Kluyveromyces marxianus as a Platform in Synthetic Biology for the Production of Useful Materials

Lertwattanasakul, Noppon; Nurcholis, Mochamad; Rodrussamee, Nadchanok; Kosaka, Tomoyuki; Murata, Masayuki; Yamada, Mamoru

doi:10.1007/978-3-030-89680-5_11

Cited by 5 publications

(4 citation statements)

References 225 publications

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“…The thermal stability of K. marxianus DMKU 3-1042 is one of the most important application criteria for different applications, and this property is crucial for the preparation strategy of the strain or its stabilization. The stability of K. marxianus DMKU 3-1042 is beneficial for a wide range of applications in synthesis processes, which currently are in great demand from the industrial point of view [10,21].…”

Section: Discussionmentioning

confidence: 99%

“…Kluyveromyces marxianus is a thermotolerant yeast that has beneficial properties for industrial ethanol fermentation, including efficient production of ethanol at high temperatures, high growth rate, short doubling times, weak glucose repression, and the ability to assimilate various sugars, such as glucose, xylose, and sucrose, that are present in various raw materials [1][2][3][4][5]. Studies on K. marxianus have been increasing rapidly, and the high Microorganisms 2022, 10, 798 2 of 19 potential of K. marxianus for industrial applications has been reviewed [6][7][8][9][10]. Among the K. marxianus strains studied, DMKU 3-1042, one of the most thermotolerant strains that was isolated in Thailand, has been extensively analyzed [1,7,11], and its complete genome sequence has been determined by transcriptomic analysis under four different growth conditions [12].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to temperature tolerance, fermenting microbes are required to be resistant to various stressors present during fermentation, such as ethanol, acetic acid, osmotic stress, and oxidative stress [13,19,20]. Therefore, HTF that uses stresstolerant microbes can reduce the total cost of ethanol production, bring cheaper ethanol compared to commercial materials, and provide benefits for industrial application [10,21].…”

Section: Introductionmentioning

confidence: 99%

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Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

et al. 2022

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During ethanol fermentation, yeast cells are exposed to various stresses that have negative effects on cell growth, cell survival, and fermentation ability. This study, therefore, aims to develop Kluyveromyces marxianus-adapted strains that are multi-stress tolerant and to increase ethanol production at high temperatures through a novel evolutionary adaptation procedure. K. marxianus DMKU 3-1042 was subjected to repetitive long-term cultivation with gradual increases in temperature (RLCGT), which exposed cells to various stresses, including high temperatures. In each cultivation step, 1% of the previous culture was inoculated into a medium containing 1% yeast extract, 2% peptone, and 2% glucose, and cultivation was performed under a shaking condition. Four adapted strains showed increased tolerance to ethanol, furfural, hydroxymethylfurfural, and vanillin, and they also showed higher production of ethanol in a medium containing 16% glucose at high temperatures. One showed stronger ethanol tolerance. Others had similar phenotypes, including acetic acid tolerance, though genome analysis revealed that they had different mutations. Based on genome and transcriptome analyses, we discuss possible mechanisms of stress tolerance in adapted strains. All adapted strains gained a useful capacity for ethanol fermentation at high temperatures and improved tolerance to multi-stress. This suggests that RLCGT is a simple and efficient procedure for the development of robust strains.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

et al. 2022

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“…There is a need for the use of cellulosic biomass, but the high cost of pretreatment and saccharifying enzymes is a problem. High-temperature fermentation (HTF) to produce bioethanol is attracting attention because it can reduce the costs of cooling, saccharifying enzymes used in simultaneous saccharification and fermentation, and equipment [2]. Thermotolerant Fuels 2023, 4 fermenting microorganisms are essential for HTF, but stable fermentation at high temperatures requires the use of microorganisms that are resistant not only to temperature but also to toxic substances present in the fermentation broth and have high ethanol productivity.…”

Section: Introductionmentioning

confidence: 99%

Mutants with Enhanced Multi-Stress Tolerance of Kluyveromyces marxianus and Their Ability for Ethanol Fermentation

Lertwattanasakul,

Pattanakittivorakul,

Nitiyon

et al. 2023

Fuels

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Kluyveromyces marxianus is an attractive thermotolerant yeast species for ethanol production because of its ability to utilize various carbon sources as a fermentation substrate. The use of thermotolerant microorganisms enables the performance of high-temperature ethanol fermentation, which has several advantages, including the reduction of cooling costs and minimization of contamination risks. To improve K. marxianus for ethanol fermentation under stress conditions, two strains, DMKU 3-1042 and DMKU 3-118, were adapted for heat resistance and resistance to toxic substances in pulp wastewater from a paper mill, respectively, resulting in the generation of KMR1042 and KMR118, respectively. Both adapted mutants exhibited clumpy clusters of cells as pseudo-hyphae and altered colony morphology, and their sedimentation speeds were much faster than those of the corresponding parent strains. The two mutants showed stronger tolerance to various stresses and higher performance for ethanol production than those of the corresponding parent strains at high temperatures or in the presence of toxic substances. Genome sequencing analysis revealed that both mutants had disruption of the same gene, SWI5, despite adaptation under different stress conditions, suggesting that the formation of pseudo-hyphae is a common strategy of K. marxianus for coping with stresses.

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Potential of Thermo-Tolerant Microorganisms for Production of Cellulosic Bioethanol

Lertwattanasakul

Rodrussamee

Kumakiri

et al. 2023

Handbook of Biorefinery Research and Technology

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Kluyveromyces marxianus as a Platform in Synthetic Biology for the Production of Useful Materials

Cited by 5 publications

References 225 publications

Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

Mutants with Enhanced Multi-Stress Tolerance of Kluyveromyces marxianus and Their Ability for Ethanol Fermentation

Potential of Thermo-Tolerant Microorganisms for Production of Cellulosic Bioethanol

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