Knockdown of double-stranded RNases (dsRNases) enhances oral RNA interference (RNAi) in the corn leafhopper, Dalbulus maidis

Dalaisón-Fuentes, Lucía I.; Pascual, Agustina; Crespo, Mariana; Andrada, Nicolás L.; Welchen, Elina; Catalano, María I.

doi:10.1016/j.pestbp.2023.105618

Cited by 7 publications

(3 citation statements)

References 61 publications

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“…An often-described bottleneck in oral RNAi is the presence of RNases in the insect gut that potentially degrade the iRNAs before they can be taken up by the gut epithelial cells [18,56]. One solution is the co-delivery of iRNA(s) that target the RNase transcripts [64][65][66]. In our experiments, however, the oral delivery of RNase-targeting shRNAs, together with shRNAs against fez2, lrc, or dsx, did not result in a target-specific phenotype in Ae.…”

Section: Discussionmentioning

confidence: 99%

“…A major obstacle to the oral delivery of iRNAs in insects can be the presence of RNases in the gut, which degrade the biologically active RNA molecules before they can be taken up into the gut epithelial cells. One possible solution is the co-delivery of iRNAs targeting the RNase transcripts [64][65][66]. We therefore performed feeding assays with bacteria expressing shRNAs against fez2 or lrc with or without gut RNase-specific shRNAs in two Aedes species, Ae.…”

Section: Co-delivery Of Shrnas Against Gut Rnases Does Not Enhance Ta...mentioning

confidence: 99%

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Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Figueiredo Prates,

Fiebig,

Schlosser

et al. 2024

IJMS

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In this study, we report the complexities and challenges associated with achieving robust RNA interference (RNAi)-mediated gene knockdown in the mosquitoes Aedes aegypti and Aedes albopictus, a pivotal approach for genetic analysis and vector control. Despite RNAi’s potential for species-specific gene targeting, our independent efforts to establish oral delivery of RNAi for identifying genes critical for mosquito development and fitness encountered significant challenges, failing to reproduce previously reported potent RNAi effects. We independently evaluated a range of RNAi-inducing molecules (siRNAs, shRNAs, and dsRNAs) and administration methods (oral delivery, immersion, and microinjection) in three different laboratories. We also tested various mosquito strains and utilized microorganisms for RNA delivery. Our results reveal a pronounced inconsistency in RNAi efficacy, characterized by minimal effects on larval survival and gene expression levels in most instances despite strong published effects for the tested targets. One or multiple factors, including RNase activity in the gut, the cellular internalization and processing of RNA molecules, and the systemic dissemination of the RNAi signal, could be involved in this variability, all of which are barely understood in mosquitoes. The challenges identified in this study highlight the necessity for additional research into the underlying mechanisms of mosquito RNAi to develop more robust RNAi-based methodologies. Our findings emphasize the intricacies of RNAi application in mosquitoes, which present a substantial barrier to its utilization in genetic control strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Co-delivery Of Shrnas Against Gut Rnases Does Not Enhance Ta...mentioning

confidence: 99%

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Figueiredo Prates,

Fiebig,

Schlosser

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…Many studies have reported the use of dsRNA to down-regulate insect genes through feeding. Recently, dsRNA feeding was used for downregulating genes in insects, such as dsRNases of corn leafhopper, Dalbulus maidis ( 23 ) and the glutamate-gated chloride channel gene of the fall armyworm, Spodoptera frugiperda ( 24 ). Further, dsRNA is taken up through an active process involving receptor-mediated endocytosis in Drosophila emanogster S2 cells ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Simulating immunosuppressive mechanism of Microplitis bicoloratus bracovirus coordinately fights Spodoptera frugiperda

Li,

Ma,

Long

et al. 2023

Front. Immunol.

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Parasitoid wasps control pests via a precise attack leading to the death of the pest. However, parasitoid larvae exhibit self-protection strategies against bracovirus-induced reactive oxygen species impairment. This has a detrimental effect on pest control. Here, we report a strategy for simulating Microplitis bicoloratus bracovirus using Mix-T dsRNA targeting 14 genes associated with transcription, translation, cell–cell communication, and humoral signaling pathways in the host, and from wasp extracellular superoxide dismutases. We implemented either one-time feeding to the younger instar larvae or spraying once on the corn leaves, to effectively control the invading pest Spodoptera frugiperda. This highlights the conserved principle of “biological pest control,” as elucidated by the triple interaction of parasitoid-bracovirus-host in a cooperation strategy of bracovirus against its pest host.

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