Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli

Li, Shuangtao; Zhang, Lei; Wang, Ying; Xu, Feifei; Mengyun, Liu; Lin, Peng; Ren, Shuxin; Ma, Rui; Guo, Yang-Dong

doi:10.1038/srep41397

Cited by 20 publications

(13 citation statements)

References 58 publications

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“…Cellulose synthase ( CESA ) genes play roles in the synthesis of primary and secondary cell wall (Zhang et al , 2016). Secondary walls have a large impact on plant adaptation to the environment, and some CESA genes and cellulose synthase-like genes ( CSL ) are involved in salt tolerance (Zhu et al , 2010 a ; Zhu et al , 2010 b ; Wang et al ., 2016; Zhang et al , 2016; Li et al , 2017; Zhang et al , 2018). Our RNA-Seq data indicated that there were 31 cellulose synthase family genes down-regulated by AtHSFA7b (see Supplementary Table S5), indicating that they might be involved in AtHSFA7b-mediated salt tolerance.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression

Zang

Wang

Zhang

et al. 2019

Journal of Experimental Botany

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Plant heat shock transcription factors (HSFs) are involved in heat and other abiotic stress responses. However, their functions in salt tolerance are little known. In this study, we characterized the function of a HSF from Arabidopsis, AtHSFA7b, in salt tolerance. AtHSFA7b is a nuclear protein with transactivation activity. ChIP-seq combined with an RNA-seq assay indicated that AtHSFA7b preferentially binds to a novel cis-acting element, termed the E-box-like motif, to regulate gene expression; it also binds to the heat shock element motif. Under salt conditions, AtHSFA7b regulates its target genes to mediate serial physiological changes, including maintaining cellular ion homeostasis, reducing water loss rate, decreasing reactive oxygen species accumulation, and adjusting osmotic potential, which ultimately leads to improved salt tolerance. Additionally, most cellulose synthase-like (CSL) and cellulose synthase (CESA) family genes were inhibited by AtHSFA7b; some of them were randomly selected for salt tolerance characterization, and they were mainly found to negatively modulate salt tolerance. By contrast, some transcription factors (TFs) were induced by AtHSFA7b; among them, we randomly identified six TFs that positively regulate salt tolerance. Thus, AtHSFA7b serves as a transactivator that positively mediates salinity tolerance mainly through binding to the E-box-like motif to regulate gene expression.

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Section: Discussionmentioning

confidence: 99%

Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression

Zang

Wang

Zhang

et al. 2019

Journal of Experimental Botany

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“…Among the identified proteins in broccoli, cell wall cellulose protein was found. It has been suggested that the regulation of cell wall cellulose synthesis has an important role in polysaccharide metabolism and adaptations of broccoli plants to salt stress [ 52 ]. Knockdown mutants for the cellulose synthase gene showed overexpressed BoPIP2;2 and BoPIP2;3 aquaporins compared to the WT plants, pointing out that these two isoforms enhanced the salt tolerance of mutant plants.…”

Section: Discussionmentioning

confidence: 99%

Plasma membrane aquaporins mediates vesicle stability in broccoli

et al. 2018

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The use of in vitro membrane vesicles is attractive because of possible applications in therapies. Here we aimed to compare the stability and functionality of plasma membrane vesicles extracted from control and salt-treated broccoli. The impact of the amount of aquaporins was related to plasma membrane osmotic water permeability and the stability of protein secondary structure. Here, we describe for first time an increase in plant aquaporins acetylation under high salinity. Higher osmotic water permeability in NaCl vesicles has been related to higher acetylation, upregulation of aquaporins, and a more stable environment to thermal denaturation. Based on our findings, we propose that aquaporins play an important role in vesicle stability.

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“…8 by functional nominations. Similar functional genes have been reported controlling salt tolerance or abiotic stress in plants, such as Callose synthase which plays an important role in response to multiple biotic and abiotic stresses, including salt stress [57, 58]. In addition, V-type H + -transporting ATPase subunit expressed in the roots, energizes sodium sequestration into the central vacuole and enhances salt tolerance in plants [59–61].…”

Section: Discussionmentioning

confidence: 74%

Identification of new loci for salt tolerance in soybean by high-resolution genome-wide association mapping

et al. 2019

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Background Salinity is an abiotic stress that negatively affects soybean [ Glycine max (L.) Merr.] seed yield. Although a major gene for salt tolerance was identified and consistently mapped to chromosome (Chr.) 3 by linkage mapping studies, it does not fully explain genetic variability for tolerance in soybean germplasm. In this study, a genome-wide association study (GWAS) was performed to map genomic regions for salt tolerance in a diverse panel of 305 soybean accessions using a single nucleotide polymorphism (SNP) dataset derived from the SoySNP50K iSelect BeadChip. A second GWAS was also conducted in a subset of 234 accessions using another 3.7 M SNP dataset derived from a whole-genome resequencing (WGRS) study. In addition, three gene-based markers (GBM) of the known gene, Glyma03g32900 , on Chr. 3 were also integrated into the two datasets. Salt tolerance among soybean lines was evaluated by leaf scorch score (LSS), chlorophyll content ratio (CCR), leaf sodium content (LSC), and leaf chloride content (LCC). Results For both association studies, a major locus for salt tolerance on Chr. 3 was confirmed by a number of significant SNPs, of which three gene-based SNP markers, Salt-20, Salt14056 and Salt11655, had the highest association with all four traits studied. Also, additional genomic regions on Chrs. 1, 8, and 18 were found to be associated with various traits measured in the second GWAS using the WGRS-derived SNP dataset. Conclusions A region identified on Chr. 8 was identified to be associated with all four traits and predicted as a new minor locus for salt tolerance in soybean. The candidate genes harbored in this minor locus may help reveal the molecular mechanism involved in salt tolerance and to improve tolerance in soybean cultivars. The significant SNPs will be useful for marker-assisted selection for salt tolerance in soybean breeding programs. Electronic supplementary material The online version of this article (10.1186/s12864-019-5662-9) contains supplementary material, which is available to authorized users.

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Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli

Cited by 20 publications

References 58 publications

Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression

Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression

Plasma membrane aquaporins mediates vesicle stability in broccoli

Identification of new loci for salt tolerance in soybean by high-resolution genome-wide association mapping

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