Background: Due to lack of knowledge and practice of mothers in the care of their children undergoing hemodialysis, this study aimed to evaluate the effect of health educational program on improving mother's knowledge and health care practices of children undergoing hemodialysis. Methods: One-group pretest/posttest quasi-experimental research design was utilized to accomplish the aim of this study. The study was carried out at the dialysis units affiliated to Al-Ahrar Hospital, and Zagazig University Hospital, which was conducted on on 50 mothers. Tool: A structured interviewing questionnaire sheet used for data collection. Results: Out of 50 participants, 26% of studied mothers had satisfactory knowledge about hemodialysis before the educational program implementation, compared with 86% immediately after the implementation. Furthermore, 30% of studied mothers had satisfactory reported practice about hemodialysis care before the educational program implementation, compared with 82% immediately after the implementation. Conclusion: Prominently, there was a highly statistically significant positive linear correlation between mother's knowledge and reported practices regarding hemodialysis care before and after the implementation of the educational programme at p<0.0. Therefore, the educational program effectively improves mother's knowledge and home care related practices.