2022
DOI: 10.3389/fpls.2022.878037
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Knock-Out of CmNAC-NOR Affects Melon Climacteric Fruit Ripening

Abstract: Fruit ripening is an important process that affects fruit quality. A QTL in melon, ETHQV6.3, involved in climacteric ripening regulation, has been found to be encoded by CmNAC-NOR, a homologue of the tomato NOR gene. To further investigate CmNAC-NOR function, we obtained two CRISPR/Cas9-mediated mutants (nor-3 and nor-1) in the climacteric Védrantais background. nor-3, containing a 3-bp deletion altering the NAC domain A, resulted in ~8 days delay in ripening without affecting fruit quality. In contrast, the 1… Show more

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Cited by 26 publications
(23 citation statements)
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“…While Ved ripened at around 35 days after pollination in both years, PS ripened around 20 days later, generally without producing aroma or abscission layer (Figure 1G-I) (Table S1). The ripening behavior observed for both parental lines was in agreement with previous studies [27,30,33].…”
Section: Phenotyping Of the Parental Linessupporting
confidence: 92%
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“…While Ved ripened at around 35 days after pollination in both years, PS ripened around 20 days later, generally without producing aroma or abscission layer (Figure 1G-I) (Table S1). The ripening behavior observed for both parental lines was in agreement with previous studies [27,30,33].…”
Section: Phenotyping Of the Parental Linessupporting
confidence: 92%
“…One was with the same parental lines, where the allele of PS delayed ripening in a Ved background [27], and another in an IL population with the same recurrent parent PS, where the introgression of the alleles of PI 161375 induced a climacteric response [53,54]. The causal gene for this QTL has been characterized and identified as transcription factor CmNAC-NOR [33,55]. The other chromosome governing climacteric ripening in this IL population was chromosome 8.…”
Section: Ripening Traitsmentioning
confidence: 98%
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“…Because the NAC family exists from aquatic green algae to higher terrestrial plants, its members participate in the formation of the organ boundaries of plants transiting from vegetative growth to reproductive growth and are related to flower development and fruit ripening, which are essential to crop yield and quality (Maugarny-Calès et al, 2016;Ma et al, 2017;Mathew and Agarwal, 2018;Forlani et al, 2021;Singh et al, 2021;Liu G. S. et al, 2022). Thereby, in recent years, much progress has been made toward understanding the molecular mechanisms regulated by NAC TFs in flower development (Guo et al, 2022;Liang et al, 2022;Liu X. et al, 2022;Wang et al, 2022;Ghissing et al, 2023) and fruit ripening (Fu B. L. et al, 2021;Gao et al, 2021;Kou et al, 2021;Gao et al, 2022;Liu B. et al, 2022;Qi et al, 2022). In this review, we mainly summarize up-to-date advances on the basic properties, function, and action mechanisms of NACs in flower formation, flowering, and fruit ripening to improve our understanding of the molecular basis of crop yield and quality mediated by NAC TFs.…”
Section: Introductionmentioning
confidence: 99%
“…The 30-bp deletion mutant destroys the metal-binding motif CysXXCys of OsHPP04. Usually the mutation of a few amino acids doesn’t affect protein function, however, many studies indicated that deletion of amino acids in key domains can disable protein function ( Yang et al., 2021 ; de Figueiredo et al., 2022 ; Liu et al., 2022a ). Therefore, the mutant line of 30-bp deletion (10 amino acids deletion) increased resistance against M. graminicola probably because of the 30-bp deletion which destroys the key domain of CysXXCys.…”
Section: Discussionmentioning
confidence: 99%