Objectives: To assess parents’ perceptions about amblyopia and its causes, symptoms, risk factors, treatments, and the importance of follow-up and to evaluate their awareness regarding the consequences and critical complications.Results: Parents or their companions (n=401) were surveyed, and 52.9% were mothers, 81.8% of mothers and 85.7% of fathers were highly educated (diploma, college or university degree), and 58 (14.5%) had a child who was afflicted with amblyopia. The knowledge subscale (nine items) showed acceptable reliability. Twenty percent of the participants declared having knowledge about amblyopia, but assessment by item showed low percentages of an adequate knowledge level, ranging from 0% for amblyopia causes, 35.9% for definition, and 59.9% for whether amblyopia is a hereditary disease. Thus, the rate of adequate knowledge (KS≥8) was 25.9%, and was associated with parents’ nationality (p<0.05) and self-declared knowledge about amblyopia (p<0.001). No significant difference was observed among respondents who had a child with amblyopia. There was a lack of knowledge about basic information and different aspects of the disease, which emphasized the relevance of school-based screening programs and adequate use of trustworthy internet resources using easily understood medical information.