Knockdown of the Adipokinetic Hormone Receptor Increases Feeding Frequency in the Two-Spotted Cricket Gryllus bimaculatus

Konuma, Takahiro; Morooka, Nobukatsu; Nagasawa, Hiromichi; Nagata, Shinji

doi:10.1210/en.2011-1533

Cited by 57 publications

(69 citation statements)

References 44 publications

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“…If AKH influences nutrient level changes in the fat body and hemolymph, it might additionally influence feeding behavior. Consistent with that hypothesis, knockdown of an AKH receptor in the fat body of the cricket Gryllus bimaculatus resulted in decreased circulating sugar levels, and increased feeding frequency and overall food consumption [94].…”

Section: Resultssupporting

confidence: 66%

Identification and Characterization of Seminal Fluid Proteins in the Asian Tiger Mosquito, Aedes albopictus

et al. 2014

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The Asian tiger mosquito (Aedes albopictus) is an important vector for pathogens that affect human health, including the viruses that cause dengue and Chikungunya fevers. It is also one of the world's fastest-spreading invasive species. For these reasons, it is crucial to identify strategies for controlling the reproduction and spread of this mosquito. During mating, seminal fluid proteins (Sfps) are transferred from male mosquitoes to females, and these Sfps modulate female behavior and physiology in ways that influence reproduction. Despite the importance of Sfps on female reproductive behavior in mosquitoes and other insects, the identity of Sfps in Ae. albopictus has not previously been reported. We used transcriptomics and proteomics to identify 198 Sfps in Ae. albopictus. We discuss possible functions of these Sfps in relation to Ae. albopictus reproduction-related biology. We additionally compare the sequences of these Sfps with proteins (including reported Sfps) in several other species, including Ae. aegypti. While only 72 (36.4%) of Ae. albopictus Sfps have putative orthologs in Ae. aegypti, suggesting low conservation of the complement of Sfps in these species, we find no evidence for an elevated rate of evolution or positive selection in the Sfps that are shared between the two Aedes species, suggesting high sequence conservation of those shared Sfps. Our results provide a foundation for future studies to investigate the roles of individual Sfps on feeding and reproduction in this mosquito. Functional analysis of these Sfps could inform strategies for managing the rate of pathogen transmission by Ae. albopictus.

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Section: Resultssupporting

confidence: 66%

Identification and Characterization of Seminal Fluid Proteins in the Asian Tiger Mosquito, Aedes albopictus

et al. 2014

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“…Knockdown of the AKHR in the cricket, G. bimaculatus, resulted in decreased levels of energy carrying molecules in the hemolymph and increased levels of triacylglycerol in the fat body. As expected, this also led to an increase of the feeding frequency of that insect (Konuma et al, 2012). Furthermore, post liver feeding decrease of the AKHR gene expression level corresponds well with the decrease of the AKH peptide observed in liver fed flesh flies (Bil et al, 2014).…”

Section: Discussionsupporting

confidence: 82%

Characterization of the adipokinetic hormone receptor of the anautogenous flesh fly, Sarcophaga crassipalpis

Bil

Timmermans

Verlinden

et al. 2016

Journal of Insect Physiology

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a b s t r a c tAdipokinetic hormone (AKH) is an insect neuropeptide mainly involved in fat body energy mobilization. In flies (Phormia regina, Sarcophaga crassipalpis), bugs (Pyrrhocoris apterus) and cockroaches (Periplaneta americana) AKH was also demonstrated to be involved in the regulation of digestion. This makes AKH an important peptide for anautogenous female flies that need to feed on a supplementary protein meal to initiate vitellogenesis, the large scale synthesis of yolk proteins and their uptake by the developing oocytes.Flesh fly AKH, originally identified as Phormia terraenovae hypertrehalosemic hormone (PhoteHrTH), functions through activation of the AKH receptor (AKHR). This is a G protein-coupled receptor that is the orthologue of the human gonadotropin-releasing hormone receptor. Pharmacological characterization indicated that the receptor can be activated by two related dipteran AKH ligands with an EC 50 value in the low nanomolar range, whereas micromolar concentrations of the Tribolium castaneum AKH were needed.Consistent with the energy mobilizing function of AKH, the receptor transcript levels were most abundant in the fat body tissue. Nonetheless, Sarcophaga crassipalpis AKHR transcript levels were also high in the brain, the foregut and the hindgut. Interestingly, the receptor transcript numbers were reduced in almost all measured tissues after protein feeding. These changes may enforce the use of ingested energy carrying molecules prior to stored energy mobilization.

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“…transcriptional silencing), are becomingly increasingly available in non-model organisms, including Nasonia , crickets, beetles and bugs (e.g. [71]–[77] ).…”

Section: Discussionmentioning

confidence: 99%

The Transcriptomic Basis of Oviposition Behaviour in the Parasitoid Wasp Nasonia vitripennis

et al. 2013

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Linking behavioural phenotypes to their underlying genotypes is crucial for uncovering the mechanisms that underpin behaviour and for understanding the origins and maintenance of genetic variation in behaviour. Recently, interest has begun to focus on the transcriptome as a route for identifying genes and gene pathways associated with behaviour. For many behavioural traits studied at the phenotypic level, we have little or no idea of where to start searching for “candidate” genes: the transcriptome provides such a starting point. Here we consider transcriptomic changes associated with oviposition in the parasitoid wasp Nasonia vitripennis. Oviposition is a key behaviour for parasitoids, as females are faced with a variety of decisions that will impact offspring fitness. These include choosing between hosts of differing quality, as well as making decisions regarding clutch size and offspring sex ratio. We compared the whole-body transcriptomes of resting or ovipositing female Nasonia using a “DeepSAGE” gene expression approach on the Illumina sequencing platform. We identified 332 tags that were significantly differentially expressed between the two treatments, with 77% of the changes associated with greater expression in resting females. Oviposition therefore appears to focus gene expression away from a number of physiological processes, with gene ontologies suggesting that aspects of metabolism may be down-regulated during egg-laying. Nine of the most abundant differentially expressed tags showed greater expression in ovipositing females though, including the genes purity-of-essence (associated with behavioural phenotypes in Drosophila) and glucose dehydrogenase (GLD). The GLD protein has been implicated in sperm storage and release in Drosophila and so provides a possible candidate for the control of sex allocation by female Nasonia during oviposition. Oviposition in Nasonia therefore clearly modifies the transcriptome, providing a starting point for the genetic dissection of oviposition.

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Knockdown of the Adipokinetic Hormone Receptor Increases Feeding Frequency in the Two-Spotted Cricket Gryllus bimaculatus

Cited by 57 publications

References 44 publications

Identification and Characterization of Seminal Fluid Proteins in the Asian Tiger Mosquito, Aedes albopictus

Identification and Characterization of Seminal Fluid Proteins in the Asian Tiger Mosquito, Aedes albopictus

Characterization of the adipokinetic hormone receptor of the anautogenous flesh fly, Sarcophaga crassipalpis

The Transcriptomic Basis of Oviposition Behaviour in the Parasitoid Wasp Nasonia vitripennis

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