Knockdown of lncRNA ENST00000609755.1 Confers Protection Against Early oxLDL-Induced Coronary Heart Disease

Abstract

Background: This study investigated the association between long non-coding RNAs (lncRNAs) and coronary heart disease (CHD) and further elucidated the potential biological roles of lncRNAs in CHD pathogenesis.Methods: A case-control study (590 patients and 590 controls) was conducted from February 2017 and March 2019 in Fuzhou, China. Environmental factors were investigated using questionnaires and physical examinations. Five representative lncRNAs were screened using lncRNA microarray (peripheral blood in 5 cases and 5 controls) and further verified by quantitative real-time polymerase chain reaction (peripheral blood leukocyte in 100 cases and 100 controls). Oxidized low-density lipoprotein (oxLDL) was used to induce a human coronary artery endothelial cell (HCAECs) injury model, and loss of function was used to elucidate the role of lncRNA ENST00000609755.1 (lnc-MICALL2-2) in oxLDL-induced HCAECs injury.Results: A total of 320 lncRNAs were found dysregulated in CHD patients (fold change> 2, p < 0.05). The results of a discovery microarray, population verification and HCAEC experiments suggested the lnc-MICALL2-2 is upregulated in CHD subjects and in an oxLDL-induced HCAECs injury model. Conversely, lnc-MICALL2-2 inhibition in vitro attenuated the effects of oxLDL on HCAECs morphology, proliferation, and apoptosis.Conclusion: Elevated expression of lnc-MICALL2-2 is an independent risk factor for CHD, and knockdown subsequently confers protection against early pathological processes of oxLDL-induced CHD.

Effect of berberine on global modulation of lncRNAs and mRNAs expression profiles in patients with stable coronary heart disease

“…Sun et al found that lnc-MICALL2-2 is up-regulated in CHD subjects. Knockdown lnc-MICALL2-2 subsequently confers protection against early pathological processes of oxidized low-density lipoprotein-induced CHD [ 10 ]. The level of lncRNA H19 is significantly decreased in the heart tissue of Sprague-Dawley rats undergoing MI surgery.…”

Expression of lncRNA APF in Peripheral Blood of Patients with Acute Myocardial Infarction Caused by Coronary Heart Disease and its Clinical Significance

“…In addition to environmental and genetic factors, there is growing evidence that epigenetic changes may play a key role in the development of CHD. 8) Therefore, it has become a new challenge to further understand the pathogenesis of CHD and to search for markers for early diagnosis of CHD.…”

“…11) Many of these lncRNAs play a role in pluripotency regulation and activation of heart-specific genes in particular and have been shown to be associated with CHD risk and AMI. 8) It has been reported that lncRNAs are present in the blood and may serve as diagnostic markers for cardiovascular diseases. 12) However, no specific lncRNA has been found that can be used to effectively identify early CHD.…”