Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level

Wei, Li; Shen, Chen; Hajjami, Mohamed El; You, Wuxin; Wang, Qintao; Zhang, Peng; Ji, Yicai; Hu, Hanhua; Hu, Qiang; Poetsch, Ansgar; Xu, Jian

doi:10.1016/j.ymben.2019.03.004

Cited by 65 publications

(42 citation statements)

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“…The mitochondrion may possess a potential biochemical pathway for xing inorganic carbon (HCO 3 -) postulated previously by us [23,29]. The carbon was xed by phosphoenolpyruvate carboxylase (PEPC, IMET_4839) converting phosphoenolpyruvate (PEP) to oxaloacetate (OAA) ( Figure 4B Figure 4B).…”

Section: Transcriptomics Overestimates Downregulation Of Light Harvesmentioning

confidence: 86%

“…- [34]. Five putative CAs (CA1: IMET_2109 CA2: IMET_5775; CA3: IMET_4525; CA4: IMET_1930, CA5: IMET_1034) are present in N. oceanica IMET1 [6,23] and predicted to reside in the chloroplast according to results from ChloroP, TargetP, Signal P and former research [41][42][43][44] (Supplementary Table 3). Under N-, on proteome level, CA1…”

Section: Transcriptomics Overestimates Downregulation Of Light Harvesmentioning

confidence: 99%

“…This is a striking difference physiological from C. reinhardtii,, which mostly accumulates starch in this situation [16,22] . Tools for strain engineering and single-cell based rapid phenotyping have been developed for this strain (e.g., CRISPR/Cas9 and ramanome analysis [23][24][25][26]).…”

Section: Introductionmentioning

confidence: 99%

“…xation [23,29,30] and TAG synthesis [31,32]. Therefore, here employing N. oceanica IMET1 as a model, we tracked the proteome pro les from 3 h, 6 h, 12 h, 24 h, 48 h up to 10 days during microalgal adaption to N-.…”

Section: Introductionmentioning

confidence: 99%

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Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

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Wei

Gong

et al. 2020

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Background: Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipid in the form of triacylglycerides (TAG). Several researches have studied the mechanism of this process from the perspective of transcriptome and metabolome, yet proteome analysis on this process is still sparse and lacking the analysis of the dynamic adaption to nitrogen deficiency. Here, proteomes for 03h, 06h, 12h, 24h, 48h and 10th day of nitrogen deplete and replete conditions were compared. New proteome results were integrated with existing transcriptome and other data. Results: Obtained results illuminated physiological adaptations not deduced from previous transcriptome data: (a) Abundance of proteins related to photosynthesis only slightly decreased in the first 48h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48h under nitrogen deficiency, suggesting that respiration is enhanced after 48h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48h, synchronous with the previously reported diversification of fatty acids after 48h. Conclusions: This study adds a proteome perspective on the major pathways for TAG accumulation by Nannochloropsis due to absence of nitrogen: photosynthesis, membrane lipid conversion, protein degradation, TCA cycle. By integrating existing transcriptome and other data, our research provided for Nannochloropsis oceanica a multi-layered description of adaptation to nitrogen limitation and lipid accumulation. Cluster analysis of this integrated dataset allowed inference of post-transcriptional regulation events.

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Section: Transcriptomics Overestimates Downregulation Of Light Harvesmentioning

confidence: 86%

Section: Transcriptomics Overestimates Downregulation Of Light Harvesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

You

Wei

Gong

et al. 2020

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“…oceanica IMET1 is an industrial oleaginous strain as TAG can represent up to 69% (dry matter) lipids [22]. Tools for strain engineering and single-cell based rapid phenotyping have been developed for this strain (e.g., CRISPR/Cas9 and ramanome analysis [23][24][25][26]). Moreover, cultivation methods have been optimized in large-scale bioreactors [3,27] and lipid extraction [28].…”

mentioning

confidence: 99%

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

You

Wei

Gong

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Background Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipid in the form of triacylglycerides (TAG). Several researches have studied the mechanism of this process from the perspective of transcriptome and metabolome, yet proteome analysis on this process is still sparse and lacking the analysis of the dynamic adaption to nitrogen deficiency. Here, proteomes for 03h, 06h, 12h, 24h, 48h and 10th day of nitrogen deplete and replete conditions were compared. New proteome results were integrated with existing transcriptome and other data.Results Obtained results illuminated physiological adaptations not deduced from previous transcriptome data: (a) Abundance of proteins related to photosynthesis only slightly decreased in the first 48h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48h under nitrogen deficiency, suggesting that respiration is enhanced after 48h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48h, synchronous with the previously reported diversification of fatty acids after 48h.Conclusions This study adds a proteome perspective on the major pathways for TAG accumulation by Nannochloropsis due to absence of nitrogen: photosynthesis, membrane lipid conversion, protein degradation, TCA cycle. By integrating existing transcriptome and other data, our research provided for Nannochloropsis oceanica a multi-layered description of adaptation to nitrogen limitation and lipid accumulation. Cluster analysis of this integrated dataset allowed inference of post-transcriptional regulation events.

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Advances in the biological fixation of carbon dioxide by microalgae

Zhang

Liu

2021

J of Chemical Tech & Biotech

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Global warming caused by carbon dioxide (CO2) emissions is one of the most important topics of concern in the fields of science, environment and global economy. Carbon dioxide emissions have increased dramatically in recent years, and this trend is likely to continue with a projected increase of both global energy consumption and CO2 emissions by 51% in 2050 compared with 2005. To mitigate the negative impacts arising, a potential strategy to convert CO2 into renewable fuels or valuable chemicals can be applied. Bio‐CO2 emission reduction is receiving more and more attention as an environmentally friendly method. Microalgae are attracting worldwide attention in this regard owing to their exceptional speed of growth, resistance to harsh environments and low production costs. In this review, the biochemical principles of microalgae‐CO2 fixation and the research progress of microalgae strains used for CO2 fixation are reviewed and discussed. The latest progress in the co‐development of a microalgae carbon sequestration industry and other industries are provided, along with a survey of the progress in methods to improve microalgae‐CO2 fixation. Finally, the problems to be solved in the industry and its future directions of development are clarified. © 2021 Society of Chemical Industry (SCI).

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Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level

Cited by 65 publications

References 60 publications

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

Advances in the biological fixation of carbon dioxide by microalgae

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