Knowledge‐Based Conceptual Synthesis of Industrial‐Scale Downstream Processes for Biochemical Products

Backhaus, Karin; Lochmüller, Mirka; Arndt, Markus C.; Riechert, Ole; Schembecker, Gerhard

doi:10.1002/ceat.201400111

Cited by 4 publications

(6 citation statements)

References 32 publications

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“…The first step in designing a downstream process is to characterize the stream coming from upstream processing. The factors essential for this are as follows (Nfor et al, 2008): (Asenjo and Andrews, 2008;Backhaus et al, 2015;Lienqueo and Asenjo, 2000;Petrides, 2013;Steffens et al, 2000). Thus, information from upstream aspects discussed in section 2 can play a vital role in characterizing the potential streams and designing separation systems for novel chemicals.…”

Section: Design Considerationsmentioning

confidence: 99%

A roadmap for the synthesis of separation networks for the recovery of bio-based chemicals: Matching biological and process feasibility

Yenkie

Clark

et al. 2016

Biotechnology Advances

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Microbial conversion of renewable feedstocks to high-value chemicals is an attractive alternative to current petrochemical processes because it offers the potential to reduce net CO emissions and integrate with bioremediation objectives. Microbes have been genetically engineered to produce a growing number of high-value chemicals in sufficient titer, rate, and yield from renewable feedstocks. However, high-yield bioconversion is only one aspect of an economically viable process. Separation of biologically synthesized chemicals from process streams is a major challenge that can contribute to >70% of the total production costs. Thus, process feasibility is dependent upon the efficient selection of separation technologies. This selection is dependent on upstream processing or biological parameters, such as microbial species, product titer and yield, and localization. Our goal is to present a roadmap for selection of appropriate technologies and generation of separation schemes for efficient recovery of bio-based chemicals by utilizing information from upstream processing, separation science and commercial requirements. To achieve this, we use a separation system comprising of three stages: (I) cell and product isolation, (II) product concentration, and (III) product purification and refinement. In each stage, we review the technology alternatives available for different tasks in terms of separation principles, important operating conditions, performance parameters, advantages and disadvantages. We generate separation schemes based on product localization and its solubility in water, the two most distinguishing properties. Subsequently, we present ideas for simplification of these schemes based on additional properties, such as physical state, density, volatility, and intended use. This simplification selectively narrows down the technology options and can be used for systematic process synthesis and optimal recovery of bio-based chemicals.

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Section: Design Considerationsmentioning

confidence: 99%

A roadmap for the synthesis of separation networks for the recovery of bio-based chemicals: Matching biological and process feasibility

Yenkie

Clark

et al. 2016

Biotechnology Advances

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“…Design approaches available for bioprocess development are typically classified into knowledge-based approaches like rulesof-thumbs [3,4] and expert systems [5,6], model-based approaches like optimization [7,8] and superstructures [9], and experimental approaches like design of experiments [10] and high-throughput screening [11,12]. A comprehensive overview of these design approaches is given by Nfor et al [13].…”

Section: Introductionmentioning

confidence: 99%

Production Rate‐Dependent Key Performance Indicators for a Systematic Design of Biochemical Downstream Processes

Brandt

Schembecker

2016

Chem Eng & Technol

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Key performance indicators have become a common tool in bioprocess development, as they allow an objective and independent rating and ranking of process alternatives screened. A disadvantage of all key performance indicators developed so far is their strong focus on costs, which makes them neglect the influence of the production rate on the profit of the process. But, since both production costs and production rate have an equally strong impact, both should be considered when decisions have to be made. Therefore, two new key performance indicators are introduced in this work, one for batch and one for continuous processing. They combine product yield, purity performance, variable manufacturing costs, and production rate to one objective. Thereby, misguided trade-offs between these four individual measures can be replaced by one key performance indicator. This ensures a systematic development of biochemical downstream processes.

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“…A bioreactor effluent is often dilute (less than 20 wt% product) [17] and the purity requirement for chemicals is relatively high. Therefore, downstream separation tends to be expensive, accounting for 60-80% of the total production cost in many cases [10,18,19]. Thus, the synthesis of an effective downstream bio-separation process is a critical but at the same time challenging task because multiple technologies are usually available for a given separation task, and thus a large number of alternative process networks exists.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, most products are initially produced intracellularly, but some products are localized extracellularly to the aqueous medium through passive diffusion or active transport [52]. Previous work on economic assessment for the separation of extracellular chemicals has been mainly restricted to specific examples such as hyaluronic acid [53][54][55][56][57], limonene [58][59][60][61], xanthan gum [62,63], butanediol [64][65][66][67], lactic acid [68][69][70][71][72] and penicillin V [19,73,74]. Also, assessment studies have been performed for individual separation technologies [75][76][77].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Yenkie

Maravelias

2019

BMC Chem Eng

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Recent advances in metabolic engineering have enabled the production of chemicals via bio-conversion using microbes. However, downstream separation accounts for 60-80% of the total production cost in many cases. Previous work on microbial production of extracellular chemicals has been mainly restricted to microbiology, biochemistry, metabolomics, or techno-economic analysis for specific product examples such as succinic acid, xanthan gum, lycopene, etc. In these studies, microbial production and separation technologies were selected apriori without considering any competing alternatives. However, technology selection in downstream separation and purification processes can have a major impact on the overall costs, product recovery, and purity. To this end, we apply a superstructure optimization based framework that enables the identification of critical technologies and their associated parameters in the synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions. We divide extracellular chemicals into three categories based on their physical properties, such as water solubility, physical state, relative density, volatility, etc. We analyze three major extracellular product categories (insoluble light, insoluble heavy and soluble) in detail and provide suggestions for additional product categories through extension of our analysis framework. The proposed analysis and results provide significant insights for technology selection and enable streamlined decision making when faced with any microbial product that is released extracellularly. The parameter variability analysis for the product as well as the associated technologies and comparison with novel alternatives is a key feature which forms the basis for designing better bioseparation strategies that have potential for commercial scalability and can compete with traditional chemical production methods.

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Knowledge‐Based Conceptual Synthesis of Industrial‐Scale Downstream Processes for Biochemical Products

Cited by 4 publications

References 32 publications

A roadmap for the synthesis of separation networks for the recovery of bio-based chemicals: Matching biological and process feasibility

A roadmap for the synthesis of separation networks for the recovery of bio-based chemicals: Matching biological and process feasibility

Production Rate‐Dependent Key Performance Indicators for a Systematic Design of Biochemical Downstream Processes

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

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