Knockdown of 14-3-3γ Suppresses Epithelial–Mesenchymal Transition and Reduces Metastatic Potential of Human Non-small Cell Lung Cancer Cells

Raungrut, Pritsana; Wongkotsila, Anusara; Champoochana, Nidanut; Lirdprapamongkol, Kriengsak; Svasti, Jisnuson; Thongsuksai, Paramee

doi:10.21873/anticanres.12622

Cited by 20 publications

(23 citation statements)

References 22 publications

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“…Baine MJ et al reported that the level of coagulation factor V was found to be significantly different in an analysis of peripheral monocytes from pancreatic cancer patients and might be related to tumor stage [22]. YWHAG is a member of a highly conserved family of proteins that participates in many intracellular signal transduction processes and plays an important role in cell survival and proliferation [23][24][25][26]. However, the role of YWHAG in pancreatic Fig.…”

Section: Discussionmentioning

confidence: 99%

Identification of dissociation factors in pancreatic Cancer using a mass spectrometry-based proteomic approach

et al. 2020

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Backgroud: Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome of pancreatic cancer is key to its progression and metastasis. But different methods of protein extraction affect the final results. In other words, the real secretion of proteins in cancer cells has been changed. Based on mass spectrometry, we analyze the secretome from the serum-containing and serum-free medium, using different protein pretreatment methods. This study aims to identify dissociation factors in pancreatic cancer. Methods: In this study, pancreatic cancer cells were cultured in serum-containing or serum-free medium, and the corresponding supernatants were extracted as samples. Subsequently, the above samples were separated by size exclusion chromatography (SEC), and peptide segments were identified by LC-MS/MS. The final results were identified via the hamster secreted protein database and a public database. Results: Although the number of identified proteins in the serum-free medium group was high, the real secretion of proteins in pancreatic cancer cells was changed. There were six significant secreted proteins in the serumcontaining medium group. Survival analysis via the TCGA database suggested that patients with higher expression levels of YWHAG showed a worse overall survival rate than those with lower YWHAG expression. Conclusions: Our study demonstrated the results in the serum-containing medium group were more similar to the real secretome of pancreatic cancer cells. YWHAG could be used as a prognostic indicator for pancreatic cancer.

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Section: Discussionmentioning

confidence: 99%

Identification of dissociation factors in pancreatic Cancer using a mass spectrometry-based proteomic approach

et al. 2020

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show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Dissociation Factors in Pancreatic Cancer using a Mass Spectrometry-Based Proteomic Approach

Liu

Kong

Liang

et al. 2020

Preprint

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Backgroud Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome of pancreatic cancer is key to its progression and metastasis. But different methods of protein extraction affect the final results. In other words, the real secretion of proteins in cancer cells has been changed. Based on mass spectrometry, we analyze the secretome from the serum-containing and serum-free medium, using different protein pretreatment methods. This study aims to identify dissociation factors in pancreatic cancer. Methods In this study, pancreatic cancer cells were cultured in serum-containing or serum-free medium, and the corresponding supernatants were extracted as samples. Subsequently, the above samples were separated by size exclusion chromatography (SEC), and peptide segments were identified by LC-MS/MS. The final results were identified via the hamster secreted protein database and a public database. Results Although the number of identified proteins in the serum-free medium group was high, the real secretion of proteins in pancreatic cancer cells was changed. There were six significant secreted proteins in the serum-containing medium group. Survival analysis via the TCGA database suggested that patients with higher expression levels of YWHAG showed a worse overall survival rate than those with lower YWHAG expression. Conclusions Our study demonstrated the results in the serum-containing medium group were more similar to the real secretome of pancreatic cancer cells. YWHAG could be used as a prognostic indicator for pancreatic cancer.

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“…14-3-3 subtypes are considered to play nocogenic roles in a variety of tumors (79). Raungrut et al (80) found that 14-3-3 gamma is involved in the metastasis of lung cancer cells, and found that knockdown of 14-3-3 gamma could inhibit lung cancer metastasis. The 14-3-3 beta protein has been shown to possess carcinogenic potential, and its increased expression has been detected in many types of cancers.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Ubiquitinated Proteins in Human Pituitary and Pituitary Adenoma Tissues

Qian

Zhan

et al. 2019

Front. Endocrinol.

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Protein ubiquitination is an important post-translational modification that is associated with multiple diseases, including pituitary adenomas (PAs). Protein ubiquitination profiling in human pituitary and PAs remains unknown. Here, we performed the first ubiquitination analysis with an anti-ubiquitin antibody (specific to K-ε-GG)-based label-free quantitative proteomics method and bioinformatics to investigate protein ubiquitination profiling between PA and control tissues. A total of 158 ubiquitinated sites and 142 ubiquitinated peptides in 108 proteins were identified, and five ubiquitination motifs were found. KEGG pathway network analysis of 108 ubiquitinated proteins identified four statistically significant signaling pathways, including PI3K-AKT signaling pathway, hippo signaling pathway, ribosome, and nucleotide excision repair. R software Gene Ontology (GO) analysis of 108 ubiquitinated proteins revealed that protein ubiquitination was involved in multiple biological processes, cellular components, and molecule functions. The randomly selected ubiquitinated 14-3-3 zeta/delta protein was further analyzed with Western blot, and it was found that upregulated 14-3-3 zeta/delta protein in nonfunctional PAs might be derived from the significantly decreased level of its ubiquitination compared to control pituitaries, which indicated a contribution of 14-3-3 zeta/delta protein to pituitary tumorigenesis. These findings provided the first ubiquitinated proteomic profiling and ubiquitination-involved signaling pathway networks in human PAs. This study offers new scientific evidence and basic data to elucidate the biological functions of ubiquitination in PAs, insights into its novel molecular mechanisms of pituitary tumorigenesis, and discovery of novel biomarkers and therapeutic targets for effective treatment of PAs.

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Knockdown of 14-3-3γ Suppresses Epithelial–Mesenchymal Transition and Reduces Metastatic Potential of Human Non-small Cell Lung Cancer Cells

Abstract: Our results suggest that knockdown of 14-3-3γ may be a potential strategy for suppressing metastasis of lung cancer by inhibiting MMP2 and MMP9 through regulation of EMT.

Cited by 20 publications

References 22 publications

Identification of dissociation factors in pancreatic Cancer using a mass spectrometry-based proteomic approach

Identification of dissociation factors in pancreatic Cancer using a mass spectrometry-based proteomic approach

Identification of Dissociation Factors in Pancreatic Cancer using a Mass Spectrometry-Based Proteomic Approach

Quantitative Analysis of Ubiquitinated Proteins in Human Pituitary and Pituitary Adenoma Tissues

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