Nanotechnology is often described as an emerging technology, holding promise for a diverse range of fields. Public awareness may have a strong impact on public acceptance of nano-technology and its various implications. In Jordan, nano-education has only been introduced recently into universities and it is offered to students of pharmacy, engineering, biomedical sciences, and agriculture. However, there is no data available on nanotechnology awareness among the public in Jordan. Therefore, we conducted this study to evaluate Jordanian public awareness and concern about nanotechnology, nanomaterials, and nanoproducts and its implications for health related applications of nanotechnology. An online survey was developed consisting of 15 questions and separated into three domains. The study findings show that more than half of the respondents have a very low awareness with regards to nanotechnology and its various implications. Additionally, respondents show interest to learn more about nanotechnology and its implications, preferring several sources of information such as media, universities and research institutions.