Klebsiella pneumoniae co-producing KPC and RmtG, finally targeting Switzerland

Mancini, Stefano; Poirel, Laurent; Corthesy, Myriam; Greub, Gilbert; Nordmann, Patrice

doi:10.1016/j.diagmicrobio.2017.10.019

Cited by 10 publications

(9 citation statements)

References 14 publications

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“…Such a frequent association between carbapenemase-producing and RMTase-producing Enterobacterales was already reported in different parts of the world [5][6][7][8][9][10][11][12][13]21,[27][28][29][30][31][32][33][34][35][36][37]. The origin of those isolates being identified in Switzerland could be autochthonous theoretically; nevertheless, clinical information (only partially obtained and therefore not presented in that study) showed that many of them corresponded to imported cases, and particularly from Eastern European countries, from Italy, and from the Indian subcontinent.…”

Section: Discussionmentioning

confidence: 72%

Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017–2020

et al. 2022

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Aminoglycosides (AGs) in combination with β-lactams play an important role in antimicrobial therapy in severe infections. Pan-resistance to clinically relevant AGs increasingly arises from the production of 16S rRNA methylases (RMTases) that are mostly encoded by plasmids in Gram-negative bacteria. The recent emergence and spread of isolates encoding RMTases is worrisome, considering that they often co-produce extended-spectrum β-lactamases (ESBLs) or carbapenemases. Our study aimed to retrospectively analyze and characterize the association of carbapenem- and aminoglycoside-resistant clinical isolates in Switzerland during a 3.5-year period between January 2017 and June 2020. A total of 103 pan-aminoglycoside- and carbapenem-resistant clinical isolates were recovered at the NARA (Swiss National Reference Center for Emerging Antibiotic Resistance) during the 2017–2020 period. Carbapenemase and RMTase determinants were identified by PCR and sequencing. The characterization of plasmids bearing resistance determinants was performed by a mating-out assay followed by PCR-based replicon typing (PBRT). Clonality of the isolates was investigated by multilocus sequence typing (MLST). Over the 991 Enterobacterales collected at the NARA during this period, 103 (10.4%) of them were resistant to both carbapenems and all aminoglycosides. Among these 103 isolates, 35 isolates produced NDM-like carbapenemases, followed by OXA-48-like (n = 23), KPC-like (n = 21), or no carbapenemase (n = 13), OXA-48-like and NDM-like co-production (n = 7), and VIM-like enzymes (n = 4). The RMTases ArmA, RmtB, RmtC, RmtF, RmtG, and RmtB + RmtF were identified among 51.4%, 13.6%, 4.9%, 24.3%, 1%, and 1%, respectively. Plasmid co-localization of the carbapenemase and the RMTase encoding genes was found among ca. 20% of the isolates. A high diversity was identified in terms of the nature of associations between RMTase and carbapenemase-encoding genes, of incompatibility groups of the corresponding plasmids, and of strain genetic backgrounds, highlighting heterogeneous importations rather than clonal dissemination.

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Section: Discussionmentioning

confidence: 72%

Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017–2020

et al. 2022

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“…The occurrence of Rmtg is apparently more restricted to South America, although it has been recently reported in europe (Mancini et al 2018). Because of the risk involved in the spread of resistance genes such as those associated with 16S-RMTase production, continuous monitoring is required to prevent global transmission.…”

Section: Introductionmentioning

confidence: 92%

“…Recently, Rmtg/D has been identified in P. aeruginosa and Klebsiella aerogenes (Francisco et al 2015(Francisco et al , grazziotin et al 2016. Regarding its genetic location, different Inc group plasmids have been shown to carry rmtG (N, A/C, B/O) (Bueno et al 2013, Mancini et al 2018, often within transposons containing additional resistance determinants.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of 16S rRNA methyltransferase in Brazil: RmtG in Klebsiella aerogenes ST93 (CC4)

Passarelli‐Araujo

Palmeiro

Moharana

et al. 2019

An. Acad. Bras. Ciênc.

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Aminoglycosides are a class of antibiotics that play a key role in antimicrobial treatment of Multidrug resistant (MDR) Gram-negative bacilli, typically in combination with β-lactams. Ribosomal 16S RNA modification by methyltransferases (e.g. RmtG) is an aminoglycoside resistance mechanism that, along with the occurrence carbapenem-resistant enterobacteriaceae (CRe), has become a clinical concern. In Brazil, rmtg genes were initially reported in Klebsiella pneumoniae, and monitoring isolates from other species carrying this gene is critical for epidemiological studies and to prevent dissemination. Here we report the presence of rmtg in Klebisella aerogenes D3 and characterize its genetic context in comparison to isolates from other species. Further, we performed a phylogenetic reconstruction of 900 16S rRNA methyltransferases (16S-RMTases) and methyltransferase-related proteins. We show that, in K. aerogenes D3, rmtg co-occurs with sul2, near a transposon with an IS91-like insertion sequence. Resistome analysis revealed the co-production of Rmtg and CTX-M-59. Ongoing surveillance of 16S-RMTases is crucial to delay the dissemination of such multiresistant isolates. Our results also highlight the reduction in treatment options for CRe infections, as well as the need of expanding prevention measures of these pathogens worldwide.

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“…Klebsiella pneumoniae is a Gram-negative nosocomial pathogen detected in many infectious diseases, such as bacteremia, pneumonia, urinary tract infections, and in patients with underlying diseases (Shakib et al, 2012;Mancini et al, 2018). The pathogenicity of this organism is notable because of its ability to produce some important enzymes which destroy an extended spectrum of antimicrobial agents (Shakib et al, 2012).…”

Section: Klebsiella Speciesmentioning

confidence: 99%

(GTG)5-PCR Mediated Molecular Typing of Zoonotic Bacteria

Babazadeh¹,

Ranjbar²

2021

WVJ

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The present review aimed to reveal the role of (GTG)5-PCR microbial typing in indicating the routes and source of infections, investigate the outbreaks and genotypes of clinical strains, as well as finding virulent strains and epidemiology of bacterial isolates. All available and published data in Google scholar, PubMed, ResearchGate, and Science Direct during the past two decades that used the (GTG)5-PCR method for genotyping the bacterial isolates were included in the current study. The findings have indicated that (GTG)5-PCR can be recommended as a possible, cost-effective, fast, and easy tool for molecular typing of bacterial isolates.

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Klebsiella pneumoniae co-producing KPC and RmtG, finally targeting Switzerland

Cited by 10 publications

References 14 publications

Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017–2020

Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017–2020

Molecular epidemiology of 16S rRNA methyltransferase in Brazil: RmtG in Klebsiella aerogenes ST93 (CC4)

(GTG)5-PCR Mediated Molecular Typing of Zoonotic Bacteria

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