Knockout of t1r1 gene in zebrafish (Danio rerio) by CRISPR/Cas9 reveals its roles in regulating feeding behavior

Cai, Wenjing; Li, Jiao; Li, Ling; Xu, Chen; Wei, Jun-Ran; Yin, Zhan; He, Shan; Liang, Xu‐Fang

doi:10.1016/j.aquaculture.2021.737189

Cited by 8 publications

(2 citation statements)

References 38 publications

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“…This discrepancy may be because of the use of chimeric G‐protein (G16gust44), which does not exist in actual fish taste receptor cells. In fact, although zfT1R1/zfT1R3 does not respond to any l ‐amino acids when co‐expressed with chimeric G‐protein [ 4 ], t1r1 ‐KO zebrafish showed significantly reduced preference for L‐Ala, suggesting a role for zfT1R1 in feeding behavior in the natural environment [ 42 ]. In zebrafish, the absence of an ortholog of mammalian gustducin and expression of other G‐proteins have also been reported [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Zebrafish and medaka T1R (taste receptor type 1) proteins mediate highly sensitive recognition of l‐proline

2023

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In vertebrates, nutritional tastants, such as amino acids and sugars, are recognized by G‐protein‐coupled receptors of the taste receptor type 1 (T1R) family. Previous studies have shown that fish T1Rs are functionally distinct from mammalian T1Rs in certain regards. Here, we report the existence of oral receptors with high sensitivity to amino acids in zebrafish and medaka fish. We describe the construction of multiple cell lines stably expressing functional T1Rs (from medaka fish or zebrafish) with a chimeric G‐protein (G16gust44) using the Flp‐In system. Through functional assays with these cell lines, medaka fish and zebrafish were confirmed to possess particular T1Rs highly sensitive to l‐proline, possibly reflecting the physiological importance of l‐proline in teleosts, in line with previous studies.

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Section: Discussionmentioning

confidence: 99%

Zebrafish and medaka T1R (taste receptor type 1) proteins mediate highly sensitive recognition of l‐proline

2023

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“…In zebrafish, the role of the t1r1 gene of taste receptor type 1 in fish food behavior has been investigated [ 280 ]. Mutants with a knockout variant of this gene lose sensitivity to alanine, causing the fish to readily switch to a plant-based diet.…”

Section: Genome Editing In Salmonidae and Cyprinidae Aquaculture Fish...mentioning

confidence: 99%

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Orlova,

Ruzina,

Emelianova

et al. 2024

Genes

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Aquaculture supplies the world food market with a significant amount of valuable protein. Highly productive aquaculture fishes can be derived by utilizing genome-editing methods, and the main problem is to choose a target gene to obtain the desirable phenotype. This paper presents a review of the studies of genome editing for genes controlling body development, growth, pigmentation and sex determination in five key aquaculture Salmonidae and Cyprinidae species, such as rainbow trout (Onchorhynchus mykiss), Atlantic salmon (Salmo salar), common carp (Cyprinus carpio), goldfish (Carassius auratus), Gibel carp (Carassius gibelio) and the model fish zebrafish (Danio rerio). Among the genes studied, the most applicable for aquaculture are mstnba, pomc, and acvr2, the knockout of which leads to enhanced muscle growth; runx2b, mutants of which do not form bones in myoseptae; lepr, whose lack of function makes fish fast-growing; fads2, Δ6abc/5Mt, and Δ6bcMt, affecting the composition of fatty acids in fish meat; dnd mettl3, and wnt4a, mutants of which are sterile; and disease-susceptibility genes prmt7, gab3, gcJAM-A, and cxcr3.2. Schemes for obtaining common carp populations consisting of only large females are promising for use in aquaculture. The immobilized and uncolored zebrafish line is of interest for laboratory use.

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Application of CRISPR-Cas9 for Functional Analysis in A. mexicanus

Sifuentes-Romero¹,

Ferrufino²,

Kowalko³

2023

Neuromethods

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Knockout of t1r1 gene in zebrafish (Danio rerio) by CRISPR/Cas9 reveals its roles in regulating feeding behavior

Cited by 8 publications

References 38 publications

Zebrafish and medaka T1R (taste receptor type 1) proteins mediate highly sensitive recognition of l‐proline

Zebrafish and medaka T1R (taste receptor type 1) proteins mediate highly sensitive recognition of l‐proline

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Application of CRISPR-Cas9 for Functional Analysis in A. mexicanus

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