Knockdown of MCM10 Gene Impairs Glioblastoma Cell Proliferation, Migration and Invasion and the Implications for the Regulation of Tumorigenesis

Peng, Kang; Han, Zhe; Liao, Zewen; Zhang, Heng; Jia, Wang; Tian, Yongji

doi:10.1007/s12031-020-01486-y

Cited by 9 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Next, a set of abnormal signaling pathways within cells serves as the second hit to contribute to further disease progression. For example, in this study, UBASH3A, SF3B1, RUNX1, ERBB4 and MCM10 are associated with NF-κB, Ras-MAPK-ERK, P13K-Akt and Wnt/β-catenin pathway abnormalities [51][52][53][54][55][56][57][58][59]. Activation of these signaling pathways can lead to accelerated cell proliferation, followed by an increased number of cells carrying genetic mutations.…”

Section: Discussionmentioning

confidence: 57%

Polyclonal evolution of Fanconi anemia to MDS and AML revealed at single cell resolution

et al. 2022

View full text Add to dashboard Cite

Background Fanconi anemia (FA) is a rare disease of bone marrow failure. FA patients are prone to develop myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). However, the molecular clonal evolution of the progression from FA to MDS/AML remains elusive. Methods Herein, we performed a comprehensive genomic analysis using an FA patient (P1001) sample that transformed to MDS and subsequently AML, together with other three FA patient samples at the MDS stage. Results Our finding showed the existence of polyclonal pattern in these cases at MDS stage. The clonal evolution analysis of FA case (P1001) showed the mutations of UBASH3A, SF3B1, RUNX1 and ASXL1 gradually appeared at the later stage of MDS, while the IDH2 alteration become the dominant clone at the leukemia stage. Moreover, single-cell sequencing analyses further demonstrated a polyclonal pattern was present at either MDS or AML stages, whereas IDH2 mutated cell clones appeared only at the leukemia stage. Conclusions We thus propose a clonal evolution model from FA to MDS and AML for this patient. The results of our study on the clonal evolution and mutated genes of the progression of FA to AML are conducive to understanding the progression of the disease that still perplexes us.

show abstract

Section: Discussionmentioning

confidence: 57%

Polyclonal evolution of Fanconi anemia to MDS and AML revealed at single cell resolution

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Several of the differentially expressed genes in both CRISPRi and CRISPRa are direct or indirect MYC responsive genes, including MCM10, CDCA7, TP53, CDKN2A, CCNF , and CDK8 (Yang et al, 2018, Prescott et al, 2001, Koch et al, 2007, Kang et al, 2020, Gill et al, 2013, Kidder et al, 2008, Phesse et al, 2014, Garcia-Gutierrez et al, 2019). SNHG26 was detected as a MYC-target in P493-6 human B-cells, where it was upregulated in response to MYC overexpression (Hart et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, MCM10 is a CDK substrate and is associated with chromatin in the late stage of replication initiation, although it is not necessary for the process (Chadha et al, 2016). MCM10 is often overexpressed in several cancers, and its expression is associated with tumor progression (Kang et al, 2020, Cui et al, 2018, Baxley and Bielinsky, 2017)). Moreover, MCM10 is listed as one of the top ranked cancer-associated genes in non-small-cell lung cancer (NSCLC) and breast cancer across different methods and from independent patient datasets (Wu et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

The G2-phase enriched lncRNASNHG26is necessary for proper cell cycle progression and proliferation

Samdal

Hegre

Chawla

et al. 2021

Preprint

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) are involved in the regulation of cell cycle, although only a few have been functionally characterized. By combining RNA sequencing and ChIP sequencing of cell cycle synchronized HaCaT cells we have previously identified lncRNAs highly enriched for cell cycle functions. Based on a cyclic expression profile and an overall high correlation to histone 3 lysine 4 trimethylation (H3K4me3) and RNA polymerase II (Pol II) signals, the lncRNA SNHG26 was identified as a top candidate. In the present study we report that downregulation of SNHG26 affects mitochondrial stress, proliferation, cell cycle phase distribution, and gene expression in cis- and in trans, and that this effect is reversed by upregulation of SNHG26. We also find that the effect on cell cycle phase distribution is cell type specific and stable over time. Results indicate an oncogenic role of SNHG26, possibly by affecting cell cycle progression through the regulation of downstream MYC-responsive genes.

show abstract

“…MCM10 is an essential protein in DNA replication by forming complexes with MCM2–7 , and it participates in multiple molecular and cellular processes, such as DNA replication, early embryogenesis, and normal embryonic development. MCM10 is also involved in the formation and development of multiple tumors, including glioma, prostate cancer, urothelial cancer, neuroblastoma, and breast cancer ( 48 ). Together with MCM7 , the upregulation of MCM10 expression was associated with an increase of NK cells ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Minichromosome Maintenance Proteins and Their Prognostic Significance in Melanoma

Han

Zhao

et al. 2021

Front. Oncol.

View full text Add to dashboard Cite

BackgroundMinichromosome maintenance (MCM) is known for participating in cell cycle progression, as well as DNA replication. While the diverse expression patterns and prognostic values of MCMs in melanoma still remained unclear.MethodsIn the present study, the transcriptional and clinical profiles of MCMs were explored in patients with melanoma from multiple databases, including GEO, TCGA, ONCOMINE, GEPIA, UALCAN, cBioPortal, and TIMER databases.ResultsWe found that the elevated expressions of MCM2–6 and MCM10 were significantly expressed in melanoma compared to normal skin. High mRNA levels of MCM4, MCM5, and MCM10 were closely related to worse prognosis in patients with melanoma. GSEA showed hallmark pathways were most involved in mTORC1 signaling, G2M checkpoint, E2F targets, and mitotic spindle. Furthermore, we found potential correlations between the MCM expression and the immune cell infiltration, including B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cells.ConclusionUpregulated MCM gene expression in melanoma probably played a crucial part in the development and progression of melanoma. The upregulated MCM4/5/10 expressions could be used as potential prognostic markers to improve the poor outcome and prognostic accuracy in patients with melanoma. Our study might shed light on the selection of prognostic biomarkers as well as the underlying molecular pathogenesis of melanoma.

show abstract

Knockdown of MCM10 Gene Impairs Glioblastoma Cell Proliferation, Migration and Invasion and the Implications for the Regulation of Tumorigenesis

Cited by 9 publications

References 34 publications

Polyclonal evolution of Fanconi anemia to MDS and AML revealed at single cell resolution

Polyclonal evolution of Fanconi anemia to MDS and AML revealed at single cell resolution

The G2-phase enriched lncRNASNHG26is necessary for proper cell cycle progression and proliferation

Integrative Analysis of Minichromosome Maintenance Proteins and Their Prognostic Significance in Melanoma

Contact Info

Product

Resources

About