Background: Do-Not-Resuscitate (DNR) is signed to prevent patients from receiving invalid treatment at the end of life or near death. Nurses play an important role to discuss the DNR with patients or the patients’ family members. However, research focusing on the relationship between the knowledge, attitude and practice of a DNR using path modeling in nursing staff is limited. Therefore, this study aimed to elucidate the effect of path modeling on the knowledge, attitude, and practice toward DNR among the Taiwanese nursing staff.Methods: This was a cross-sectional, descriptive design using a simple random sampling. Data on demographics, knowledge, attitude, and practice as measured by the DNR inventory (KAP-DNR), Mindful Attention Awareness Scale, General Self-Efficacy Scale, and Dispositional Resilience Scale was collected. Participants were 194 nursing staff from a medical center in northern Taiwan in 2019. We performed descriptive statistics, regression analysis, and path modeling using SPSS 22.0 and set p <0.05 as the statistical significance threshold. Results: The results showed that participation in DNR signature and education related to palliative care were positive significant predictors of knowledge towards DNR. The DNR predictors toward attitude included DNR knowledge, mindfulness, self-efficacy, dispositional resilience, and religious belief of nurses. Generally, the key predictors of DNR practice were DNR attitude, dispositional resilience, and male nurses. In path modeling, we identified that self-efficacy, dispositional resilience, master’s degree, and religious belief directly influenced practices constituting DNR. Conclusion: Based on the findings of this study, it is proposed that nurses should improve their self-efficacy and dispositional resilience. Encouraging staff to undertake further education and have religious beliefs can improve the practice of DNR and provide better end of life care.