Background: Inspiration among and participation of medical students is essential to make the “voluntary blood donation” movement a success. By assessment of knowledge, attitude and practice of blood donation, donor mobilization and retention strategies can be designed and optimally modified. In this study, knowledge about voluntary blood donation in medical interns was undertaken because by the end of their MBBS course they are supposed to have understood the importance of blood donation and become capable motivators for blood donation amongst potential donors in public.Methods: A cross-sectional descriptive study was conducted on 111 interns of Topiwala National Medical College, Mumbai using universal sampling method. Self-administered structured questionnaire was used to collect data.Results: Mean age of interns was 23.13 years with standard deviation of 0.832 of which girls comprised 48.6%. Forty seven point seven percent interns had donated blood before. The correct knowledge was prevalent in 45.0% only. Practice of blood donation amongst interns had male propensity and positive correlation with gender difference (p value 0.001). Most interns (79.3%) donated for moral satisfaction and social responsibility The commonest reason in the non-donors, for not donating was being medically unfit for donating (69.0%).Conclusions: Almost half (55.0%) of the medical students under study didn’t have adequate knowledge on blood safety and donor eligibility. Majority of them intended to donate blood in future however, a very few had ever actually donated blood. Increased awareness about blood donation among medical interns is expected to have amplified effects in the society, as an aware and motivated doctor can motivate and mobilize many others for voluntary non-remunerated blood donation and thus blood safety.