Keeping in mind the fact that organizations in the region of Vojvodina are moving from a negotiated to market economy, this study aims to analyze how the dimensions of collaborative climate affect the various activities of knowledge management. The degree to which collaborative climate influences knowledge work is investigated on 190 participants in the region of the Province of Vojvodina (Serbia). Two standardized questionnaires were used to collect data: a knowledge management questionnaire, which included 32 questions, and a collaborative climate questionnaire of 20 questions. To test the hypothesis of the study, the technique used was descriptive statistics, confirmatory factor analysis, and structural equation modeling. The results show that only the organizational culture as a dimension of collaborative climate contributes to the explanation of all the activities of knowledge management, except the activity of leaving knowledge, which fails to achieve a statistical relationship with any dimension of collaborative climate. This study provides useful insights for practitioners wanting to improve an organization’s performance and to increase organizational sustainability and learning. It is a useful base to expand further research in a way of better understanding the impact that collaborative climate has on the implementation of knowledge management in a transition towards a knowledge economy.