Knockdown of
            <i>igf3</i>
            gene by RNA interference causes gonadal development inhibition of common carp (
            <i>Cyprinus carpio</i>
            )

Song, Fei; Wang, Lanmei; Yu, Zhenzhen; Zhu, Wenbin; Fu, Jianjun; Dong, Zaijie

doi:10.1111/are.15145

Cited by 4 publications

(1 citation statement)

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“…The transcript abundance of amh was not different in zebrafish exposed to UV‐P. Previous studies have shown that knockdown of expression of the IGF3 gene in common carp ( Cyprinus carpio ) increased concentrations of 11‐KT in blood plasma, increased the proportion of earlier stages of sperm development present in testis, and led to a lesser number of spermatids (Song et al, 2021). This is consistent with the decreased abundance of spermatids and increased concentrations of 11‐KT in UV‐P‐exposed fish, relative to the control, observed in the present study.…”

Section: Discussionmentioning

confidence: 85%

Embryonic Exposure to the Benzotriazole Ultraviolet Stabilizer 2-(2H-benzotriazol-2-yl)-4-methylphenol Decreases Fertility of Adult Zebrafish (Danio rerio)

Raza,

Mertens,

Zink

et al. 2023

Environmental Toxicology and Chemistry

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Benzotriazole ultraviolet stabilizers (BUVSs) are emerging contaminants of concern. They are added to a variety of products including building materials, personal care products, paints, and plastics to prevent degradation caused by UV light. Despite widespread occurrence in aquatic environments, little is known regarding effects of BUVSs on aquatic organisms. The aim of this study was to characterize effects of exposure to 2‐(2H‐Benzotriazol‐2‐yl)‐4‐methylphenol (UV‐P) on reproductive success of zebrafish (Danio rerio) following embryonic exposure. Embryos were exposed, by use of microinjection, to UV‐P at < 1.5 ng/g‐egg (control), 2.77 ng/g‐egg, and 24.25 ng/g egg, and reared until sexual maturity, where reproductive performance was assessed, following which molecular and biochemical endpoints were analyzed. Exposure to UV‐P did not have a significant effect on fecundity. However, there was a significant effect on fertilization success. Using UV‐P exposed males and females, fertility was decreased by 8.75% in the low treatment and by 15.02% in the high treatment, relative to control. In a reproduction assay with UV‐P exposed males and control females, fertility was decreased by 11.47% in the high treatment, relative to the control. Embryonic exposure to UV‐P might have perturbed male sex steroid synthesis as indicated by small changes in blood plasma concentrations of E2 and 11‐KT, and small statistically non‐significant decreases in mRNA abundances of cyp19a1a, cyp11c1, and hsd17b3. Additionally, decreased transcript abundances of genes involved in spermatogenesis, such as nanos2 and dazl, was observed. Decreases in later stages of sperm development were observed, suggesting that embryonic exposure to UV‐P impaired spematogenesis, resulting in decreased sperm quantity. This study is the first to demonstrate latent effects of BUVSs, specifically on fish reproduction.

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Section: Discussionmentioning

confidence: 85%