Background: Chronic pancreatitis is complex conditions that are requiring multi-faceted care with a range of interventions from a variety of specialists. Aim: This study was aimed to evaluate the effect of implementing educational guidelines on nurse's performance for patients with chronic pancreatitis. Subjects and Method: A quasi-experimental research design was used with a purposive sample of nurses (50) of both sexes, who directly caring for pancreatic patient in medical department at Tanta University. Tools: Tool (I) Nurses knowledge assessment questionnaire sheet, it was be divided into two part: part (A) sociodemographic characteristics of nurses , part (B) nurses' knowledge questionnaire Tool (II) nurses practice observational checklist, and Tool (III) the comprehensive pain assessment tool (full COMPAT questionnaire). Results: The results revealed highly significant differences were observed in the mean score of nurses' knowledge, and practice in relation to nursing management throughout periods of the study since P=<0.001. according to the nurse total practice score throughout period of study, this result reveals that more than half (58%) had unsatisfactory score of practice about chronic pancreatitis care in the preprogram implementation compared with the majority (88%) of nurses had satisfactory practice score immediately and respectively (84%) after one month of program. Also, it was observed that the mean score increased immediately after program compared to pre-program and decreased gradually after one month of the study. Conclusion and Recommendations: it can be concluded that majority studied nurses had a high level of practice and good knowledge immediately and one month after program than pre implementation of educational program.