Purpose:The loss of knowledge poses a significant threat to organizational performance. Thus, this study examines the intricate dynamics of knowledge retention and the mitigation of knowledge loss within the context of a Brazilian national public organization operating in multimodal transportation infrastructure. Design/Methodology/Approach: Using a qualitative approach, we reviewed documents, conducted interviews with managers, and employed observational techniques, subsequently subjecting the gathered data to content analysis and triangulation. Findings: Our investigation unveiled that critical knowledge is lost primarily due to the departure of individuals from the organization or its respective units, coupled with deficiencies in the various stages of the knowledge retention process. Furthermore, we