Knowledge Management (KM) is known to be key to the long-term management success of knowledge-based units. In educational settings, KM is the effective of information to improve the use and sharing of valuable knowledge and information flow. Effective knowledge management practices are defined by acquisition, retention, and sharing, amplifying staff and students' teaching and learning abilities. However ,KM barriers tend to persist, and the presence of barriers impedes the attainment of objectives in educational setting. In particular, while the academic research on KM barrier is available in other manufacturing indsury such as IT (Karamat et al., 2018), to the best of our knowledge the topic of KM barriers concentrated in education is a scarcity in published research studies (Veer Ramjeawon & Rowley, 2017), and the empirical study has never done before for the departmental level including in Myanmar. Therefore, this study aims to uncover the barriers of each micro-KM practice in an academic department. An exploratory case study was conducted by interviewing seven participants who are academic staff in the Department of Commerce, Yangon University of Economics. The participants revealed that individual factors underpin knowledge acquisition, organizational factors impact knowledge retention, and both individual and organization-level barriers manifest in the knowledge sharing process. Therefore, it is advocated that resources such as time, space, and autonomy for individual knowledge creation, as well as cultural intervention, are requisite for fostering an atmosphere of knowledge sharing at the departmental level. As research contribution, effective knowledge management practices can improve the current situation of the department because it can have a great impact on the departmental outcomes and performance, which in turn leads to the objectives of teriatory education.