NaCl. Two key intermediates were identified during phenacetin biodegradation by the strain PNT-23: N-acetyl-4-aminophenol and 4-aminophenol. This study provides novel insights into the biodegradation of phenacetin using a pure bacterium culture, expands the known substrate spectra of Rhodococcus strains and presents a potential new candidate for the microbial removal of phenacetin in a diverse range of environments.

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Section: Methodsmentioning

confidence: 99%