Klotho, BDNF, NGF, GDNF Levels and Related Factors in Withdrawal Period in Chronic Cannabinoid Users

Abstract

Klotho and neurotropic factors have recently been shown to be related to some psychiatric disorders and neurocognitive disorders, but there is no study on this issue within substance users. In this study, brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), glial derived neurotrophic factor (GDNF) and klotho serum levels of a patient group consisting of 27 chronic cannabis users according to the DSM-V and 27 healthy volunteers were compared, and their relationships with other the clinical features of other patients were evaluated. Clinical scales, the Buss-Perry Aggression Scale, and the Substance Craving Scale were repeated on the first day of hospitalisation and on the seventh day of withdrawal. BDNF, GDNF, NGF and klotho levels were analysed using the ELISA method. There was no differences between the cannabinoid use disorder group and the control group regarding their klotho and other neurotrophic levels, but initiation age of cannabis use was negatively correlated with these levels. In addition, there was a relationship between verbal aggression scores and BDNF and NGF levels. There was a positive correlation between klotho and neurotrophic factors in all groups (patient group Day 1, patient group Day 7, control group) (p \ 0.01). When comparing the difference between the correlations using the cocor (a comprehensive solution for the statistical comparison of correlations), the klotho-GDNF and klotho-NGF correlations for the first day of the patient group and the control group were different. In this study, rather than a difference in klotho levels and neurotropic factors, a significant relationship between these markers and each other and clinical parameters was demonstrated; further studies are needed to understand the exact mechanism.

Brain-Derived Neurotrophic Factor Levels in Cannabis Use Disorders - A Systematic Review and Meta-Analysis

“…Several studies have tested this hypothesis by measuring serum BDNF levels in cannabis users with variable results [10,11,[16][17][18][19][20][21]. Therefore, the aim of this systematic review is to investigate and summarize the existing literature regarding the levels of BDNF in individuals with cannabis use disorder.…”

Effect of cannabis use on blood levels of brain‐derived neurotrophic factor (BDNF) and nerve growth factor (NGF): A systematic review and meta‐analysis

“…Following the full‐text review, 11 studies met the inclusion criteria and were included in the quantitative synthesis. A flow diagram illustrating the study selection process is presented in Figure 1 (Angelucci et al., 2008 ; Bayazit et al., 2019 ; Chagas et al., 2014 ; D'Souza et al., 2009 ; Jockers‐Scherubl et al., 2004 , 2006 ; Lisano et al., 2020 ; Miguez et al., 2019 ; Szabo et al., 2020 ; Toll et al., 2020 ; Yazici et al., 2022 ).…”

Serum Klotho Level and its Related Factors Among Male Opioids Addicts With Normal Renal Function Compared to Healthy Male Non-smokers and Smokers in Tabriz, Iran

“…In the present study, younger addicts (aged 21-30 years) had higher Klotho level than older addicts (aged 31-40 years), but there was no significant difference in Klotho level between the age groups. Yazici et al in Turkey indicated a significant negative correlation between age and Klotho level among those with chronic cannabis use in the withdrawal period [23], which suggests the detrimental effect of cannabis on Klotho level in the body. According to our results, the serum level of Klotho was almost the same in male addicts and healthy men.…”