Knockdown of the small heat-shock protein p26 by RNA interference modifies the diapause proteome ofArtemia franciscana

Abstract

Embryos of the crustacean Artemia franciscana may arrest as gastrulae, forming cysts that enter diapause, which is a state of reduced metabolism and enhanced stress tolerance. Diapausing cysts survive physiological stresses for years due, in part, to molecular chaperones. p26, a small heat-shock protein, is an abundant diapause-specific molecular chaperone in cysts, and it affects embryo development and stress tolerance. p26 is therefore thought to influence many proteins in cysts, and this study was undertaken to determine how the loss of p26 by RNA interference (RNAi) affects the diapause proteome of A. franciscana. The proteome was analyzed by shot-gun proteomics coupled to differential isotopic labeling and tandem mass spectrometry. Proteins in the diapause proteome included metabolic enzymes, antioxidants, binding proteins, structural proteins, transporters, translation factors, receptors, and signal transducers. Proteins within the diapause proteome either disappeared or were reduced in amount when p26 was knocked down, or conversely, proteins appeared or increased in amount. Those proteins that disappeared may be p26 substrates, whereas the synthesis of those proteins that appeared or increased may be regulated by p26. This study provides the first global characterization of the diapause proteome of A. franciscana and demonstrates that the sHsp p26 influences proteome composition.

Bioresilience to Mercury Chloride of the Brine Shrimp Artemia Salina after Treatment with Homeopathic Mercurius Corrosivus

“…This sugar forms H bonds with proteins and phospholipids, forming a kind of bioglass, in a process called vitrification, allowing the maintenance of internal stability. 54,60,[67][68][69][70][71][72][73] Besides this first adaptative level of hatching reduction, another deeper level of hatching inhibition was revealed after the analysis of the whole population, independent of time and moon phase, with high statistical significance and large effect size against the controls, as observed after treatment with MC 30cH (►Fig. 2).…”

HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway

“…HSPB7, located on 1p36.13, is a member of heat shock protein (HSP) family that plays essential roles in protein folding and cellular protein homeostasis [26]. Heat shock proteins have been implicated in diverse range of cellular processes, such as stress response, cell proliferation, and apoptosis [27][28][29]. Recent studies have revealed that heat shock proteins also play crucial roles in osteogenic differentiation.…”

Environmental Homeopathy: Homeopathic Potencies Regulate the Toxicity and Growth of Raphidiopsis raciborskii (cyanobacteria) and can be Tracked Physico-Chemically. Part 1: Biological Results

“…Therefore, it was necessary to investigate the mechanisms involved through gene expression of HSPs, which actively participate in bioresilience processes in the genus Artemia. [11][12][13][14][15][60][61][62][63] Compared to the other treatments, an increase in Hsp26 and p26 gene expression was seen in cysts and nauplii treated with Isotherapic (R. raciborskii extract) 200cH. The protein p26 is a small HSP that is abundant in A. franciscana embryos during diapause.…”