Malaysia aims to end the Tuberculosis (TB) epidemic by the year 2035. However, to ensure a successful program for TB control, it is still a challenge to instil good self-preventive healthcare among patients. Therefore, the study seeks to address the knowledge gap among TB patients and their level of prevention practices which have not been studied extensively in Malaysia. Using questionnaires, this cross-sectional study was conducted among 1606 registered TB patients in selected states of Malaysia from May 2014 to March 2015. General knowledge of TB among TB patients was high - patients from Penang, who resided in suburban areas and self-employed revealed to have high TB knowledge than the others. Thus, by identifying how TB patients view their transmission risk and prevention, practices may contribute to an improved future screening option and enhanced education efforts for medical practitioners to develop more customized programs for TB patients.