Knockdown of NAA25 Suppresses Breast Cancer Progression by Regulating Apoptosis and Cell Cycle

Abstract

NAA25 gene variants were reported as risk factors for type 1 diabetes, rheumatoid arthritis and acute arterial stroke. But it’s unknown whether it could contribute to breast cancer. We identified rs11066150 in lncHSAT164, which contributes to breast cancer, in our earlier genome-wide long non-coding RNA association study on Han Chinese women. However, rs11066150 A/G variant is also located in NAA25 intron. Based on the public database, such as TCGA and Curtis dataset, NAA25 gene is highly expressed in breast cancer tissues and this result has also been proved in our samples and cell lines through RT-qPCR and western blot analysis. To better understand the function of NAA25 in breast cancer, we knocked down the expression of NAA25 in breast cancer cell lines, FACS was used to detect cell apoptosis and cell cycle and colony formation assay was used to detect cell proliferation. We found that NAA25-deficient cells could increase cell apoptosis, delay G2/M phase cell and decrease cell clone formation. RNA sequencing was then applied to analyze the molecular profiles of NAA25−deficient cells, and compared to the control group, NAA25 knockdown could activate apoptosis-related pathways, reduce the activation of tumor-associated signaling pathways and decrease immune response-associated pathways. Additionally, RT-qPCR was employed to validate these results. Taken together, our results revealed that NAA25 was highly expressed in breast cancer, and NAA25 knockdown might serve as a therapeutic target in breast cancer.

Novel biallelic variants expand the phenotype of NAA20‐related syndrome

“…Previously reports have linked NAA20 with neuronal morphology and function in drosophila, 18,19 while several other findings suggested a pro‐oncogenic role of both NAA20 and NAA25 20,21 . Conversely, there is little data regarding the possible role of Nt‐acetylation in prenatal development.…”

“…6,[12][13][14][15][16][17] Previously reports have linked NAA20 with neuronal morphology and function in drosophila, 18,19 while several other findings suggested a pro-oncogenic role of both NAA20 and NAA25. 20,21 Conversely, there is little data regarding the possible role of Nt-acetylation in prenatal development. We may speculate that NAA20 and NAA25 are highly expressed during nervous system development similar to NAA10 and NAA15.…”

Morphological and molecular evolution of hadal amphipod’s eggs provides insights into embryogenesis under high hydrostatic pressure

“…In Drosophila , Psidin/Naa25 is essential for neuron survival and axon targeting ( Stephan et al, 2012 ). Naa25 -deficient cells would increase cell apoptosis ( Xu et al, 2021 ). Moreover, the mRNA expression of Drosophila nicotinamidase (D- NAAM ) was found to be up-regulated by the low oxidative stress, which might protect the neuronal cells from oxidative stress-induced cell death ( Balan et al, 2008 ).…”