Knockdown of SIRT7 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the Wnt/β-catenin signaling pathway

Abstract

Sirtuin 7 (SIRT7) is a NAD+-dependent deacetylase in the sirtuin family. In a previous study, human bone marrow mesenchymal stem cells (hBMSCs) with reduced SIRT7 activity were developed to evaluate the effect of SIRT7 on osteogenesis. SIRT7 knockdown significantly enhanced osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. Additionally, SIRT7 knockdown upregulated β-catenin. The enhanced osteogenesis due to SIRT7 knockdown was partially rescued by a Wnt/β-catenin inhibitor. Furthermore, SIRT7 knockdown hBMSCs combined with a chitosan scaffold significantly promoted bone formation in a rat tibial defect model, as determined by imaging and histological examinations. These findings suggest that SIRT7 has an essential role in osteogenic differentiation of hBMSCs, partly by activation of the Wnt/β-catenin signaling pathway.

Exosomal miR‐130a‐3p regulates osteogenic differentiation of Human Adipose‐Derived stem cells through mediating SIRT7/Wnt/β‐catenin axis

“…34 It is reported that several members of the sirtuin family are closely related to Wnt signalling pathway. 20,35,36 In this study, we found that overexpression of miR-130a-3p can decrease SIRT7 expression while increasing β-cat- More and more studies have confirmed that exosome plays an important role in osteogenesis. 37,38 In our present study, for the first time, we confirmed the effect of exosomal miR-130a-3p on proliferation and osteogenic differentiation of ADSCs.…”

Concentration‐dependent, dual roles of IL‐10 in the osteogenesis of human BMSCsviaP38/MAPK and NF‐κB signaling pathways

“…The results of immunofluorescence analysis of RUNX2, a master transcription factor in osteogenic differentiation (47), and COL1A1 were consistent with the observations from Western blot analysis. ALP staining and calcium deposits are regarded as early and late markers, respectively, of osteoblastic differentiation (48). We found that IL-10 enhanced ALP activity and accelerated mineralization at lower concentrations and vice versa.…”

Knockdown of FOXA2 enhances the osteogenic differentiation of bone marrow-derived mesenchymal stem cells partly via activation of the ERK signalling pathway

“…Briefly, rats were anaesthetised intraperitoneally with 0.3% pentobarbital sodium (Sigma) at 30 mg/kg body weight. After anaesthesia, an incision was made below the knee, which was followed by an intramedullary needle (1.2 mm-diameter stainless steel syringe needle) being inserted inside the medullary canal of the tibia for fixation 52 . A 1.5 mm-diameter tibial defect was made in all SD rats approximately 1 cm from the proximal tibial growth plate by a hollow drill 51 – 53 .…”

“…Serial sections (3 µm thickness) were cut and mounted onto polylysine-coated glass slides, and deparaffinized. Sections were stained with HE, Safranin O/Fast Green and Masson’s trichrome staining were performed separately consecutive tissue sections in accordance with our previous studies 48 , 52 . Images were obtained on a traditional light microscopy (Leica DM4000B; Leica, Solms, Germany).…”