Knockout of acetoacetate degradation pathway gene atoDA enhances the toxicity tolerance of Escherichia coli to isopropanol and acetone

One-Pot Biosynthesis of Acetone from Waste Poly(hydroxybutyrate)

“…This observation is in line with a previous study reporting the growth of E. coli DH5α and MG1655 to OD 600 > 2 in 300 mM acetone, which in combination with our data suggests that acetone toxicity is not a major bottleneck to the pathway . We also explored the alternative hypothesis that acetoacetate was being consumed as a carbon source for microbial growth, thereby accounting for loss of yield at higher PHB loadings .…”

“…This observation is in line with a previous study reporting the growth of E. coli DH5α and MG1655 to OD 600 > 2 in 300 mM acetone, which in combination with our data suggests that acetone toxicity is not a major bottleneck to the pathway. 57 We also explored the alternative hypothesis that acetoacetate was being consumed as a carbon source for microbial growth, thereby accounting for loss of yield at higher PHB loadings. 58 To test this, we measured the growth of strains expressing no pathway enzymes (_pRSF), the full pathway (_pAc), or the 3-HB dehydrogenase only (_pHBDH Pf ) in minimal media containing 3-HB as the sole carbon source, theorizing that growth would be indicative of diversion of 3-HB-derived carbon flux toward biomass accumulation.…”

Highly efficient biosynthesis of 2,4-dihydroxybutyric acid by a methanol assimilation pathway in engineered Escherichia coli

“…34 Ethanol dehydrogenase (encoded by adhE) plays a role in producing ethanol from pyruvate via acetyl-CoA, and is responsible for the accumulation of acetyl-CoA. 35,36 Since in this study pyruvate is a key precursor of 2,4-DHB biosynthesis, an E. coli chassis for the accumulation of pyruvate is essential for improving the production. Hence, the pyruvate catabolism related genes poxB, pflB and adhE were sequentially knocked out in E. coli W3110 to inhibit the metabolic branch flux of pyruvate, resulting in E. coli D2, D4 and D6 (Fig.…”

Synthetic Biology and Metabolic Engineering Employing Escherichia coli for C2–C6 Bioalcohol Production

“…Transcriptome analysis revealed that genes related to amino acid biosynthesis, iron homeostasis, and energy metabolisms are related to isopropanol tolerance. Zhou et al (2019) used a targeted deletion approach and demonstrated that isopropanol tolerance could be increased by inactivation of the acetoacetyl-CoA transferase genes and atoDA, enabling growth in 500 mM isopropanol.…”