Klebsiella pneumoniae Infection on a Rehabilitation Unit: Comparison of Epidemiologic Typing Methods

Thompson, William F.; Romance, L.; Bialkowska-Hobrazanska, H.; Rennie, R. P.; Ashton, F.; Nicolle, LE

doi:10.2307/30149729

Cited by 13 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amikacin and gentamicin are not commonly used antibiotics because of their potential nephrotoxicity, which might be a cause for this result. Previous studies have focused on the antibiotic resistance of P. aeruginosa (15)(16)(17)(18)(19) or K. pneumoniae in patients with SCI (16,(19)(20)(21)(22). Although Klebsiella was the second most common uropathogen seen in our SCI patients, its distribution was not significantly different between acute and chronic patients.…”

Section: Discussionmentioning

confidence: 64%

Retrospective Analysis of Nosocomial Urinary Tract Infections with Spinal Cord Injury Patients in a Rehabilitation Setting

Yılmaz¹,

Yavuz²,

Adıgüzel³

2014

Turk J Phys Med Rehab

View full text Add to dashboard Cite

Objective: To define the distribution and antibiotic susceptibility of nosocomial urinary tract infections (NAUTIs) in patients with spinal cord injury (SCI). Material and Methods: Records of the spinal cord injured patients were reviewed for NAUTIs between 2008 and 2010. Antibiotic resistance rates and factors that might affect urinary tract infection susceptibility were investigated retrospectively. Results: The data of 276 patients were included, of whom 159 were acute SCI patients and 117 were chronic SCI patients; 166 (61%) infections were asymptomatic bacteriuria (ASB), and 110 (39%) infections were symptomatic urinary tract infections (SUTIs). In patients with acute SCI, E. coli was the most frequently isolated pathogen of SUTIs, followed by Pseudomonas. In patients with chronic SCI, E. coli was the most frequently isolated pathogen in SUTIs and ASBs. There was no statistically significant difference in the rates of NAUTI (ASB and SUTI) between acute and chronic SCI patients in terms of bladder drainage method. The susceptibility ratios for E. coli were very low in both acute and chronic SCI patients using Foley catheters. Although the susceptibility ratios for E. coli were not that low in SCI patients using clean intermittent catheterization, the resistance ratios were higher than 50% for most of the uropathogens. Conclusion: These results reveal a blunt truth that the treatment of NAUTIs will be a great challenge in near future. Kronik SKY hastalarda SUTI ve ASB için en sık izole edilen patojen E. coli idi. Mesane boşaltım yöntemi dikkate alındığında, akut ve kronik SKY hastalar arasında NAUTI (ASB ve SUTI) dağılımı açısından anlamlı bir fark yoktu. Foley kateter kullanan akut ve kronik SKY hastalarda E. coli duyarlılık oranları çok düşüktü. Temiz aralıklı kateterizasyon uygulanan hastalarda duyarlılık oranları o kadar düşük olmasa da, direnç oranları üropatojenlerin çoğu için %50'nin üzerindeydi. Sonuç: Bu sonuçlar, nozokomiyal üriner sistem infeksiyonlarının tedavisinin yakın gelecekte ne kadar büyük bir sorun olacağı konusundaki gerçeği ortaya koymaktadır. Anahtar Kelimeler: Spinal kord yaralanması, nozokomiyal üriner sistem infeksiyonu, rehabilitasyon

show abstract

Section: Discussionmentioning

confidence: 64%

Retrospective Analysis of Nosocomial Urinary Tract Infections with Spinal Cord Injury Patients in a Rehabilitation Setting

Yılmaz¹,

Yavuz²,

Adıgüzel³

2014

Turk J Phys Med Rehab

View full text Add to dashboard Cite

show abstract

“…In epidemiological investigations, we therefore advocate two primers routinely for RAPD typing with perhaps a reserve primer for equivocal results. With two primers we achieved 100% typability, a rate unmatched by capsular serotyping, bacteriocin and bateriophage typing of K. pneumoniae [4][5][6]. There are two possible reasons why RAPD with HI-RP primer indicated M3 to be distinct from MI, M4 and M6 whereas all four isolates had identical CHEF profiles and RAPD profiles with Ut primer.…”

Section: Resultsmentioning

confidence: 99%

“…Biotyping and antibiogram typing are too limited in their ability to discriminate between strains to be used for the epidemiological typing of K. pneumoniae [2,4]. Whereas capsular serotyping, phage typing and bacteriocin typing have more discriminatory power, all three are time consuming and expensive methods and not all strains of K. pneumoniae are successfully typed by these methods [2,[4][5][6]. At present the most discriminatory method of typing K. pneumoniae involves the use of restriction fragment length polymorphism (RFLP) techniques [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…Whereas capsular serotyping, phage typing and bacteriocin typing have more discriminatory power, all three are time consuming and expensive methods and not all strains of K. pneumoniae are successfully typed by these methods [2,[4][5][6]. At present the most discriminatory method of typing K. pneumoniae involves the use of restriction fragment length polymorphism (RFLP) techniques [6][7][8]. However, these techniques are costly, labour-intensive and have lengthy processing times [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Randomly amplified polymorphic DNA typing: a useful tool for rapid epidemiological typing ofKlebsiella pneumoniae

et al. 1994

View full text Add to dashboard Cite

SUMMARYDiscriminatory typing methods are invaluable in the investigation of outbreaks of infectious diseases. Single primers were used to generate randomly amplified polymorphic DNA (RAPD) profiles from Klebsiella pneumoniae isolates of various serotype and K. pneumroniae isolates from cases of sepsis at a Malaysian hospital and two English hospitals. RAPD profiles of acceptable reproducibility, a maximum of three minor band variations, were produced using a rapid DNA extraction method. RAPD typing of K. pneumoniae was shown to be as discriminatory as restriction fragment length polymorphism analysis using pulsed field gel electrophoresis yet quicker and less costly. The findings suggest that RAPD typing may be a useful tool for the epidemiological typing of K. pneumoniae.

show abstract

“…(Table II). The first report was published in 1993 and did not appear to involve an ESBL-producing K. pneumoniae clone [16]. Nine reports described outbreak clones that produced various ESBLs or carbapenemases including SHV-4 [17], SHV-2 [8], an unidentified ESBL [2], the AmpC beta-lactamase ACC-1 [18], KPC-2 carbapenemase and SHV-5 [19], NDM carbapenemase [20], NDM-1 carbapenemase, CTX-M-15 and OXA-1 [21], KPC carbapenemases [22], and CTX-M-15 and OXA-1 [23].…”

Section: Outbreak Evolution and Infection Controlmentioning

confidence: 95%

Long-lasting outbreak due to CTX-M-15-producing Klebsiella pneumoniae ST336 in a rehabilitation ward: report and literature review

Valsdóttir

Jelle

Guðlaugsson

et al. 2017

Journal of Hospital Infection

View full text Add to dashboard Cite

Klebsiella pneumoniae Infection on a Rehabilitation Unit: Comparison of Epidemiologic Typing Methods

Cited by 13 publications

References 28 publications

Retrospective Analysis of Nosocomial Urinary Tract Infections with Spinal Cord Injury Patients in a Rehabilitation Setting

Retrospective Analysis of Nosocomial Urinary Tract Infections with Spinal Cord Injury Patients in a Rehabilitation Setting

Randomly amplified polymorphic DNA typing: a useful tool for rapid epidemiological typing ofKlebsiella pneumoniae

Long-lasting outbreak due to CTX-M-15-producing Klebsiella pneumoniae ST336 in a rehabilitation ward: report and literature review

Contact Info

Product

Resources

About