Knockdown of LINC01385 inhibits osteoarthritis progression by modulating the microRNA‑140‑3p/TLR4 axis

Wang, Zidong; Huang, Chuanwang; Zhao, Cunju; Zhang, Huiling; Zhen, Zhen; Xu, Duliang

doi:10.3892/etm.2021.10679

Cited by 11 publications

(7 citation statements)

References 31 publications

(55 reference statements)

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“…To moreover check on the circKMT2E/miR-140-5p/TLR4 regulatory pathway, our research team performed a dual-luciferase reporter assessment to confirm the combinative connection between circKMT2E and miR-140-5p, they used transfected cells to verify the ability of circKMT2E to promote apoptosis. Wang et al [ 21 ] previously found that knockdown of LINC01385 can inhibit the progression of OA on the basis of adjusting the microRNA-140-3p/TLR4 axis. Thus, it can be deduced that upregulated circKMT2E in chondrocytes with OA may be involved in the apoptosis of chondrocytes by targeting TLR4 via the sponge function to miR-140-5p.…”

Section: Discussionmentioning

confidence: 99%

CircKMT2E Participates in Osteoarthritis through Promotes Apoptosis of Chondrocytes Via Sponging miR-140-5p to Activate TLR4

Zhou

Cheng

2022

Evidence-Based Complementary and Alternative Medicine

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Objective To explore the latent pathogenesis of circRNAs in osteoarthritis (OA), as well as their function mechanism. Methods The murine chondrocytes with and without OA were involved and used for in-depth sequencing. Herein, we carried out subsequent bioinformatics analysis to disclose the expression pattern, characteristics of circRNAs based on gene ontology, and the KEGG pathway analyses. Then sequencing data were used to deduce the interaction between circRNA and miRNA. The potential miRNA response elements for the annotated circRNAs and relevant target genes were forecasted on the basis of TargetScan and miRanda. For chondrocytes, the effect of the overexpression of the screened circRNA for apoptosis was spotted by flow cytometry as well as Western Blot. Results 466 diverse circRNAs in the 23,787 spotted circRNAs were both significantly and differentially transcribed. CircKMT2E was upregulated more than two folds in chondrocytes with OA compared with normal tissues, exhibiting an expression trend opposite to miR-140-5p. We disclosed that circKMT2E could possess mutual effect with miR-140-5p by way of AGO proteins. Thus, circKMT2E was verified to have functioned as a molecular sponge targeting miR-140-5p. Therefore, circKMT2E may be at work in the pathogenesis of OA. Further, the sponge connection between circKMT2E and miR-140-5p was proved on the basis of a dual-luciferase reporter assay. Besides, miR-140-5p was speculated can bind TLR4 by bioinformatics analysis. Further PCR analysis found the relative expression level of TLR4, caspase-3, and Bax in the OA groups presented significant upregulation. Overexpression of circKMT2E can promote apoptosis of chondrocytes. Conclusion The upregulation of circKMT2E is involved in the chondrocyte apoptosis of the pathogenesis of OA through activation of TLR4 by the sponge function of miR-140-5p.

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Section: Discussionmentioning

confidence: 99%

CircKMT2E Participates in Osteoarthritis through Promotes Apoptosis of Chondrocytes Via Sponging miR-140-5p to Activate TLR4

Zhou

Cheng

2022

Evidence-Based Complementary and Alternative Medicine

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“…In both tuberculosis and carciogenesis, Il‐6 play an important role again [68, 69]. Regarding the miR‐140‐3p, it is evident that this miRNA is also significantly down‐regulated in human chondrocytes in glenohumeral arthritis [70], among others. MiR‐140‐3p was shown to reduce the concentration of interleukin‐1ß (IL‐1ß) induced inflammatory factors [70].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the miR‐140‐3p, it is evident that this miRNA is also significantly down‐regulated in human chondrocytes in glenohumeral arthritis [70], among others. MiR‐140‐3p was shown to reduce the concentration of interleukin‐1ß (IL‐1ß) induced inflammatory factors [70]. IL1‐ß plays a crucial role mainly in the inflammatory phase of tendon healing [71], but it has also been shown that it has a significant role in arthritis [72].…”

Section: Discussionmentioning

confidence: 99%

Moderate evidence exists for four microRNAs as potential biomarkers for tendinopathies and degenerative tendon ruptures at the upper extremity in elderly patients: conclusion of a systematic review with best‐evidence synthesis

Schmid

Wegener

Hotfiel³

et al. 2023

J. exp. orthop.

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Purpose The aim of this systematic review was to investigate tendon-specific microRNAs (miRNAs) as biomarkers for the detection of tendinopathies or degenerative tendon ruptures. Also, their regulatory mechanisms within the tendon pathophysiology were summarized. Methods A systematic literature research was performed using the PRISMA guidelines. The search was conducted in the Pubmed database. The SIGN checklist was used to assess the study quality of the included original studies. To determine the evidence and direction of the miRNA expression rates, a best-evidence synthesis was carried out, whereby only studies with at least a borderline methodological quality were considered for validity purposes. Results Three thousand three hundred seventy studies were reviewed from which 22 fulfilled the inclusion criteria. Moderate evidence was found for miR-140-3p and miR-425-5p as potential biomarkers for tendinopathies as well as for miR-25-3p, miR-29a-3p, miR-140-3p, and miR-425-5p for the detection of degenerative tendon ruptures. This evidence applies to tendons at the upper extremity in elderly patients. All miRNAs were associated with inflammatory cytokines as interleukin-6 or interleukin-1ß and tumor necrosis factor alpha. Conclusions Moderate evidence exists for four miRNAs as potential biomarkers for tendinopathies and degenerative tendon ruptures at the upper extremity in elderly patients. The identified miRNAs are associated with inflammatory processes.

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“…Circular RNA S-phase cyclin A associated protein in the endoplasmic reticulum (circSCAPER) is highly expressed in IL-1β-treated chondrocytes and OA cartilage and has been reported to act as a “sponge” of miR-140-3p ( 78 ). miR-140 was also shown to be the target of long non-coding RNAs LINC01385 ( 79 ) and plasmacytoma variant translocation 1 (PVT1) ( 80 ). Restoration of miR-140 is being tested as a therapeutic approach in preclinical models of OA.…”

Section: Osteoarthritismentioning

confidence: 99%

Challenges and promise of targeting miRNA in rheumatic diseases: a computational approach to identify miRNA association with cell types, cytokines, and disease mechanisms

Shaikh,

Siegel,

Srivastava

et al. 2024

Front. Immunol.

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MicroRNAs (miRNAs) are small non-coding RNAs that alter the expression of target genes at the post-transcriptional level, influencing diverse outcomes in metabolism, cell differentiation, proliferation, cell survival, and cell death. Dysregulated miRNA expression is implicated in various rheumatic conditions, including ankylosing spondylitis (AS), gout, juvenile idiopathic arthritis (JIA), osteoarthritis (OA), psoriatic arthritis, rheumatoid arthritis (RA), Sjogren’s syndrome, systemic lupus erythematosus (SLE) and systemic sclerosis. For this review, we used an open-source programming language- PowerShell, to scan the massive number of existing primary research publications on PubMed on miRNAs in these nine diseases to identify and count unique co-occurrences of individual miRNAs and the disease name. These counts were used to rank the top seven most relevant immuno-miRs based on their research volume in each rheumatic disease. Individual miRNAs were also screened for publication with the names of immune cells, cytokines, and pathological processes involved in rheumatic diseases. These occurrences were tabulated into matrices to identify hotspots for research relevance. Based on this information, we summarize the basic and clinical findings for the top three miRNAs — miR-146, miR-155, and miR-21 — whose relevance spans across multiple rheumatic diseases. Furthermore, we highlight some unique miRNAs for each disease and why some rheumatic conditions lack research in this emerging epigenetics field. With the overwhelming number of publications on miRNAs in rheumatic diseases, this review serves as a ‘relevance finder’ to guide researchers in selecting miRNAs based on the compiled existing knowledge of their involvement in disease pathogenesis. This approach applies to other disease contexts with the end goal of developing miRNA-based therapeutics.

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Knockdown of LINC01385 inhibits osteoarthritis progression by modulating the microRNA‑140‑3p/TLR4 axis

Cited by 11 publications

References 31 publications

CircKMT2E Participates in Osteoarthritis through Promotes Apoptosis of Chondrocytes Via Sponging miR-140-5p to Activate TLR4

CircKMT2E Participates in Osteoarthritis through Promotes Apoptosis of Chondrocytes Via Sponging miR-140-5p to Activate TLR4

Moderate evidence exists for four microRNAs as potential biomarkers for tendinopathies and degenerative tendon ruptures at the upper extremity in elderly patients: conclusion of a systematic review with best‐evidence synthesis

Challenges and promise of targeting miRNA in rheumatic diseases: a computational approach to identify miRNA association with cell types, cytokines, and disease mechanisms

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