Kluyveromyces aestuarii, a potential environmental quality indicator yeast for mangroves in the State of Rio de Janeiro, Brazil

Araujo, F.V; Hagler, Allen N.

doi:10.1590/s1517-83822011000300014

Cited by 10 publications

(4 citation statements)

References 10 publications

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“…S1). In addition to K. lactis (10), Kluyveromyces aestuarii and M. fructicola had been described as producing pulcherrimin (11,12). We sequenced the genomes of two more budding yeast species: one previously described as producing an unknown red pigment, Zygotorulaspora mrakii (26), from which we identified a PUL gene cluster; one from the other recognized species of this genus, Zygotorulaspora florentina, a nonpigmented strain that did not contain a complete PUL cluster.…”

Section: Resultsmentioning

confidence: 99%

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Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts

Krause

Kominek

Opulente

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Secondary metabolites are key in how organisms from all domains of life interact with their environment and each other. The iron-binding molecule pulcherrimin was described a century ago, but the genes responsible for its production in budding yeasts have remained uncharacterized. Here, we used phylogenomic footprinting on 90 genomes across the budding yeast subphylum Saccharomycotina to identify the gene cluster associated with pulcherrimin production. Using targeted gene replacements in Kluyveromyces lactis, we characterized the four genes that make up the cluster, which likely encode two pulcherriminic acid biosynthesis enzymes, a pulcherrimin transporter, and a transcription factor involved in both biosynthesis and transport. The requirement of a functional putative transporter to utilize extracellular pulcherrimin-complexed iron demonstrates that pulcherriminic acid is a siderophore, a chelator that binds iron outside the cell for subsequent uptake. Surprisingly, we identified homologs of the putative transporter and transcription factor genes in multiple yeast genera that lacked the biosynthesis genes and could not make pulcherrimin, including the model yeast Saccharomyces cerevisiae. We deleted these previously uncharacterized genes and showed they are also required for pulcherrimin utilization in S. cerevisiae, raising the possibility that other genes of unknown function are linked to secondary metabolism. Phylogenetic analyses of this gene cluster suggest that pulcherrimin biosynthesis and utilization were ancestral to budding yeasts, but the biosynthesis genes and, subsequently, the utilization genes, were lost in many lineages, mirroring other microbial public goods systems that lead to the rise of cheater organisms.

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Section: Resultsmentioning

confidence: 99%

“…and some Kluyveromyces spp. (9)(10)(11)(12). Pulcherrimin is a red iron-containing pigment composed of two cyclized and modified leucine molecules (13).…”

mentioning

confidence: 99%

Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts

Krause

Kominek

Opulente

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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“…However, these studies fail to consider their role in marine habitats. An aspect that has to be considered is that many of these fungi may be passively washed into the marine milieu and survive as dormant propagules (Hagler et al 1982; Araujo and Hagler 2011;Fell 2014). While culture-independent studies of fungal diversity in the sea have discovered new lineages of fungi: a marine Chyridiomycota lineage in deep-sea sediments (Nagano et al 2010), an Bunknown phyllotypes branching within the basidiomycete radiation^in deepsea hydrothermal vent Le Calvez et al (2009) and 36 novel marine lineages (Richard et al 2012), but they fail to acknowledge the role of these fungi.…”

Section: Introductionmentioning

confidence: 99%

Classification of marine Ascomycota, Basidiomycota, Blastocladiomycota and Chytridiomycota

et al. 2015

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This paper lists the accepted names and classification of marine fungi, updating the scheme presented in 2009. The classification includes 1,112 species (in 472 genera): Ascomycota 805 (in 352 genera), Basidiomycota 21 species (in 17 genera), Chytridiomycota and related phyla 26 species (in 13 genera), Zygomycota three (in two genera), Blastocladiomycota one species (one genus), asexual morphs of filamentous fungi 43 (in 26 genera); and marine yeasts: Ascomycota 138 species (in 35 genera), Basidiomycota 75 species (in 26 genera). These fungi belong to 129 families and 65 orders. The Halosphaeriaceae remains the largest family of marine fungi with 141 species in 59 genera, while the most specious genera are Aspergillus (47 species), Penicillium (39 species) and the yeast genus Candida (64 species). The review includes details of recent higher order nomenclature changes, and accounts of new families, genera and species described over the past 5 years.

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“…appear to be unique in their synthesis of a separate siderophore, ferrichrome (Van Der Walt et al 1990 ), presumably for iron transport and iron storage as in Schizosaccharomyces pombe (Schrettl et al 2004 ). The latter results, and the wealth of Saccharomycotina species harboring genes for siderophore uptake (Kluyver et al 1953 , Araujo and Hagler 2011 , Lachance 2016 ), suggest a landscape of interactions by Saccharomycotina in communities based on iron nutrients (Thanh et al 2002 ), analogous to well-studied dynamics in bacteria (Kramer et al 2020 ).…”

Section: Biochemistry and Metabolismmentioning

confidence: 86%

Natural trait variation across Saccharomycotina species

Wang,

Steenwyk,

Brem

2024

FEMS Yeast Research

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Among molecular biologists, the group of fungi called Saccharomycotina is famous for its yeasts. These yeasts in turn are famous for what they have in common—genetic, biochemical, and cell-biological characters that serve as models for plants and animals. But behind the apparent homogeneity of Saccharomycotina species lie a wealth of differences. In this review, we discuss traits that vary across the Saccharomycotina subphylum. We describe cases of bright pigmentation; a zoo of cell shapes; metabolic specialties; and species with unique rules of gene regulation. We discuss the genetics of this diversity and why it matters, including insights into basic evolutionary principles with relevance across Eukarya.

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Kluyveromyces aestuarii, a potential environmental quality indicator yeast for mangroves in the State of Rio de Janeiro, Brazil

Cited by 10 publications

References 10 publications

Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts

Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts

Classification of marine Ascomycota, Basidiomycota, Blastocladiomycota and Chytridiomycota

Natural trait variation across Saccharomycotina species

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