2019
DOI: 10.21597/jist.446068
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Knockdown of TIGAR Induces Apoptosis and Autophagy with Modulates NF-κB and HO-1 Expression in A549 Lung Cancer Cells

Abstract: ÖZET: TP53 kaynaklı glikoliz ve apoptozis düzenleyici (TIGAR) protein, glikoz metabolizması sırasında Fru-2, 6-P2 seviyelerini glukoz metabolizması sırasında kontrol eder ve nikotinamid adenin dinükleotit fosfat (NADPH) düzeyini devam ettirerek hücre içi anahtar bir antioksidan olan glutatyonun (GSH) geri dönüştürmesine yardımcı olur. Bu çalışma, A549 hücre hattında TIGAR'ın susturulmasının altında yatan, reaktif oksijen türleri (ROS) aracılı apoptotik ve otofajik mekanizmaları araştırmak için tasarlanmıştır. … Show more

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“…The A549 cells were cultured in DMEM medium (hyclone) containing 10% fetal bovine serum (FBS, hyclone in Logan, UT, USA) in a humidified 5% CO 2 incubator at 37 • C [13]. Then, the A549 cells were seeded in 6-well plates and the transfection of siRNA was performed using Lipofectamine 2000 (Invitrogen, Waltham, MA, USA) when the cells covered more than 50% of the plates.…”
Section: Cell Culture and Transfectionmentioning
confidence: 99%
“…The A549 cells were cultured in DMEM medium (hyclone) containing 10% fetal bovine serum (FBS, hyclone in Logan, UT, USA) in a humidified 5% CO 2 incubator at 37 • C [13]. Then, the A549 cells were seeded in 6-well plates and the transfection of siRNA was performed using Lipofectamine 2000 (Invitrogen, Waltham, MA, USA) when the cells covered more than 50% of the plates.…”
Section: Cell Culture and Transfectionmentioning
confidence: 99%