Knockdown of Rice MicroRNA166 Confers Drought Resistance by Causing Leaf Rolling and Altering Stem Xylem Development

Zhang, Jinshan; Zhang, Hui; Srivastava, Ashish Kumar; Pan, Yujie; Bai, Jinjuan; Fang, Jingjing; Shi, Huazhong; Zhu, Jian‐Kang

doi:10.1104/pp.17.01432

Cited by 226 publications

(156 citation statements)

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“…dlo-miR397a, dlo-miR408-3p and dlo-miR166a-3p might participate in the development of early SE via the lignin metabolism pathway. miR397, miR408 and miR166 are conserved miRNAs in plants, which have been reported to participate in lignin and xylem synthesis [51][52][53] . Laccase and HD-Zip have also been widely studied [54][55][56] .…”

Section: Many Mirnas Are Involved In Early Se In Longanmentioning

confidence: 99%

Genome-wide identification of miRNAs and their targets during early somatic embryogenesis in Dimocarpus longan Lour.

Chen

et al. 2020

Sci Rep

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miRNAs are endogenous regulatory factors that play pivotal roles in post-transcriptional regulation. However, their specific roles in early somatic embryogenesis (SE) remain unclear. Study of the SE system is fundamental for clarifying the molecular mechanisms in Dimocarpus longan. We identified 289 known miRNAs from 106 different miRNA families and 1087 novel miRNAs during early longan SE, including embryogenic callus (EC), incomplete pro-embryogenic culture (ICpEC), globular embryo (GE), and non-embryogenic callus (NEC). The abundances of known miRNAs were concentrated in GE. The differentially expression (DE) miRNAs showed five expression patterns during early SE. Largely miRNAs were expressed highly and specially in EC, ICpEC, and GE, respectively. Some miRNAs and putative target genes were enriched in lignin metabolism. Most potential targets were related to the pathways of plant hormone signal transduction, alternative splicing, tyrosine metabolism and sulfur metabolism in early longan SE. The regulatory relationships between dlo-miR166a-3p and DlHD-zip8, dlo-miR397a and DlLAC7, dlo-miR408-3p and DlLAC12 were confirmed by RNA ligase-mediated rapid amplification of cDNA ends. The expression patterns of eight DE miRNAs detected by qRT-PCR were consistent with RNA-seq. Finally, the miRNA regulatory network in early SE was constructed, which provided new insight into molecular mechanism of early SE in longan.Plant somatic embryogenesis (SE) is physiologically and morphologically similar to that of zygotic embryos. Therefore, SE is usually used as a model system for studying embryonic development in higher plants. Previously study demonstrated that transcription factors (TFs) regulated embryogenic transformation in SE 1 ; this TFs included BABY BOOM (BBM), AGAMOUS-LIKE15 (AGL15), LEC2 and SERK, which functioned in the process of cell division and differentiation in embryonic development 2 . miRNAs are a kind of small endogenous RNAs of 18-24nt in length, which mainly recognize the complementary binding sites of their target mRNAs. miRNAs were transcribed by RNA polymerase II (Pol II). miRNAs could mediate mRNA cleavage and inhibiting translation at transcriptional or post-transcriptional level in eukaryotes 3,4 . Over the past decade, miRNAs have been reported to be involved in a broad range of metabolic and physiological processes in plants, such as plant growth, development and responses to stresses. However, the regulation of miRNA of plant SE was still unclear.The miRNA regulatory pathway is particularly important in plant growth and development, hormone regulation, organ differentiation, alternative splicing and secondary metabolite accumulation 5-7 . The SE could be influenced by epigenetics in some plant 8 . For instance, expression levels of miR171, 159, 169 and 172 affected in the embryogenic potency of Larix gmelini 9 , these particular miRNAs targeted to multifunctional TFs and participated in ABA signal transduction, growth and development, and stress response. In Citrus sinensis (L.), miR156, 168 an...

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Section: Many Mirnas Are Involved In Early Se In Longanmentioning

confidence: 99%

Genome-wide identification of miRNAs and their targets during early somatic embryogenesis in Dimocarpus longan Lour.

Chen

et al. 2020

Sci Rep

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“…STTM induces the degradation of most, if not all, cognate miRNAs, partly through the action of small degrading nucleases (Yan et al, 2012). STTMs have been successfully applied to silence numerous miRNA families in Arabidopsis (Yan et al, 2012) and in a few staple crops and model plants, including tomato (Jia et al, 2015;Jiang et al, 2018), rice (Zhang et al, 2017a(Zhang et al, , 2018, wheat (Guo et al, 2018), tobacco (Sha et al, 2014), Medicago (Proust et al, 2018), soybean (Wong et al, 2014;Bao et al, 2018), poplar (Su et al, 2018), cotton (Gu et al, 2014), common bean (Sosa-Valencia et al, 2017), and barley (Liu et al, 2014), and even in animals (Liu et al, 2018). STTMs have been introduced into plant cells through stable transformation (Yan et al, 2012), Agrobacterium-mediated transient expression (Zhang et al, 2017b), and virus-inducedgene silencing (Sha et al, 2014;Jian et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants

Peng

Qiao

et al. 2018

Molecular Plant

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microRNAs (miRNAs) are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression, leading to gene silencing. We previously developed short tandem target mimic (STTM) technology to deactivate endogenous miRNAs in Arabidopsis. Here, we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis, tomato, rice, and maize, providing a resource for the functional interrogation of miRNAs. We not only revealed the functions of several miRNAs in plant development, but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and development. RNA-seq and small RNA-seq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation, secondary metabolism, and ion-channel activity-associated electrophysiology, demonstrating that STTM technology is an effective approach for studying miRNA functions. To facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks, we have established an online Genome Browser (https://blossom.ffr.mtu.edu/designindex2.php) to display the transcriptomic and miRNAomic changes in STTM-induced miRNA knockdown plants.

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“…that miR166 also regulates plant responses to abiotic stresses, including drought in Triticum dicoccoides and rice (Oryza sativa; Kantar et al, 2011;Zhang et al, 2018) and mercury treatment in Medicago truncatula (Zhou et al, 2012). Our previous work from microarray assays showed that miR166 was strongly down-regulated in rice by Cd stress (Ding et al, 2011).…”

mentioning

confidence: 98%

MicroRNA166 Modulates Cadmium Tolerance and Accumulation in Rice

et al. 2018

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MicroRNAs (miRNAs) are 20- to 24-nucleotide small noncoding RNAs that regulate gene expression in eukaryotic organisms. Several plant miRNAs, such as miR166, have vital roles in plant growth, development and responses to environmental stresses. One such environmental stress encountered by crop plants is exposure to cadmium (Cd), an element highly toxic to most organisms, including humans and plants. In this study, we analyzed the role of miR166 in Cd accumulation and tolerance in rice (). The expression levels of miR166 in both root and leaf tissues were significantly higher in the reproductive stage than in the seedling stage in rice. The expression of miR166 in the roots of rice seedlings was reduced after Cd treatment. Overexpression of miR166 in rice improved Cd tolerance, a result associated with the reduction of Cd-induced oxidative stress in transgenic rice plants. Furthermore, overexpression of miR166 reduced both Cd translocation from roots to shoots and Cd accumulation in the grains. miR166 targets genes encoding the class-III homeodomain-Leu zipper (HD-Zip) family proteins in plants. In rice, () gene (Os03g43930), which encodes an HD-Zip protein, was up-regulated by Cd treatment but down-regulated by overexpression of miR166 in transgenic rice plants. Overexpression of increased Cd sensitivity and Cd accumulation in the leaves and grains of transgenic rice plants. By contrast, silencing by RNA interference enhanced Cd tolerance in transgenic rice plants. These results indicate a critical role for miR166 in Cd accumulation and tolerance through regulation of its target gene, , in rice.

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Knockdown of Rice MicroRNA166 Confers Drought Resistance by Causing Leaf Rolling and Altering Stem Xylem Development

Cited by 226 publications

References 60 publications

Genome-wide identification of miRNAs and their targets during early somatic embryogenesis in Dimocarpus longan Lour.

Genome-wide identification of miRNAs and their targets during early somatic embryogenesis in Dimocarpus longan Lour.

A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants

MicroRNA166 Modulates Cadmium Tolerance and Accumulation in Rice

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