Knockdown of Brachyury Suppresses Breast Cancer Cell Proliferation and Migration via Targeting E2F3

Chen, Ming; Liu, Jinyan; Liang, Xiao; Huang, Ying; Yang, Zhen; Lu, Pei Hsuan; Shen, Jun; Shi, Keqin; Qu, Huiheng

doi:10.1155/2022/7913067

Cited by 5 publications

(6 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7 In addition, Chen et al showed that the Brachyury/E2F3 axis might be a diagnostic biomarker, and Knockdown of E2F3 diminished BC cell growth and migration. 48 Moreover, according to Jusino et al, the expression of SGO1 and E2F3 increased in invasive ductal BC compared with normal breast tissues, which correlated significantly with poor survival outcomes, epithelial-tomesenchymal transition, and metastasis. 32 Furthermore, we reported the protein-protein interaction between E2F3 and P53, and the positive correlation between them.…”

Section: Discussionmentioning

confidence: 98%

Effect of microRNA-141-3p, E2F3, CDK3, and KAT2B overexpression on histologic tumor grade and metastasis status in untreated breast cancer tissues

Ebrahimian Vargahan,

Barati,

Roudbari

et al. 2024

Bioimpacts

View full text Add to dashboard Cite

Introduction: Increasing evidence has reported gene expression alterations in breast cancer (BC) tissues, necessitating their investigating to highlight the molecular basis of the disease development or progression. This study investigated the expression of miR-141, E2F3, CDK3, TP53, and KAT2B, and their association with histologic grade and metastasis in BC tissues. Methods: The RNA expression level of miR-141, E2F3, CDK3, TP53, and KAT2B genes was analyzed in 23 BC and 23 normal tissue samples by RT-qPCR. The associations of the expression level of these genes with clinicopathological features of the BC tissue samples were evaluated. The study also explored the correlation between RNA levels of genes and miR-141. Results: Expression of miR-141, E2F3, CDK3, and KAT2B demonstrated significantly higher levels in BC tumor than normal tissues. TP53 expression showed an increase in tumor compared to normal tissues, although it was insignificant. Moreover, increased RNA expression of miR-141, E2F3, CDK3, and KAT2B corresponded to the advanced stage and regional metastasis of BC. Additionally, the results demonstrated a significant correlation between RNA expression levels of miR-141 with CDK3 and E2F3 with KAT2B. Conclusion: Our findings highlighted clinicopathologic indicators that were relevant to aggressive BC. Besides, Correlations between overexpression of miR-141, E2F3, CDK3, and KAT2B in BC tissues suggest regulatory effects. Taken together, it seems results of this study could provide evidence that dysregulation of gene expression contributes significantly to unveiling the underlying molecular basis of BC.

show abstract

Section: Discussionmentioning

confidence: 98%

Effect of microRNA-141-3p, E2F3, CDK3, and KAT2B overexpression on histologic tumor grade and metastasis status in untreated breast cancer tissues

Ebrahimian Vargahan,

Barati,

Roudbari

et al. 2024

Bioimpacts

View full text Add to dashboard Cite

show abstract

“…For the subcutaneous tumor model [ 19 ], 1 × 10 7 cells transfected with sh-NRSN2-AS1 or sh-NC were subcutaneously injected into the left and right flanks of nude mice, respectively ( n = 5). The tumor volumes were calculated every 3 days (V = 0.5 × D × d 2 (V, volume; D, longitudinal diameter; d, latitudinal diameter)).…”

Section: Methodsmentioning

confidence: 99%

Long non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway

Wu,

Li,

Liu

et al. 2023

Cell Death Dis

View full text Add to dashboard Cite

As a common malignant tumor among women, ovarian cancer poses a serious threat to their health. This study demonstrates that long non-coding RNA NRSN2-AS1 is over-expressed in ovarian cancer tissues using patient sample and tissue microarrays. In addition, NRSN2-AS1 is shown to promote ovarian cancer cell proliferation and metastasis both in vitro and in vivo. Mechanistically, NRSN2-AS1 stabilizes protein tyrosine kinase 2 (PTK2) to activate the β-catenin pathway via repressing MG-53-mediated ubiquitinated degradation of PTK2, thereby facilitating ovarian cancer progression. Rescue experiments verify the function of the NRSN2-AS1/PTK2/β-catenin axis and the effects of MG53 on this axis in ovarian cancer cells. In conclusion, this study demonstrates the key role of the NRSN2-AS1/PTK2/β-catenin axis for the first time and explores its potential clinical applications in ovarian cancer.

show abstract

“…For the subcutaneous tumor model 15 , 1×10 7 cells transfected with sh-NRSN2-AS1 or sh-NC were subcutaneously injected into the left and right anks of nude mice, respectively (n = 5). The tumor volumes were calculated every 3 days (V = 0.5 × D × d 2 (V, volume; D, longitudinal diameter; d, latitudinal diameter)).…”

Section: Xenograft In Micementioning

confidence: 99%

Long Non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway

Shen,

Wu,

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Knockdown of Brachyury Suppresses Breast Cancer Cell Proliferation and Migration via Targeting E2F3

Cited by 5 publications

References 38 publications

Effect of microRNA-141-3p, E2F3, CDK3, and KAT2B overexpression on histologic tumor grade and metastasis status in untreated breast cancer tissues

Effect of microRNA-141-3p, E2F3, CDK3, and KAT2B overexpression on histologic tumor grade and metastasis status in untreated breast cancer tissues

Long non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway

Long Non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway

Contact Info

Product

Resources

About