Knockdown of cell division cycle‑associated protein 4 expression inhibits proliferation of triple negative breast cancer MDA‑MB‑231 cells in�vitro and in�vivo

Abstract

Cell division cycle-associated protein 4 (CDCA4), also known as SEI-3/hematopoietic progenitor protein, is a target gene of transcription factor E2F and represses E2F-dependent transcriptional activation and cell proliferation. The present study investigated the effects of CDCA4 knockdown on the regulation of triple negative breast cancer (TNBC) cell proliferation in vitro and in vivo . Human TNBC MDA-MB-231 cells were subjected to CDCA4 expression knockdown using a lentiviral vector carrying CDCA4 or a negative control short hairpin RNA, and reverse transcription-quantitative polymerase chain reaction, MTT cell viability, cell growth, flow cytometric apoptosis, cell cycle and nude mouse tumorigenesis assays were conducted. The knockdown of CDCA4 expression effectively inhibited the growth of MDA-MB-231 cells by promoting apoptosis in vitro . Additionally, CDCA4 expression knockdown suppressed nude mouse tumor cell xenograft formation and growth in vivo . In conclusion, the data from the present study supported the hypothesis that CDCA4 may be involved in regulating human TNBC progression, and that targeting CDCA4 expression could be useful as a novel strategy in future TNBC treatment.

Analysis of the Expression of Cell Division Cycle-Associated Genes and Its Prognostic Significance in Human Lung Carcinoma: A Review of the Literature Databases

“…In a previous study, Hayashi et al showed that CDCA4 participated in cell proliferation [11]. Moreover, CDCA4 is involved in the triple-negative breast cancer (TNBC) cells [36], and it is shown that RNA interference of CDCA4 markedly increases cell apoptotic rate. In addition, one recent study suggests that CDCA4 enhances human BC cell proliferation and reduces their apoptosis [37].…”

Comparison of anadromous and landlocked Atlantic salmon genomes reveals signatures of parallel and relaxed selection across the Northern Hemisphere

“…It is possible that different pathogen or parasite exposure in freshwater and seawater has been a driving force for selection on disease resistance (a topic discussed in more detail by Zueva et al (2018)). Not much is known about cdca4, however, the gene encodes a regulator of transcriptional activation involved in cell proliferation (Hayashi et al, 2006) and has been shown to interact with p53 to promote apoptosis upon DNA damage (Hsieh et al, 2002;Pang et al, 2019). In humans, tRNA copy number variations can have phenotypic effects (Iben & Maraia, 2014;Kirchner & Ignatova, 2015).…”

The diagnostic and prognostic value of cell division cycle associated gene family in Hepatocellular Carcinoma

“…Recently some researchers revealed that CDCAs might contribute to the cancer progression. Previous studies had shown that some members of this gene family might be up-regulated in breast cancer, lung adenocarcinoma and pancreatic adenocarcinoma [5][6][7][8][9][10][11][12][13][14][15][16]. And high expression levels of these genes were identified as poorly predictive factors of survival in these cancers [17,18].…”