Knockouts of positive and negative elements of the circadian clock disrupt photoperiodic diapause induction in the silkworm, Bombyx mori

Photoperiodism of Diapause Induction in the Silkworm, Bombyx mori

“…Similar results were obtained by KO of other core circadian genes such as tim, Clock, and cycle (Tobita and Kiuchi, 2022). This evidence suggests that the circadian clock is not just related to the PR induction through the pleiotropic function of one or a few genes, but is fully integrated into the mechanism (Denlinger, 2017).…”

“…Recently, three groups reported the effects of KO of the per gene on silkworm PR using different races (Cui et al, 2021;Ikeda et al, 2021;Tobita and Kiuchi, 2022). Ikeda et al (2021) generated a per KO strain using TALEN-mediated genome editing technology with the Kosetsu race.…”

“…5B). Another research group, Tobita and Kiuchi (2022), generated KO mutants of per gene using the CRISPR/Cas9 method with the Daizo race. In the KO mutant, the diapauseproducing action of SD during the larval stage and that under LL conditions during the embryonic stage were completely lost (Fig.…”

Light pollution disrupts circadian clock gene expression in two mosquito vectors during their overwintering dormancy

“…pipiens f. molestus that found cyc to be upregulated and per to be downregulated in the presence of ALAN 43 . Additionally, knockout or knockdown of per and tim also decreased diapause incidence in the silkworm Bombyx mori 61 , and Cx. pipiens 36 , indicating that lower levels of these genes are associated with averting diapause.…”

“…albopictus . The circadian clock has been now linked to the diapause response in insects that enter embryonic, larval or nymphal, and adult diapauses 29 , 61 . Further, studies have found that ALAN inhibits diapause in insect that diapause as eggs, pupae, and adults 7 , 9 , 10 .…”