Introduction. In the case of illness or injury, athletes, like other patients, seek medical care from general practitioners. Athletes, however, need to be aware of anti-doping regulations. The aim of this study was to evaluate the knowledge and attitudes to doping in sports among general practitioners in Vojvodina. Material and Methods. This cross-sectional study included 276 participants of both sexes and different ages. The data collection was performed using a questionnaire, as a non-standard research technique. The statistical analysis correlated the gathered data in regard sex and age of examinees. Results. Only a small number of respondents is well informed about both the List of Prohibited Doping Substances and Methods and the Law on Prevention of Doping in Sports (10.5% and 8%, respectively). Also, only 2.5% of examinees thought they were qualified to treat athletes. Correct answers that furosemide, pseudoephedrine and tamoxifen are prohibited in sports were given by a small number of respondents (36.6%, 56.9% and 29%, respectively). On the other hand, the fact that inhaled salmeterol, inhaled corticosteroids, enalapril and diclofenac are allowed in sports, was known by 42%, 40.6%, 60.9% and 52.9% of respondents, respectively. Doctors had different attitudes towards doping in sports. Conclusion. The results of this study showed that general practitioners have insufficient knowledge on different aspects of doping in sports. Since they treat all patients, including athletes, their knowledge should be on a higher level in order to avoid accidental doping. The obtained results point to the need for further education of general practitioners on doping in sports.