Background: Molar Incisor Hypomineralization (MIH) is a growing problem, and its treatment is a challenge. The purpose was to evaluate and compare the perceptions, knowledge and clinical experiences of MIH between general dental practitioners (GDPs) and pediatric dentists (PDs) in Spain. Methods: All dentists belonging to the College of Dentists of the Region of Murcia, in the South-East of Spain, were invited to participate in a cross-sectional survey. They were asked to complete a 2-section questionnaire including socio-demographic profiles and knowledge, experience, and perceptions of MIH. Data were analyzed using the Pearson chi-square test, Fisher´s exact test and Cramer´s V test.Results: The overall response rate was 18.6% (214/1147). Most respondents were aged 31-40 years (44.86%), with more than 15 years of professional experience (39.72%). They worked mainly in the private sector (84.58%) and were licensed in Dentistry (74.30%): 95.45% of pediatric dentists had detected an increase in the incidence of MIH in recent years (p<0.001). Only 23.80% of GDPs claimed to have had some training course in MIH. With respect to the etiology, chronic medical conditions (p<0.05) and environmental pollutants (p<0.001) were the only factors that showed significant between-group differences. Durability (p<0.01) and remineralization potential (p<0.05) were the factors where there was a between-group difference when choosing the material for restoration. In the case of post-eruptive fractures and opacities the preferred material for both groups was resin-modified glass ionomer (RMGIC). However, in incisor lesions, composite was the material of choice for both groups, with significant differences (p<0.05) in the use of glass ionomer. Most respondents expressed a need for continuing education on MIH. Conclusion: Spanish dentists perceived an increase in the incidence of MIH. The material of choice is RMGIC for non-aesthetic sectors and composite for incisors. Both GDPs and PDs believe they need more training on the etiology, diagnosis and treatment of MIH.