Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1

Gao, Weimin; Liu, Yongqing; Giometti, Carol S.; Tollaksen, Sandra L.; Khare, Tripti; Wu, Liyou; Klingeman, Dawn M.; Fields, Matthew W.; Zhou, Jizhong

doi:10.1186/1471-2164-7-76

Cited by 48 publications

(45 citation statements)

References 44 publications

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“…A mutagenesis system for constructing deletion mutants in S. oneidensis MR-1 has previously been developed and successfully utilized [23], [27]. The deletion was confirmed by PCR and DNA sequencing (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Functional Assessment of EnvZ/OmpR Two-Component System in Shewanella oneidensis

et al. 2011

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EnvZ and OmpR constitute the bacterial two-component signal transduction system known to mediate osmotic stress response in a number of Gram-negative bacteria. In an effort to understand the mechanism through which Shewanella oneidensis senses and responds to environmental osmolarity changes, structure of the ompR-envZ operon was determined with Northern blotting assay and roles of the EnvZ/OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR (qRT-PCR), and phenotype microarrays. Results from the mutational analysis and qRT-PCR suggested that the EnvZ/OmpR system contributed to osmotic stress response of S. oneidensis and very likely engaged a similar strategy employed by E. coli, which involved reciprocal regulation of two major porin coding genes. Additionally, the ompR-envZ system was also found related to cell motility. We further showed that the ompR-envZ dependent regulation of porin genes and motility resided almost completely on ompR and only partially on envZ, indicating additional mechanisms for OmpR phosphorylation. In contrast to E. coli lacking ompR-envZ, however, growth of S. oneidensis did not show a significant dependence on ompR-envZ even under osmotic stress. Further analysis with phenotype microarrays revealed that the S. oneidensis strains lacking a complete ompR-envZ system displayed hypersensitivities to a number of agents, especially in alkaline environment. Taken together, our results suggest that the function of the ompR-envZ system in S. oneidensis, although still connected with osmoregulation, has diverged considerably from that of E. coli. Additional mechanism must exist to support growth of S. oneidensis under osmotic stress.

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Section: Resultsmentioning

confidence: 99%

Functional Assessment of EnvZ/OmpR Two-Component System in Shewanella oneidensis

et al. 2011

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“…The correct mutagenesis vector, verified by DNA sequencing, was then transferred into S. oneidensis by conjugation for the subsequent steps of the fusion-PCR-based mutagenesis procedure [24]. …”

Section: Resultsmentioning

confidence: 99%

“…coli chromosome, has been developed and successfully utilized in this study. To construct Shewanella mutants, fusion PCR technique with various suicide plasmids has been the most frequently utilized [10,24,26,27]. Because of the large size of suicide plasmids and unwanted PCR byproducts, these methods with traditional cut-ligation cloning suffers from the lack of unique restriction sites and/or a low efficiency for ligation of the fusion PCR products.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…oneidensis in-frame deletion mutants involves with multiple rounds of PCR and conventional cloning, both of which greatly reduce its efficiency and effectiveness [24]. To make an improvement, in this study we developed a new mutagenesis-construct delivery vector employing attB - attP (BP) recombination based on the system reported before [24]. This plasmid, namely pHGM01, was created by introduction of a DNA fragment containing attP1 , ccdB , Cm R , and attP2 from pMK2010 into the Sac I site of pDS3.0 [23,24] (Figure 2A).…”

Section: Methodsmentioning

confidence: 99%

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Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis

et al. 2013

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Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be intact, thereby likely functional. Previously, in-frame deletion mutants for 36 of these genes were obtained and characterized. In this study, first we completed the construction of an entire set of c-type cytochrome mutants utilizing a newly developed att-based mutagenesis approach, which is more effective and efficient than the approach used previously by circumventing the conventional cloning. Second, we investigated the cytochrome c maturation (Ccm) system in S. oneidensis. There are two loci predicted to encode components of the Ccm system, SO0259-SO0269 and SO0476-SO0478. The former is proven essential for cytochrome c maturation whereas the latter is dispensable. Unlike the single operon organization observed in other γ-proteobacteria, genes at the SO0259-SO0269 locus are uniquely organized into four operons, ccmABCDE, scyA, SO0265, and ccmFGH-SO0269. Functional analysis revealed that the SO0265 gene rather than the scyA and SO0269 genes are relevant to cytochrome c maturation.

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Synthetic curli enables efficient microbial electrocatalysis with stainless‐steel electrode

Suo

Fang

et al. 2019

AIChE Journal

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Microbial electrocatalysis systems (MES) provide a cutting‐edge solution to global problems associated with the environment and energy, but practical applications are hindered by the expensive electrode materials. Although stainless steel (SS) has been proposed as a promising inexpensive candidate, poor cell/SS interaction results in a low performance for MES. Here, a new synthetic biology approach was established for reinforcing the cell/SS interaction. Hybridized curli nanofibers fused with a metal‐binding domain were heterogeneously expressed onto the cell surface, which realized efficient cell binding with the SS electrode. Consequently, it enabled a ~420‐fold improvement of the anodic power output and a substantial enhancement of the cathodic Coulombic efficiency (from 0.6 to 4% to over 80%) with an SS electrode. This work demonstrates low‐cost MES with an SS electrode and introduces a new avenue to engineer the cell/electrode interaction, which is promising for future practical applications of MES.

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Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1

Cited by 48 publications

References 44 publications

Functional Assessment of EnvZ/OmpR Two-Component System in Shewanella oneidensis

Functional Assessment of EnvZ/OmpR Two-Component System in Shewanella oneidensis

Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis

Synthetic curli enables efficient microbial electrocatalysis with stainless‐steel electrode

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