Knockdown of LASP2 inhibits the proliferation, migration, and invasion of cervical cancer cells

Zhang, Yimeng; Zhang, Liya

doi:10.1002/jcb.28806

Cited by 12 publications

(12 citation statements)

References 21 publications

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“…However, the role of NEBL in cancer is complex. NEBL is a member of nebulin family of actin binding proteins (Zhang and Zhang, 2019). Zhang et.al found that NEBL promoted cancer cell proliferation, migration and invasion in cervical cancer by regulating PI3K/Akt pathway (Zhang and Zhang, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…NEBL is a member of nebulin family of actin binding proteins (Zhang and Zhang, 2019). Zhang et.al found that NEBL promoted cancer cell proliferation, migration and invasion in cervical cancer by regulating PI3K/Akt pathway (Zhang and Zhang, 2019). On the contrary, upregulation of NEBL inhibited cancer cell migration and invasion and reversed TGF-β-induced EMT in prostate cancer (Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

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Gene Signatures and Prognostic Values of N6-Methyladenosine Related Genes in Ovarian Cancer

Fan

Wang

2021

Front. Genet.

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N6-Methyladenosine (m6A) is one of the most prominent modification regulating RNA processing and metabolism. Increasing studies have illuminated the vital role of m6A methylation in carcinogenesis. However, little is known about the interaction between m6A-related genes and survival of ovarian cancer (OC) patients. The purpose of this study was to obtain more reliable m6A-related genes that could be used as prognostic markers of OC using bioinformatics analysis performed on the RNA-seq data of OC. Gene expression datasets of all m6A-related genes as well as corresponding clinical data were obtained from the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA) databases. We detected differential expressed m6A-related candidate genes as well as their relationship and interaction. m6A RNA methylation regulator ALKBH5 and 35 m6A-related genes are dysregulated in OC. A gene set that could be used as a potential independent prognostic risk feature was further screened including NEBL, PDGFRA, WDR91, and ZBTB4. The results of mRNA expression analysis by PCR were consistent with those of bioinformatics analysis. We applied consensus clustering analysis on the expression of the four prognostic genes and obtained four OC subgroups TM1-TM4. There were significant differences in age, stage and grade among the subgroups, and the overall survival (OS) as well as Disease-free survival (DFS) of TM2 group were shorter than those of the other three groups. Further GO and KEGG enrichment analysis indicated that these differential genes were closely related to biological processes and key signaling pathways involved in OC. In summary, our study has indicated that m6A-related genes are key factors in the progression of OC and have potential effects on the prognostic stratification of OC and the development of treatment strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gene Signatures and Prognostic Values of N6-Methyladenosine Related Genes in Ovarian Cancer

Fan

Wang

2021

Front. Genet.

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“…qPCR analysis for the Bcl-2 and DLEU2 genes and miR-15a was performed using an aliquot of first-strand cDNA as the template in a 20- µ l reaction system containing 10 µ l 2X SYBR premixed buffer (Roche, Shanghai, China), 2 µ l forward and reverse primers. The primers were as follows: Bcl-2 forward, 5′-CTGCACCTGACGCCCTTCACC-3′ and reverse, 5′-CACATGACCCCACCGAACTCAAAGA-3′; DLEU2 forward, 5′-TCTGGAGAACAGCCTCACTTC-3 and reverse, 5′-TGCT GAGCTAAGTAGAGGTCTC-3′ ( 18 ); β-actin forward, 5′-CTGGCTCCTAGCACCATGAAGAT-3′ and reverse, 5′-GGTGGACAGTGAGGCCAGGAT-3′ ( 19 ); U6 forward, 5′-CTCGCTTCGGCAGCACATA-3′ and reverse, 5′-CAGTGCAGGGTCCGAGGTA-3′; miR-15a forward, 5′-CGCCTAGCAGCACATAATGG-3′ and reverse, 5′-AGTGCAGGGTCCGAGGTAT-3′ ( 20 ). The PCR amplification process was as follows: 5 min denaturation at 95°C followed by 40 cycles at 95°C for 20 sec, 58°C for 15 sec, and 72°C for 15 sec (ABI StepOnePlus, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

miR‑15a represses cancer cell migration and invasion under conditions of hypoxia by targeting and downregulating Bcl‑2 expression in human osteosarcoma cells

et al. 2018

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Osteosarcoma is a common, high-risk primary bone malignancy that mostly affects the younger population. There has been no marked improvement in the clinical outcomes of osteosarcoma patients to date, and cancer recurrence and metastasis are common in high-grade osteosarcoma. Therefore, identifying new biomarkers and novel therapeutic targets is crucial for improving the prognosis of osteosarcoma patients. In the present study, the MG63 human osteosarcoma cell line was employed to examine the role of microRNA (miR)-15a in regulating cellular activities under hypoxic conditions. It was demonstrated that hypoxia stimulates migration and invasion in MG63 cells, which was correlated with the downregulation of miR-15a and upregulation of B-cell lymphoma 2 (Bcl-2) expression. Introduction of miR-15a or knockdown of endogenous Bcl-2 may reduce hypoxia-induced cell invasion and migration through the regulation of matrix metalloproteinases. Analysis of the expression of miR-15a indicated that hypoxia repressed the transcription of deleted in lymphocytic leukemia 2 (DLEU2), which is the host gene of miR-15a. These findings indicated that miR-15a may be a valuable target for the treatment of osteosarcoma, particularly for patients with high-grade cancer or heavy tumor burden.

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“…In addition, SRC tyrosine kinase is closely related to tumor cell growth, metastasis, cell signal transduction and angiogenesis. This target has been recognized as an important anti-tumor target [48], and is also a research hotspot of tumor treatment [49,50]. Previous studies [51][52][53] have confirmed that Src kinase inhibitor PP2 can inhibit the proliferation, migration and invasion of lung cancer cells by inhibiting the activity of the enzyme.…”

Section: Discussionmentioning

confidence: 99%

Identification of Functional Gene Modules and Biomarkers of Apatinib Against Lung Adenocarcinoma Based on Weighted Gene Co-expression Network Ansalysis (WGCNA)

Luo¹,

Zhong²,

Guosong³

et al. 2021

SJCM

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Objective: Apatinib is a drug for the treatment of gastric cancer. In recent years, studies have found that it also has good efficacy in the treatment of lung adenocarcinoma, but its mechanism of action is still not clear. Therefore, this study uses bioinformatics and Experimental verification explores the mechanism of apatinib in the treatment of lung adenocarcinoma. To explore the potential biological targets of apatinib against lung adenocarcinoma based on weighted gene co-expression network analysis (WGCNA). Methods: The PharmMapper server was first used to reversely predict the potential targets of apatinib. Subsequently, it was combined with WGCNA to mine the GSE10072 data set in the GEO database of the National Center for Biotechnology Information (NCBI) to obtain the co-expressed gene module. Next, it was combined with apatinib Predict the target matching mapping to obtain the potential anti-lung adenocarcinoma target of apatinib. The STRING database was combined with Cytoscape software to visualize the protein interaction network of apatinib's potential anti-lung adenocarcinoma target protein and perform network topology analysis, as well as to obtain the core target from the network. Kaplan Meier plotter database was applied to analyze the relationship between key genes and the prognosis of patients with lung adenocarcinoma. Molecular docking technology was used for the potential of apatinib. Anti-lung adenocarcinoma target protein was finally verified by molecular interaction. Finally, Western blot was employed to analyze the expression level of the key target protein. Results: A total of 300 targets of apatinib were obtained, and 45 potential targets of apatinib against lung adenocarcinoma were screened. From the protein interaction network analysis, the key genes included CASP3, EGFR, MMP9, SRC, CASP8, CASP9, STAT3 and MAPK1. High expression of EGFR, SRC, MAPK1 and STAT3, and low expression of CASP3, CASP8, CASP9 and MMP9 were closely related to poor prognosis of patients with lung adenocarcinoma. Molecular docking showed that the interaction of apatinib with the targets of MAPK1, CASP3, EGFR, SRC, MMP9 and STAT3 was comparable to the positive control. Western blot showed that with the increasing of drug concentration, STAT3 were down-regulated, and CASP8 were up-regulated. Conclusion: The mechanism of apatinib in the treatment of lung adenocarcinoma is mainly through the regulation of the signal transduction pathway and the apoptosis pathway, which provides a scientific basis for the study of its anti-lung adenocarcinoma mechanism.

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Knockdown of LASP2 inhibits the proliferation, migration, and invasion of cervical cancer cells

Cited by 12 publications

References 21 publications

Gene Signatures and Prognostic Values of N6-Methyladenosine Related Genes in Ovarian Cancer

Gene Signatures and Prognostic Values of N6-Methyladenosine Related Genes in Ovarian Cancer

miR‑15a represses cancer cell migration and invasion under conditions of hypoxia by targeting and downregulating Bcl‑2 expression in human osteosarcoma cells

Identification of Functional Gene Modules and Biomarkers of Apatinib Against Lung Adenocarcinoma Based on Weighted Gene Co-expression Network Ansalysis (WGCNA)

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