Kockiozyma gen. nov., for Zygozyma suomiensis: the phylogeny of the Lipomycetaceous yeasts

Abstract

In phylogenetic trees based on 18S rRNA gene, 26S rRNA gene, mitochondrial small subunit rRNA gene and the EF-1α gene sequences, and the concatenated sequences of the latter four regions derived from the neighbor-joining method, the four species of the genus Zygozyma constituted four clusters, respectively, with low bootstrap values. In phylogenetic trees based on the concatenated sequences of 18S rRNA genes, 26S rRNA genes, mitochondrial small subunit rRNA genes and the EF-1α genes derived from the neighborjoining method, the maximum parsimony method and the maximum likelihood method, Lipomyces species including Lipomyces starkeyi (the type species of the genus Lipomyces), Lipomyces tetrasporus and so on, Lipomyces lipofer (0 Waltomyces lipofer), Lipomyces japonicus (0 Smithiozyma japonica), Babjevia anomala (0 Lipomyces anomalus) and Dipodascopsis uninucleata constituted their own respective clusters. The four species of the genus Zygozyma, viz., Zygozyma oligophaga (the type species of the genus Zygozyma), Zygozyma suomiensis, Zygozyma arxii (0 Kawasakia arxii) and Zygozyma smithiae (0 Limtongia smithiae) were separated from one another and phylogenetically independent from other Lipomycetaceous yeast species. From the data obtained, all four species of the genus Zygozyma were distinguished from one another at the generic level. The name of Kockiozyma was newly suggested for Zygozyma suomiensis, and Kockiozyma suomiensis was introduced as a new combination.

A genome-informed higher rank classification of the biotechnologically important fungal subphylum Saccharomycotina

“…Notes : Although the genera Dipodascopsis , Babjevia, and Kockiozyma were not included in these analyses, based on previous analyses ( Kurtzman et al 2007 , Yamazaki et al 2020 , Jindamorakot et al 2012 , Kurtzman et al 2011a ), it is clear that these genera belong to the family Lipomycetaceae and are therefore also included in the Lipomycetales .…”

<i>Nguyenibacter vanlangensis</i> gen. nov., sp. nov., an unusual acetic acid bacterium in the <i>α</i>-<i>Proteobacteria</i>

“…In the experiment, the three dendrograms obtained showed similar patterns in the three regions. Among the yeasts examined, similar patterns were not necessarily seen in the three dendrograms (Jindamorakot et al, 2012;Yamada and Nagahama, 1991;Yamada and Nogawa, 1995). Recently, the socalled D1/D2 region sequences of the large subunit of rRNA or 26S rRNA genes have been widely used in yeast systematics (Fell, 1993;Kurtzman and Robnett, 1998;Kurtzman et al, 2011).…”

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“…In the family Lipomycetaceae Novak Zsolt, the seven genera Waltomyces, Zygozyma, Babjevia, Smithiozyma, Kawasakia, Limtongia and Kockiozyma were once introduced [2][3][4][5][6][7][8]. All of them were respectively taxonomic-homogeneous natured, i.e., morphologically, physiologically, chemotaxonomically, genetically and phylogenetically.…”

“…However, Kurtzman et al divided the family into two, i.e., the monophyletic genus Lipomyces sensu and the paraphyletic genus Dipodascopsis emend instead [9,10]. This paper describes the historical surveys in the systematics of the Lipomycetaceous yeasts and recovers the seven genera mentioned above as taxonomic criteria, in addition to the genus Lipomyces sensu stricto, the monotypic genus Dipodascopsis and the new genus Neoaidaea from the phylogenetic and the phenotypic points of view [1,7,8]. Accordingly, the family is comprised of ten genera in total as follows.…”

“…Lipomyces okinawensis Yamazaki, Yanagiba et Naganuma [14] The species classified in the genus Lipomyces sensu stricto were tightly coupled with one another in all the three phylogenetic trees based on the concatenated four-gene sequences of 18S rRNA, 26S rRNA, mitochondrial small subunit rRNA, and EF1-a derived from the neighbour-joining, the maximum parsimony and the maximum likelihood methods [8]. On the other hand, the phylogenetic positions of the Lipomyces species newly designated by Kurtzman et al were quite changeable in the monophyletic genus Lipomyces sensu [8,9]. In fact, the resulting genus has an extremely wide range of DNA G+C contents (14.2 mol%, from 41.5 -55.7 mol% G+C; Jindamorakot et al 2012) and three kinds of ubiquinone isoprenologues [15,16].…”