Knee Loading Enhances the Migration of Adipose-Derived Stem Cells to the Osteoarthritic Sites Through the SDF-1/CXCR4 Regulatory Axis

Mechanical loading and orthobiologic therapies in the treatment of post-traumatic osteoarthritis (PTOA): a comprehensive review

“…Optimizing PTOA pathways of treatment includes understanding the role of mechanical loading following injection of orthobiologics. In terms of injectable orthobiologics, a study conducted in 2022 demonstrated the effectiveness of mechanical loading combined with intra-articular injection of adipose-derived MSC in an OA mice model induced by ACL tear ( Zhang et al, 2022 ). The study utilized loading at 1.0 N for 6 min at 5 Hz over a period of 2 weeks.…”

A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis

“…BMSCs (2 ​× ​10 4 ​cells/well) were suspended in 100 ​μL of serum-free culture medium with or without AMD3100 (an inhibitor of CXCR4) (10 ​μg/ml). Cells were placed into the upper chamber, and 500 μL DMEM medium containing 10% FBS was added to the bottom chamber [ 5 ]. After incubation at 37 ​°C and 5% CO 2 for 12 ​h, the upper surface of the transwell filters was swabbed to remove cells.…”

“…Thus, increasing the expression of CXCR4 is a potential strategy to improve the migration of BMSCs and accelerate tissue repair [ 22 ]. Our previous study has shown that physical stimulation can promote the migration of MSCs in OA mice by activating SDF-1/CXCR4 signaling [ 5 ]. In addition, the Piezo1 channel, as a key mechanical sensor, can convert mechanical stimuli into electrochemical signals in vivo [ 23 ].…”

“…As OA worsens, it is usually resolved with the surgical implantation of artificial joints [ 1 ]. However, artificial joints have a limited lifespan and are not suitable for the treatment of early OA [ 5 ]. Thus, an effective regimen to attenuate the pathological progress of OA is urgently needed.…”

Finding the Goldilocks Zone of Mechanical Loading: A Comprehensive Review of Mechanical Loading in the Prevention and Treatment of Knee Osteoarthritis

“…A similar study analyzed joint healing and adipose-derived stem cell (ASCs) migration after treatment with ASC injection and mechanical compression (1.0 N for 6 min at 5 Hz for 2 weeks) [146]. They found that joint loading provided increased ASC migration at the joint and accelerated repair of the OA-damaged sites.…”