Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample

Reddy, G. S. N.; Prakash, Jogadhenu S. S.; Prabahar, Vadivel; Matsumoto, Genki I.; Stackebrandt, Erko; Shivaji, Sisinthy

doi:10.1099/ijs.0.02336-0

Cited by 104 publications

(69 citation statements)

References 23 publications

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“…Based on these data and on the occurrence of characteristic peptides in the partial hydrolysate of the peptidoglycan (data not shown), the peptidoglycan type is consistent with the type Lys-Ala 3 , variation A3a (Schleifer & Kandler, 1972), A11.6 according to the DSMZ Catalogue of strains (DSMZ, 2001). The interpeptide bridge of the peptidoglycan of strain CCM 132 containing three alanine residues (type A11.6) was also detected in the type strains of Kocuria polaris (Reddy et al, 2003), Kocuria palustris and K. rhizophila (Kovács et al, 1999), and in Kocuria rosea DSM 11630 (Schleifer & Kandler, 1972). However, the type strains of K. rosea and K. varians, as well as the K. varians strains DSM 20319 and DSM 20320, were reported to contain four alanine residues in the interpeptide bridge (type A11.7; Schleifer & Kandler, 1970, 1972DSMZ, 2001).…”

mentioning

confidence: 96%

Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. nov.

Tvrzová

Schümann

Sedláček

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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confidence: 96%

Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. nov.

Tvrzová

Schümann

Sedláček

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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“…Yoon et al (2001) also identified a novel species, Sporosarcina aquimarina. In continuation of our work on the heterotrophic bacteria associated with cyanobacterial mat samples from the lakes of Antarctica (Reddy et al, 2000(Reddy et al, , 2002a(Reddy et al, , 2002b(Reddy et al, , 2003, seven psychrophilic bacteria from a pond in McMurdo Region, Antarctica, have been identified as strains of Sporosarcina.…”

mentioning

confidence: 69%

“…The genus Sporosarcina originally comprised two species, Sporosarcina ureae and Sporosarcina halophila (Claus et al, 1983;Claus & Fahmy, 1986). However, three species of the genus Bacillus, namely Bacillus globisporus (Larkin & Stokes, 1967) In continuation of our work on the heterotrophic bacteria associated with cyanobacterial mat samples from the lakes of Antarctica (Reddy et al, 2000(Reddy et al, , 2002a(Reddy et al, , 2002b(Reddy et al, , 2003, seven psychrophilic bacteria from a pond in McMurdo Region, Antarctica, have been identified as strains of Sporosarcina. …”

mentioning

confidence: 77%

Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the McMurdo Dry Valleys, Antarctica

Reddy

Matsumoto

Shivaji

2003

International Journal of Systematic and Evolutionary Microbiology

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Strain CMS 21w T was isolated from a cyanobacterial mat sample taken from a pond located in the McMurdo Dry Valleys, Antarctica. Based on its phenotypic, chemotaxonomic and phylogenetic properties, strain CMS 21w T was identified as a member of the genus Sporosarcina. At the 16S rRNA gene level, CMS 21w T exhibited about 93-96 % similarity to all reported species of Sporosarcina and exhibited a maximum similarity of 96 % to both Sporosarcina globispora and Sporosarcina psychrophila. Based on more than 3 % difference at the 16S rRNA gene sequence level and the presence of distinct differences with respect to phenotypic, biochemical and chemotaxonomic features, strain CMS 21w T (=MTCC 4670 T =DSM 15428 T =CIP 107784 T ) is proposed as the type strain of a novel species of Sporosarcina, Sporosarcina macmurdoensis sp. nov.The genus Sporosarcina, which belongs to the family Bacillaceae, was created by Kluyver & van Niel (1936) to accommodate bacteria that have spherical or oval-shaped cells, low DNA G+C content (40-42 mol%) and MK-7 as the major menaquinone. Sporosarcina species can be differentiated from other members of the Bacillaceae by their coccoid or rod-shaped cells, motility, sporulation and possession of MK-7 as the major menaquinone and A4a as the peptidoglycan variant. The genus Sporosarcina originally comprised two species, Sporosarcina ureae and Sporosarcina halophila (Claus et al., 1983;Claus & Fahmy, 1986). However, three species of the genus Bacillus, namely Bacillus globisporus (Larkin & Stokes, 1967) In continuation of our work on the heterotrophic bacteria associated with cyanobacterial mat samples from the lakes of Antarctica (Reddy et al., 2000(Reddy et al., , 2002a(Reddy et al., , 2002b(Reddy et al., , 2003, seven psychrophilic bacteria from a pond in McMurdo Region, Antarctica, have been identified as strains of Sporosarcina. Source of the organisms, media and growth conditionsSeven pure cultures (CMS 13, CMS 14, CMS 15, CMS 21w T , CMS 89, CMS 91 and CMS 93) were isolated from cyanobacterial mat samples collected from ponds L4, L8 and E4 of Wright Valley, McMurdo Region, Antarctica (Matsumoto, 1993) according to a previously described procedure (Reddy et al., 2000) using ABM agar (0?5 % peptone, 0?2 % yeast extract and 1?5 % agar, pH 7?2; Shivaji et al., 1989). Optimum growth conditions were also determined using ABM agar plates.All seven isolates were identical with respect to their colony and cell morphologies, growth requirements, salt tolerance and many biochemical characteristics, suggesting that they are probably strains of the same species. In fact, all seven had identical 16S rRNA gene sequences, implying that the seven isolates are clonal in origin. CMS 21w T was chosen as a representative strain for detailed studies to establish its identity. Morphology, motility and biochemical and chemotaxonomic characteristicsThe morphology and motility of CMS 21w T were determined by taking exponential-phase cells and observing them by phase-contrast microscopy (10006). Biochemical tests were p...

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“…The major fatty acid is anteiso-C 15 : 0 (Stackebrandt et al, 1995). The DNA G+C content ranges from 66 to 75 mol% (Reddy et al, 2003). The genus currently comprises 18 recognized species with Kocuria rosea as the type species.…”

mentioning

confidence: 99%

Kocuria indica sp. nov., isolated from a sediment sample

Dastager

Tang

Krishnamurthi

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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An actinobacterial strain was isolated from a sediment sample from Chorao Island, in Goa province, India, and subjected to a taxonomic investigation. The isolate, designated NIO-1021 T , was a Gram-stain positive, aerobic, non-motile and coccoid. Strain NIO-1021 T was identified as a member of the genus Kocuria by a polyphasic approach. Strain NIO-1021 T could be differentiated from other members of the genus Kocuria on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain NIO-1021 T to the most closely related species, Kocuria marina KCTC 9943 T , was 98.6 % with 19 nt differences). Furthermore, DNA-DNA hybridization analysis revealed that the novel strain had lower relatedness with the type strains of other members of the genus Kocuria. The strain formed a monophyletic clade with K. marina with 100 % bootstrap values. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified lipids. The predominant menaquinone was MK-7(H 2 ). The major fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . The DNA G+C content of strain NIO-1021 T was 60.5 mol%. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification as representing a member of the genus Kocuria. On the basis of phenotypic, chemotypic and molecular characteristics, strain NIO-1021 T is considered to represent a novel species of the genus Kocuria, for which the name Kocuria indica sp. nov. is proposed, with strain NIO-1021 T (5NCIM 5455 T 5DSM 25126 T 5CCTCC AA 209050 T ) as the type strain.The genus Kocuria was first proposed by Stackebrandt et al. (1995) on the basis of a detailed phylogenetic and chemotaxonomic analysis of the genus Micrococcus (Stackebrandt et al., 1995). This led further to the description of the genera Dermacoccus, Kocuria, Kytococcus and Nesterenkonia. The genus Kocuria embraced three species formerly classified as members of the genus Micrococcus, i.e. Kocuria rosea (Micrococcus roseus; Flügge 1886), Kocuria varians (Micrococcus varians; Migula, 1900) and Kocuria kristinae (Micrococcus kristinae; Kloos et al., 1974). Based upon 16S rRNA gene sequence similarities the genus Kocuria represents one of the deepest lineages in the family Stackebrandt et al., 1995). The third clade includes only Kocuria palustris (Kovács et al., 1999). The last clade appears to be the deepest lineage in the family and comprises Kocuria atrinae (Park et al., 2010b) During an investigation exploring actinobacterial diversity in the marine environment of Chorao Island of Goa province, India, a coccoid actinobacterial strain was isolated and was subjected to a polyphasic investigation to determine its taxonomic position.Strain NIO-1021 T was isolated from a marine sediment sample taken from Chorao Island. After primary isolation and purification on marine agar 2216 (Difco) at 28 u C for 2 weeks, the purified strain was subcultured on the same medium and stored as slants at 4 u C and as 20 % (v/v) glycerol suspension...

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Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample

Cited by 104 publications

References 23 publications

Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. nov.

Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. nov.

Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the McMurdo Dry Valleys, Antarctica

Kocuria indica sp. nov., isolated from a sediment sample

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