Klebsiella pneumoniae susceptibility to biocides and its association with cepA, qacΔE and qacE efflux pump genes and antibiotic resistance

Abuzaid, Abdulmonem A.; Hamouda, Ahmed; Amyes, Sebastian G. B.

doi:10.1016/j.jhin.2012.03.003

Cited by 89 publications

(71 citation statements)

References 21 publications

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“…Carrying the cepA and qac⌬E genes has been associated previously with such a phenotype (20). On the other hand, the insertional mutation leading to colistin resistance in our study was different from those recently described in colistin-and chlorhexidine-cross-resistant K. pneumoniae isolates, and the level of chlorhexidine nonsusceptibility did not reach the levels noted in that study (21).…”

Section: Pan-resistant Klebsiella Pneumoniae Clone In the Uaecontrasting

confidence: 97%

Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an IS Ecp1 -Directed bla _OXA-181 Insertion in the mgrB Gene in the United Arab Emirates

Sonnevend

Ghazawi

Hashmey

et al. 2017

Antimicrob Agents Chemother

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The emergence of pan-resistant Klebsiella pneumoniae strains is an increasing concern. In the present study, we describe a cluster of 9 pan-resistant K. pneumoniae sequence type 147 (ST147) isolates encountered in 4 patients over nearly 1 year in 3 hospitals of the United Arab Emirates (UAE). The isolates exhibited highly similar genotypes. All produced chromosomally encoded OXA-181, and the majority also produced the NDM-5 carbapenemase. As with the previously described single isolate from the UAE, MS6671, the mgrB was disrupted by a functional, ISEcp1-driven bla OXA-181 insertion causing resistance to carbapenems. The mutation was successfully complemented with an intact mgrB gene, indicating that it was responsible for colistin resistance. bla NDM-5 was located within a resistance island of an approximately 100-kb IncFII plasmid carrying ermB, mph(A), bla TEM-1B , rmtB, bla NDM-5 , sul1, aadA2, and dfrA12 resistance genes. Sequencing this plasmid (pABC143-NDM) revealed that its backbone was nearly identical to that of plasmid pMS6671E from which several resistance genes, including bla NDM-5 , had been deleted. More extensive similarities of the backbone and the resistance island were found between pABC143C-NDM and the bla NDM-5 -carrying IncFII plasmids of two K. pneumoniae ST147 isolates from South Korea, one of which was colistin resistant, and both also produced OXA-181. Notably, one of these strains was isolated from a patient transferred from the UAE. Our data show that this pan-resistant clone has an alarming capacity to maintain itself over an extended period of time and is even likely to be transmitted internationally.

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Section: Pan-resistant Klebsiella Pneumoniae Clone In the Uaecontrasting

confidence: 97%

Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an IS Ecp1 -Directed bla _OXA-181 Insertion in the mgrB Gene in the United Arab Emirates

Sonnevend

Ghazawi

Hashmey

et al. 2017

Antimicrob Agents Chemother

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“…The effect of biocides in antibiotic resistance has been explored in different studies specifically focused on particular situations of cross-resistance or induction of resistance (2,23,(36)(37)(38)(39). In this study, we went one step further and comprehensively analyzed the high diversity of effects exerted by several biocides and antibiotics on the most relevant enterobacterial pathogenic species, E. coli and K. pneumoniae.…”

Section: Discussionmentioning

confidence: 99%

“…However, only a few studies with limited data have been performed with either E. coli or K. pneumoniae, and less is known in the case of clinical isolates (19,(22)(23)(24)(25)(26)(27). The aim of this study was to determine the effect of preexposure to the more commonly used biocides in hospitals and domestic settings (TRI, CHX, benzalkonium chloride [BKC], and sodium hypochlorite [SHC]), or to antibiotics of wide consumption (ampicillin [AMP] and ciprofloxacin [CIP]) on the selection of biocide-resistant variants and to explore the associated changes in metabolism and bacterial growth rate.…”

mentioning

confidence: 99%

Polymorphic Variation in Susceptibility and Metabolism of Triclosan-Resistant Mutants of Escherichia coli and Klebsiella pneumoniae Clinical Strains Obtained after Exposure to Biocides and Antibiotics

Curião

Marchi

Viti

et al. 2015

Antimicrob Agents Chemother

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Exposure to biocides may result in cross-resistance to other antimicrobials. Changes in biocide and antibiotic susceptibilities, metabolism, and fitness costs were studied here in biocide-selected Escherichia coli and Klebsiella pneumoniae mutants. E. coli and K. pneumoniae mutants with various degrees of triclosan susceptibility were obtained after exposure to triclosan (TRI), benzalkonium chloride (BKC), chlorhexidine (CHX) or sodium hypochlorite (SHC), and ampicillin or ciprofloxacin. Alterations in antimicrobial susceptibility and metabolism in mutants were tested using Phenotype MicroArrays. The expression of AcrAB pump and global regulators (SoxR, MarA, and RamA) was measured by quantitative reverse transcription-PCR (qRT-PCR), and the central part of the fabI gene was sequenced. The fitness costs of resistance were assessed by a comparison of relative growth rates. Triclosan-resistant (TRI r ) and triclosan-hypersusceptible (TRI hs ) mutants of E. coli and K. pneumoniae were obtained after selection with biocides and/or antibiotics. E. coli TRI r mutants, including those with mutations in the fabI gene or in the expression of acrB, acrF, and marA, exhibited changes in susceptibility to TRI, CHX, and antibiotics. TRI r mutants for which the TRI MIC was high presented improved metabolism of carboxylic acids, amino acids, and carbohydrates. In TRI r mutants, resistance to one antimicrobial provoked hypersusceptibility to another one(s). TRI r mutants had fitness costs, particularly marAoverexpressing (E. coli) or ramA-overexpressing (K. pneumoniae) mutants. TRI, BKC, and CIP exposure frequently yielded TRI r mutants exhibiting alterations in AraC-like global regulators (MarA, SoxR, and RamA), AcrAB-TolC, and/or FabI, and influencing antimicrobial susceptibility, fitness, and metabolism. These various phenotypes suggest a trade-off of different selective processes shaping the evolution toward antibiotic/biocide resistance and influencing other adaptive traits.

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“…This resistance can affect public health because pathogenic infection is not treated by certain antibiotics [1]. Thus, studies on antibiotic resistance bacteria (ARB) have been growing in number [2][3][4] and have been highlighted in water and wastewater environments [5,6].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Communities and Antibiotic Resistance Communities in a Full-Scale Hospital Wastewater Treatment Plant by High-Throughput Pyrosequencing

Ahn

Choi

2016

Water

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Abstract:The community of whole microbes and antibiotic resistance bacteria (ARB) in hospital wastewater treatment plants (WWTP) receiving domestic wastewater (DWW) and hospital wastewater (HWW) was investigated. Samples from an influent of a secondary clarifier, at each treatment train, were characterized for the whole microbial community and ARB on the antibiotic resistance database, based on high-throughput pyrosequencing. The pyrosequencing analysis revealed that the abundance of Bacteroidetes in the DWW sample was higher (~1.6 times) than in the HWW sample, whereas the abundance of Proteobacteria in the HWW sample was greater than in the DWW sample. At the top twenty of the genus level, distinct genera were observed-Saprospiraceae in the DWW and Zoogloea in the HWW. Apart from the top twenty genera, minor genera showed various antibiotic resistance types based on the antibiotic resistance gene database.

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Klebsiella pneumoniae susceptibility to biocides and its association with cepA, qacΔE and qacE efflux pump genes and antibiotic resistance

Cited by 89 publications

References 21 publications

Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an IS Ecp1 -Directed bla _OXA-181 Insertion in the mgrB Gene in the United Arab Emirates

Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an IS Ecp1 -Directed bla _OXA-181 Insertion in the mgrB Gene in the United Arab Emirates

Polymorphic Variation in Susceptibility and Metabolism of Triclosan-Resistant Mutants of Escherichia coli and Klebsiella pneumoniae Clinical Strains Obtained after Exposure to Biocides and Antibiotics

Bacterial Communities and Antibiotic Resistance Communities in a Full-Scale Hospital Wastewater Treatment Plant by High-Throughput Pyrosequencing

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Klebsiella pneumoniae susceptibility to biocides and its association with cepA, qacΔE and qacE efflux pump genes and antibiotic resistance

Cited by 89 publications

References 21 publications

Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an IS Ecp1 -Directed bla OXA-181 Insertion in the mgrB Gene in the United Arab Emirates

Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an IS Ecp1 -Directed bla OXA-181 Insertion in the mgrB Gene in the United Arab Emirates

Polymorphic Variation in Susceptibility and Metabolism of Triclosan-Resistant Mutants of Escherichia coli and Klebsiella pneumoniae Clinical Strains Obtained after Exposure to Biocides and Antibiotics

Bacterial Communities and Antibiotic Resistance Communities in a Full-Scale Hospital Wastewater Treatment Plant by High-Throughput Pyrosequencing

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Product

Resources

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Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an IS Ecp1 -Directed bla _OXA-181 Insertion in the mgrB Gene in the United Arab Emirates

Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an IS Ecp1 -Directed bla _OXA-181 Insertion in the mgrB Gene in the United Arab Emirates